LOCUS       sequence-173653-        6357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6357)
  TITLE     PTEN
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1066 pBabe puroL PTEN
FEATURES             Location/Qualifiers
     source          1..6357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    64..263
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             464..880
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     810..832
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     848..864
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             912..938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             912..938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             918..944
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(2209..2229)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2214..2543
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2394..2529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2553..3152
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2553..2572)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3049..3069
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3373..3842
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3972..3989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4143..4731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4223..4242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4891..5682,5683..5751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5514..5533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5752..5856)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5887..6356
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga
       61 ggcaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt
      121 tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg
      181 aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg actttggggg
      241 ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata
      301 tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct
      361 ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg
      421 ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac
      481 tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc
      541 ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa
      601 cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa
      661 ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga
      721 agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc
      781 tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg
      841 atcctccctt tatccagccc tcactccttc tctaggcgcc ggccggatcg aagactaagc
      901 ttaccatggc ctacccctac gacgtgcccg actacgcctc cctcggatcc atgacagcca
      961 tcatcaaaga gatcgttagc agaaacaaaa ggagatatca agaggatgga ttcgacttag
     1021 acttgaccta tatttatcca aacattattg ctatgggatt tcctgcagaa agacttgaag
     1081 gcgtatacag gaacaatatt gatgatgtag taaggttttt ggattcaaag cataaaaacc
     1141 attacaagat atacaatctt tgtgctgaaa gacattatga caccgccaaa tttaattgca
     1201 gagttgcaca atatcctttt gaagaccata acccaccaca gctagaactt atcaaaccct
     1261 tttgtgaaga tcttgaccaa tggctaagtg aagatgacaa tcatgttgca gcaattcact
     1321 gtaaagctgg aaagggacga actggtgtaa tgatatgtgc atatttatta catcggggca
     1381 aatttttaaa ggcacaagag gccctagatt tctatgggga agtaaggacc agagacaaaa
     1441 agggagtaac tattcccagt cagaggcgct atgtgtatta ttatagctac ctgttaaaga
     1501 atcatctgga ttatagacca gtggcactgt tgtttcacaa gatgatgttt gaaactattc
     1561 caatgttcag tggcggaact tgcaatcctc agtttgtggt ctgccagcta aaggtgaaga
     1621 tatattcctc caattcagga cccacacgac gggaagacaa gttcatgtac tttgagttcc
     1681 ctcagccgtt acctgtgtgt ggtgatatca aagtagagtt cttccacaaa cagaacaaga
     1741 tgctaaaaaa ggacaaaatg tttcactttt gggtaaatac attcttcata ccaggaccag
     1801 aggaaacctc agaaaaagta gaaaatggaa gtctatgtga tcaagaaatc gatagcattt
     1861 gcagtataga gcgtgcagat aatgacaagg aatatctagt acttacttta acaaaaaatg
     1921 atcttgacaa agcaaataaa gacaaagcca accgatactt ttctccaaat tttaaggtga
     1981 agctgtactt cacaaaaaca gtagaggagc cgtcaaatcc agaggctagc agttcaactt
     2041 ctgtaacacc agatgttagt gacaatgaac ctgatcatta tagatattct gacaccactg
     2101 actctgatcc agagaatgaa ccttttgatg aagatcagca tacacaaatt acaaaagtct
     2161 gagaattcga tatctcgagt aattcgccag cacagtggtc gaccctgtgg aatgtgtgtc
     2221 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2281 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     2341 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     2401 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     2461 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     2521 ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc acggtgcgcc
     2581 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     2641 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     2701 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     2761 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     2821 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     2881 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     2941 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     3001 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     3061 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     3121 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     3181 ccgaaaggag cgcacgaccc catgcatcga taaaataaaa gattttattt agtctccaga
     3241 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     3301 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     3361 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     3421 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     3481 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     3541 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     3601 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     3661 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     3721 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     3781 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     3841 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg
     3901 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     3961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccgca aatcaatcta
     4861 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4921 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4981 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5041 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5101 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5161 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5221 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5281 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5341 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5401 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5461 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5521 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5581 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5641 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5701 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5761 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5821 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5881 tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     5941 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     6001 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     6061 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     6121 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     6181 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     6241 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     6301 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcat
//
