LOCUS       sequence-173613-        8779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one vector for CRISPR/Cas9-mediated homology-independent 
            knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA 
            expression cassettes. The first gRNA cloning site is empty..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8779)
  AUTHORS   Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, 
            Nakayama K
  TITLE     Practical method for targeted disruption of cilia-related genes by 
            using CRISPR/Cas9-mediated homology-independent knock-in system.
  JOURNAL   Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 
            10.1091/mbc.E17-01-0051.
  PUBMED    28179459
REFERENCE   2  (bases 1 to 8779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 
            10.1091/mbc.E17-01-0051."
COMMENT     Sequence Label: peSpCas9(1.1)-2×sgRNA (empty, donor)
FEATURES             Location/Qualifiers
     source          1..8779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          6..233
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      245..254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             254..319
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             326..346
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(371..2908,2909..2911,2912..3373,3374..3376,3377..3544,
                     3545..3547,3548..4471)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4472..4519
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4547..4564)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4553..4760
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4769..4909
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4769..4898
                     /label=AAV2 ITR
     rep_origin      4984..5439
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5071..5090)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5281..5302
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5456..5475)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5575..5597
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5635..5653)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5721..5825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5826..5894,5895..6686)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6044..6063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6857..7445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7346..7365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7507..7755
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        7774..7849
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7940..8188
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        8209..8284
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8375..8660
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
       61 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
      121 ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg ttggttggtg
      181 gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttggac
      241 cggtgccacc atggactata aggaccacga cggagactac aaggatcatg atattgatta
      301 caaagacgat gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt
      361 cccagcagcc gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg
      421 ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac
      481 cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac
      541 agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg
      601 gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt
      661 ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat
      721 cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct
      781 gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct
      841 ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa
      901 cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga
      961 aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa
     1021 gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt
     1081 cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct
     1141 ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct
     1201 gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga
     1261 cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag
     1321 cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct
     1381 cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg
     1441 ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc
     1501 catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct
     1561 gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga
     1621 gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga
     1681 aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg
     1741 aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt
     1801 cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt
     1861 cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt
     1921 caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc
     1981 cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa
     2041 agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt
     2101 ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct
     2161 gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga
     2221 tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac
     2281 ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg
     2341 ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac
     2401 aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca
     2461 cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga
     2521 tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct
     2581 gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa
     2641 catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg
     2701 cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga
     2761 acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa
     2821 tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt
     2881 ggaccatatc gtgcctcaga gctttctggc cgacgactcc atcgacaaca aggtgctgac
     2941 cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa
     3001 gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga
     3061 caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa
     3121 gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg
     3181 gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct
     3241 gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat
     3301 caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat
     3361 caaaaagtac cctgcgctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt
     3421 gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt
     3481 ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg
     3541 gaaggcgcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg
     3601 ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac
     3661 cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa
     3721 gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac
     3781 cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa
     3841 gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc
     3901 catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct
     3961 gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg
     4021 cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct
     4081 ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt
     4141 tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa
     4201 gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg
     4261 ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct
     4321 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag
     4381 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac
     4441 acggatcgac ctgtctcagc tgggaggcga caaaaggccg gcggccacga aaaaggccgg
     4501 ccaggcaaaa aagaaaaagt aagaattcct agagctcgct gatcagcctc gactgtgcct
     4561 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4621 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4681 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagag
     4741 aatagcaggc atgctgggga gcggccgcag gaacccctag tgatggagtt ggccactccc
     4801 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     4861 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     4921 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4981 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5041 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5101 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5161 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     5221 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5281 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     5341 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     5401 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     5461 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5521 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5581 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5641 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5701 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5761 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5821 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5881 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5941 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6001 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6061 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6121 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6181 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6241 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6301 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6361 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6421 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6481 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6541 gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6601 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6661 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6841 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6901 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6961 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7021 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7081 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7141 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7201 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7261 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7321 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7381 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7441 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7501 acatgtgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     7561 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     7621 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     7681 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     7741 aaaggacgaa acaccgggtc ttcgagaaga cctgttttag agctagaaat agcaagttaa
     7801 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt
     7861 agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct
     7921 gcagacaaat ggctctagag agggcctatt tcccatgatt ccttcatatt tgcatatacg
     7981 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     8041 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     8101 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     8161 atatatcttg tggaaaggac gaaacaccgc atcgtacgcg tacgtgttgt tttagagcta
     8221 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     8281 gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc
     8341 gcgtgcgcca attctgcaga caaatggcgg tacccgttac ataacttacg gtaaatggcc
     8401 cgcctggctg accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga
     8461 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     8521 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     8581 ggcattgtgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     8641 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     8701 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     8761 tgggggcggg ggggggggg
//
