LOCUS       sequence-173545-       12024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in 
            Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Makhijani K, To TL, Ruiz-Gonzalez R, Lafaye C, Royant A, Shu X
  TITLE     Precision Optogenetic Tool for Selective Single- and Multiple-Cell 
            Ablation in a Live Animal Model System.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):110-119. doi: 
            10.1016/j.chembiol.2016.12.010. Epub 2017 Jan 5.
  PUBMED    28065655
REFERENCE   2  (bases 1 to 12024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.12.010"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "110-119"
COMMENT     Sequence Label: pIHEU_miniSOG2 T2A H3.3A-EGFP
FEATURES             Location/Qualifiers
     source          1..12024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    1044..1138
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        1157..1395
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1342..1363
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             1406..1417
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     intron          1468..1600
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1583..1602
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1972..2025
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2497..2499,2500..2502,2503..3216)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2542..2563)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2803..2822)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3150..3171
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3878..4263
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(4273..4291)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4743..4814,5216..5489,5564..6218,6280..6595,6799..6930,
                     7001..7615)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8394..8948,8949..8979)
                     /label=P element 5' end
     rep_origin      complement(9214..9802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9294..9313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9973..10764,10765..10833))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10596..10615
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10834..10938)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11006..11024
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11062..11084)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11184..11203
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    11412..11481
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgcctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
     1081 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     1141 gagactctag cgagcgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
     1201 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
     1261 ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac
     1321 cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc aagaagtaat tattgaatac
     1381 aagaagagaa ctctgaatag ggaattggga attgcagtga ctctcttaag gtagccttgc
     1441 agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga
     1501 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
     1561 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat
     1621 tacagctcga attggggcgc gcccaaaatg gaaaaaagct tcgtgattac tgacccgcgc
     1681 ctgccggata atccgatcat cttcgcctcc gatagtttcc tggaactgac tgaatacagc
     1741 cgtgaggaaa ttctgggtcg taatccccgt ttcctgcggg gcccggaaac cgatcaagca
     1801 actgttcaga aaattcatga tgcaatccgt gatcaacgtg aaatcaccgt gcagctgatt
     1861 aactatacca aaagcggcaa gaaattctgg aacttgtttc gcctgcagcc gattcgcgat
     1921 cagaaaggtg agctgcagta cttcatcggg gttcagctgg atggtggatc cgagggcaga
     1981 ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccagaatt catggcacgt
     2041 accaagcaaa cagcccgtaa atcgaccgga ggcaaggcgc cccgcaagca gctggccacc
     2101 aaggcggccc gtaaatcggc gccatccacc ggcggagtga agaagccaca tcgctaccgt
     2161 cctggaacgg tggccctgcg tgagattcgt cgctaccaga agtccacgga gctgctcatc
     2221 cgcaagctgc cgttccagcg tctggtgcgc gagatagccc aggacttcaa gaccgatctg
     2281 cgcttccagt cggcggccat tggagcccta caggaggcca gcgaggcgta cctggtcggt
     2341 ctgttcgagg acaccaatct gtgcgccatt cacgccaagc gcgtcaccat tatgcccaag
     2401 gacatccagc tggccagacg catccgtggc gagcgggcct cacggccggt cgcaacaatg
     2461 agtctcgagt ctggtggcgg gagtggtggc gggtccatgg tgagcaaggg cgaggagctg
     2521 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     2581 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     2641 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     2701 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     2761 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2821 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2881 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2941 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     3001 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3061 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     3121 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3181 gggatcactc tcggcatgga cgagctgtac aagtaattaa ttaactaagc cagtcggcaa
     3241 tcggacgcct tcgaaacatg caacactaat gtttaattca gatttcagca gagacaagct
     3301 aaaacaccga cgagttgtaa tcatttctgt gcgccagcat atatttctta tatacaacgt
     3361 aatacataat tatgtaattc tagcatctcc ccaacactca catacaaaca aacaaaaaat
     3421 acaaacacac aaaacgtatt tacccgcacg catccttggc gaggttgagt atgaaacaaa
     3481 aacaaaactt aatttagagc aaagtaatta cacgaataaa tttaataaaa aaaaactata
     3541 ataaaaacgc caatcatgtt atttccaaaa aaaaaaaaaa aaaaaaaaac tagacgagat
     3601 gtttgcgctt gtgtcgatga atgtaaagaa ttgcaactac atagatggta attaatttca
     3661 gctgtaagaa gtacaagttt ttcataattg tgagaaaagt gcagtagtat cgcttaaagt
     3721 tcagttttaa tcagggcatt tagcagctcg aacccaatgt atttgtaagc ccaataaacg
     3781 tgtaaatgcc tagagcaaaa tatcacaact gtagcttttc atccgaaaga taataatgaa
     3841 tagactgacc tgacgttttt caaaatgcgt aactagtcac gtaataagtg tgcgttgaat
     3901 ttattcgcaa aaacattgca tattttcggc aaagtaaaat tttgttgcat accttatcaa
     3961 aaaataagtg ctgcatactt tttagagaaa ccaaataatt ttttattgca tacccgtttt
     4021 taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacttt gacgaaacaa
     4081 attttcgttg catacccaat aaaagattat tatattgcat acccgttttt aataaaatac
     4141 attgcatacc ctcttttaat aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc
     4201 atacccaata aaagattatt atattgcata ccttttcttg ccataccatt tagccgatca
     4261 attctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat aaaaaaaaca
     4321 tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt aaatttactt
     4381 cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca aaatgacatt
     4441 ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt
     4501 cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc
     4561 gccttcagtt gcattttgtc agcggtttcg tgacgaagct ccaagcggtt tacgccatca
     4621 attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt tgttttttga
     4681 gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg
     4741 caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac ccatctgccg
     4801 agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt gatctatagg
     4861 aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca
     4921 aaccgcaatt gcacaaacaa atagtgacac gaaacagatt attctggtag ctgttctcgc
     4981 tatataagac aatttttgag atcatatcat gatcaagaca tctaaaggca ttcattttcg
     5041 actatattct tttttacaaa aaatataaca accagatatt ttaagctgat cctagatgca
     5101 caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcggggtac tttttgttcg
     5161 gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat tgcagggtga
     5221 cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca aaaactacgg
     5281 cacgctccgg ccacccagtc cgccggagga ctccggttca gggagcggcc aactagccga
     5341 gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc agccgggctc
     5401 cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc gacacatacc
     5461 ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt
     5521 gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg cgtggcctat
     5581 ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat
     5641 gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc
     5701 aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat
     5761 gacctcttta tcggctccct aacggccagg gaacacctga ttttccaagc catggtgcgg
     5821 atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag
     5881 ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg
     5941 tccggcggag aaaggaagcg tctggcattc gcctccgagg ctctaaccga tccgccgctt
     6001 ctgatctgcg atgagcccac ctccggactg gactccttta ccgcccacag cgtcgtccag
     6061 gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct
     6121 tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc
     6181 ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg tgtttattaa
     6241 gggtatctag tattacataa catctcaact cctatccagc gtgggtgccc agtgtcctac
     6301 caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg gacgggagat
     6361 cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gccattagca aagtagcccg
     6421 ggatatggag cagttgttgg ccaccaaaaa tctggagaag ccactggagc agccggagaa
     6481 tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt ggcgatcctg
     6541 gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga caacggtgag
     6601 tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa ttcgctagat
     6661 tttagataga attgcctgat tccacaccct tcttagtttt tttcaatgag atgtatagtt
     6721 tatagttttg cagaagataa ataaatttca tttaactcgc gaatattaat gagatgcgag
     6781 taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt gggccaacaa
     6841 ctcacgcaag tgggtgtgat gaatatcaac ggagccatct tcctcttcct gaccaacatg
     6901 acctttcaaa acgtctttgc cacgataaat gtaagtcatg tttagaatac atttgcattt
     6961 caataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct cagagctgcc
     7021 agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa
     7081 aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg cgattgccta
     7141 tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac
     7201 tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac
     7261 ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt
     7321 cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt
     7381 ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat
     7441 tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct
     7501 taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag
     7561 cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac
     7621 atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg tgtttactgt
     7681 ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac
     7741 ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg gatttgtctt
     7801 ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta tcgctacata
     7861 aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct cttcataatt
     7921 cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc gctctccgca
     7981 acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg cgcgattcgg
     8041 ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta ataaatattt
     8101 gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag ctatatttat
     8161 tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg agtgattagc
     8221 gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa
     8281 aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta taataataag
     8341 aatacattta atttagaaaa tgcttggatt tcactggaac tagaattaat tcggctgctg
     8401 ctctaaacga cgcatttcgt actccaaagt acgaattttt tccctcaagc tcttattttc
     8461 attaaacaat gaacaggacc taacgcacag tcacgttatt gtttacataa atgatttttt
     8521 ttactattca aacttactct gtttgtgtac tcccactggt atagccttct tttatctttt
     8581 ctggttcagg ctctatcact ttactaggta cggcatctgc gttgagtcgc ctccttttaa
     8641 atgtctgacc ttttgcaggt gcagccttcc actgcgaatc attaaagtgg gtatcacaaa
     8701 tttgggagtt ttcaccaagg ctgcacccaa ggctctgctc ccacaatttt ctcttaatag
     8761 cacacttcgg cacgtgaatt aattttactc cagtcacagc tttgcagcaa aatttgcaat
     8821 atttcatttt tttttattcc acgtaagggt taatgttttc aaaaaaaaat tcgtccgcac
     8881 acaacctttc ctctcaacaa gcaaacgtgc actgaattta agtgtatact tcggtaagct
     8941 tcggctatcg acgggaccac cttatgttat ttcatcatgg gccagaccca cgtagtccag
     9001 cggcagatcg gcggcggaga agttaagcgt ctccaggatg accttgcccg aactggggca
     9061 cgtggtgttc gacgatgtgc agctaatttc gcccggctcc acgtccgccc attggttaat
     9121 cagcagaccc tcgttggcgt aacggaacca tgagaggtac gacaaccatt tgaggtatac
     9181 tggcaccgag cccgagttca agaagaaggc gtttttccat aggctccgcc cccctgacga
     9241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10141 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10201 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10261 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10321 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10381 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10441 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10501 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10561 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10621 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10681 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10741 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10801 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10861 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10921 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10981 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    11041 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    11101 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    11161 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc
    11221 gacatgcccg ccgtgaccgt cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc
    11281 gacgccgtcg cacgtcccgt gctcaccgtg accaccgcgc ccagcggttt cgagggcgag
    11341 ggcttcccgg tgcgccgcgc gttcgccggg atcaactacc gccacctcga cccgttcatc
    11401 atgatggacc agatgggtga ggtggagtac gcgcccgggg agcccaaggg cacgccctgg
    11461 cacccgcacc gcggcttcga gaccgtgacc tacatcgtcg atatgcggtg tgaaataccg
    11521 caccgaatcg cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt
    11581 gttgcggaga gcgggtggga gacagcgaaa gagcaactac gaaacgtggt gtggtggagg
    11641 tgaattatga agagggcgcg cgatttgaaa agtatgtata taaaaaatat atcccggtgt
    11701 tttatgtagc gataaacgag tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt
    11761 agaagacaaa tccaaagtct acttgtgggg atgttcgaag gggaaatact tgtattctat
    11821 aggtcatatc ttgtttttat tggcacaaat ataattacat tagctttttg agggggcaat
    11881 aaacagtaaa cacgatggta ataatggtaa aaaaaaaaaa caagcagtta tttcggatat
    11941 atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga
    12001 agctcacgat gagaatggcc agac
//
