LOCUS       sequence-173359-        2143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Obst U, Lu TK, Sieber V
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion 
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337.
  PUBMED    28252957
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337."
COMMENT     Sequence Label: pPTK002-2-pGAP
FEATURES             Location/Qualifiers
     source          1..2143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..477
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     terminator      complement(495..529)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(600..1259)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1174..1193
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1441..2029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1930..1949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttgtaga aatgtcttgg tgtcctcgtc caatcaggta gccatctctg aaatatctgg
       61 ctccgttgca actccgaacg acctgctggc aacgtaaaat tctccggggt aaaacttaaa
      121 tgtggagtaa tggaaccaga aacgtgtctt cccttctctc tccttccacc gcccgttacc
      181 gtccctagga aattttactc tgctggagag cttcttctac ggcccccttg cagcaatgct
      241 cttcccagca ttacgttgcg ggtaaaacgg aggtcgtgta cccgacctag cagcccaggg
      301 atggaaaagt cccggccgtc gctggcaata atagcgggcg gacgcatgtc atgagattat
      361 tggaaaccac cagaatcgaa tataaaaggc gaacaccttt cccaattttg gtttctcctg
      421 acccaaagac tttaaattta atttatttgt ccctatttca atcaattgaa caactattat
      481 gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      541 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      601 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
      661 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      721 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      781 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
      841 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      901 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
      961 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1021 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1081 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1141 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1201 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1261 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1321 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     1381 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1441 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1501 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1561 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1621 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1681 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1741 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1801 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1861 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1921 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1981 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2041 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2101 gttatcccct gattctgtgg ataaccgtag tcggtctcaa acg
//
