LOCUS       sequence-173536-        2729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express csgB curli protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdelwahab MT, Kalyoncu E, Onur T, Baykara MZ, Seker UO
  TITLE     Genetically-Tunable Mechanical Properties of Bacterial Functional 
            Amyloid Nanofibers.
  JOURNAL   Langmuir. 2017 Apr 7. doi: 10.1021/acs.langmuir.7b00112.
  PUBMED    28388843
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Langmuir. 
            2017 Apr 7. doi: 10.1021/acs.langmuir.7b00112."
COMMENT     Sequence Label: pZa-tetO-csgB-CmR
FEATURES             Location/Qualifiers
     source          1..2729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      11..97
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(133..150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(267..812)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(347..366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(926..1020)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1041..1700)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1615..1634
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1701..1803)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    1842..1860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1867..1885
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(2147..2165,2166..2171,2172..2188,2189..2194,
                     2195..2220)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    2147..2165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2172..2190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2255..2266
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cgcgtggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
       61 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctaggggat
      121 atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga
      181 aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga
      241 agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac
      301 gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg
      361 tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
      421 attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc
      481 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt
      541 atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc
      601 agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa
      661 ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt
      721 tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc
      781 aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat
      841 atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac
      901 gatataagtt gttactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
      961 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     1021 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     1081 tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc
     1141 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     1201 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     1261 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     1321 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     1381 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     1441 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc
     1501 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     1561 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     1621 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     1681 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     1741 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     1801 tcaacgtctc attttcgcca gatatcgacg tctttgggcc ctccctatca gtgatagaga
     1861 ttgacatccc tatcagtgat agagatactg agcacagtcg acacccaaat ccaggaggtg
     1921 attggtagtg gtggttaatg aaaattaact tactactacc atatatctct agaaacagaa
     1981 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
     2041 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
     2101 atcaaataaa acgaaaggct cagtcgaaag actgggcctt ctcgagtccc tatcagtgat
     2161 agagattgac atccctatca gtgatagaga tactgagcac atcagcagga cgcactgacc
     2221 gaattctacc attcacctct tggatttggg tattaaagag gagaaaggta ccatgaaaaa
     2281 caaattgtta tttatgatgt taacaatact gggtgcgcct gggattgcag ccgcagcagg
     2341 ttatgattta gctaattcag aatataactt cgcggtaaat gaattgagta agtcttcatt
     2401 taatcaggca gccataattg gtcaagctgg gactaataat agtgctcagt tacggcaggg
     2461 aggctcaaaa cttttggcgg ttgttgcgca agaaggtagt agcaaccggg caaagattga
     2521 ccagacagga gattataacc ttgcatatat tgatcaggcg ggcagtgcca acgatgccag
     2581 tatttcgcaa ggtgcttatg gtaatactgc gatgattatc cagaaaggtt ctggtaataa
     2641 agcaaatatt acacagtatg gtactcaaaa aacggcaatt gtagtgcaga gacagtcgca
     2701 aatggctatt cgcgtgacac aacgttaaa
//
