LOCUS       sequence-17352-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, 
            Lawrence HJ, Cleary ML, Largman C
  TITLE     Hox homeodomain proteins exhibit selective complex stabilities with 
            Pbx and DNA.
  JOURNAL   Nucleic Acids Res 1996 Mar 1;24(5):898-906.
  PUBMED    8600458
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1996-03-1"; volume: "24(5)"; pages: "898-906"
COMMENT     Sequence Label: HOXB3 (human) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..22
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             26..58
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             311..328
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(381..399)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      395..442
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      479..838
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(566..585)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     776..797
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggcctggtg ccgcgcggca gccatatggc tagcatgact ggtggacagc aaatgggtcg
       61 ggatccgaat tctaccatgg agaaagccac ctactccgac aacgccgcgg ctgctctctt
      121 cggaggctat tcctcgtacc ctggcagcaa tggcttcggc ttcgatgtcc ccccccaacc
      181 cccatttcag gccgccacgc acctggaggg cgactaccag cgctcagctt gctcgctgca
      241 gtccctgggc aacgctgccc acatgccaag agcaaggagc tccgtcgaca agcttgcggc
      301 cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      361 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      421 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
      481 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      541 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      601 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      661 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      721 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      781 ctcttgttcc aaactggaac aacactcaac ccctatctcg gtctattctt ttgatttata
      841 agggatttt
//
