LOCUS       sequence-173326-        7159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon 
            CH, Novina CD
  TITLE     The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved 
            SRA1-like Region.
  JOURNAL   Cell Rep. 2016 May 31;15(9):2025-37. doi: 
            10.1016/j.celrep.2016.04.018. Epub 2016 May 19.
  PUBMED    27210747
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.018"; journalName: "Cell Rep"; date: 
            "2016-05-31- 31"; volume: "15"; issue: "9"; pages: "2025-37"
COMMENT     Sequence Label: pCDNA3.1(-) + SLNCR1 _1521-2092
FEATURES             Location/Qualifiers
     source          1..7159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2136..2153)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2142..2366
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2412..2840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2499..2518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2709..2730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2849..2869)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2854..3183
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3034..3169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3096..3115
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3250..4044
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3304..3323)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3914..3933
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4218..4339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4255..4274)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4309..4328
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4388..4404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4388..4404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4401..4423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4412..4428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4436..4442,4443..4460,4461..4466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4481..4502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4619..4636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4790..5378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4870..4889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5549..6340,6341..6409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6172..6191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6410..6514)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6589..6608)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6780..7159
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacgggccc
      301 tctagactcg agcggccgcc actgtgctgg atatctgcag aattccacca cactggacta
      361 gtggatccga gctcggtacc aagcttcccg ggatacttag gatccagggc gcgcaggcgg
      421 cgcgggtgcg cggtgcggcg ctggtatcca gaggacgcgg tcaccgcctc tggcatttgt
      481 cgttctgcgc ttctccgcaa ggaccctctg ttaggcaggc gcccaccgta agcctcccgg
      541 gccttgtgaa cctgcaaacc caagtctgag agacgatccg ccttcagcgc tttccagctt
      601 ggcagagagg ctttcccggc ggggatcttt ggttggcgct ggcgatgcgc ggggaagaaa
      661 ggcgaggagc ggcgtccagg ctgggtgatg tcccagcacg agtaggcggg atgcgctcgc
      721 ttggtcctcc gggcgcccgg tccctgcccg cgtcgcgcgc ccacccctgg ggacgagaag
      781 gcggccgcct gaggaccccc gcccgcgacc tccgcgagtc tggagcgcag aggacagggt
      841 ctggctgctc tttggccttg gatggaaagt ggggaattgg gtggggggct gcggacccct
      901 taacgtggat tacttggtgt gtatcagctg ggctcagaag acccacgacc tcttctccat
      961 ccgtggattg atttgttctg cttaacagct gggtcgccaa gctggaggta tttttccctc
     1021 tccaccctgg tcttctcctg taacgtgtgg ccgccttttc cagcacggcc tcctgccttc
     1081 ctggtgcact ttttggagaa cgtggtggaa tcagaggttt ctggctgact cggtgggtgc
     1141 tttgaaccag gaaaggacaa gaaagaggat gggaaggact gatccacatt cccaccagga
     1201 agtttagcag aacccccgcg tgccacctgg accccttgga aggacctggc tcaggctgga
     1261 ccacctcttg agaggcagga gctctggatt tgatcaagaa ttctttgctg agcatggtgc
     1321 ctcatgccta taatcccaac actttgggag gccagtgtgg gaggatctct tgagcccagg
     1381 agttcaagac tagcctgggc aacacagaga gaccccatct ctaaaataat aataataata
     1441 aaataaaaaa ttagcagggc atggtggcat gtgcctgtag tcccagctac ccaggaggct
     1501 gaggcaagag gatggctgga gcctgggatg ttgaggctgc aatgaactgt gattacccca
     1561 ctgcactcca gcctgggcaa aagagcgaga gaccctgtct caaataataa taataataat
     1621 aatcttattt tggagaataa agagacctct ggatttgagg tgccatttgg gtagaaagaa
     1681 aagacgttta caccgagaaa tagtctgtgt tgccctgaag gagcagaggg atgcatcgct
     1741 ggaggtgacc tacagttgaa gaagactcat tatgacagac cttgtccttc ttccttgtgg
     1801 aaagtgtttc ctctgctgct actgctcatg agactcttcc ccctccctgt cccagggaac
     1861 caaagggctt tctaccacac cctttcttgc cccccgcctc ccatgtctgc tgtgcctttg
     1921 tactctgtag ccaacataca catgatttaa aaccctttct aaatatctat catggttcat
     1981 ccttgtccaa atgcagagtc agagctattt gtacttcatt attatttcca aggcgaatag
     2041 ttggctttct ttttgcaaaa ataattaaag tttttgtatg ttgcagttgc tctagataag
     2101 tatcacgtga agcttaagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca
     2161 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     2221 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     2281 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2341 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     2401 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2461 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2521 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2581 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2641 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2701 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2761 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2821 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     2881 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     2941 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3001 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3121 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3181 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     3241 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     3301 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     3361 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     3421 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     3481 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     3541 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     3601 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     3661 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     3721 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     3781 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     3841 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     3901 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     3961 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     4021 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     4081 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     4141 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     4201 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4261 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4321 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     4381 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     4441 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     4501 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4561 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4621 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4681 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4741 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4801 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4861 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4921 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4981 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5041 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5101 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5161 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5221 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5281 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5341 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5401 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5461 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5521 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5581 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5641 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5701 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5761 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5821 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5881 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5941 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6001 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6061 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6121 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6181 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6241 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6301 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6361 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6421 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6481 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6541 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     6601 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     6661 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     6721 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     6781 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6841 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     6901 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     6961 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     7021 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     7081 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     7141 agtcatcgct attaccatg
//
