LOCUS       sequence-173468-         396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning backbone for sgRNA, also has a barcode that allows detection
            from scRNA-seq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Xie S, Duan J, Li B, Zhou P, Hon GC
  TITLE     Multiplexed Engineering and Analysis of Combinatorial Enhancer 
            Activity in Single Cells.
  JOURNAL   Mol Cell. 2017 Apr 20;66(2):285-299.e5. doi: 
            10.1016/j.molcel.2017.03.007. Epub 2017 Apr 13.
  PUBMED    28416141
REFERENCE   2  (bases 1 to 396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.03.007"; journalName: "Mol Cell"; date: 
            "2017-04-20- 20"; volume: "66"; issue: "2"; pages: "285-299.e5"
COMMENT     Sequence Label: Lenti-sgRNA(MS2)-puro-barcode
FEATURES             Location/Qualifiers
     source          1..396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     163..182
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        278..296
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        348..366
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga
       61 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
      121 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
      181 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      241 ggagacggga taccgtctct gttttagagc taggccaaca tgaggatcac ccatgtctgc
      301 agggcctagc aagttaaaat aaggctagtc cgttatcaac ttggccaaca tgaggatcac
      361 ccatgtctgc agggccaagt ggcaccgagt cggtgc
//
