LOCUS       sequence-173678-        3705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression vector for producing 6x histidine tagged rat 
            DYRK1A kinase domain (residues 1-497).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3705)
  TITLE     DYRK1A assay plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHT-497
FEATURES             Location/Qualifiers
     source          1..3705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     promoter        complement(1510..1528)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1588..1606)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1674..1778
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1779..1847,1848..2639)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1997..2016)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2810..3398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3299..3318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3552..3569
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3650..3669
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3650..3668
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 catcatcatc atcatcacca tacaggagga gagacttcag catgcaaacc ttcatctgtc
       61 cggctcgcac cgtcattctc attccatgct gccggccttc agatggctgc acagatgccc
      121 cattcacatc agtacagtga ccgtcgccag ccaaacataa gtgaccagca ggtttctgcc
      181 ttatcatatt ctgaccagat tcagcaacct ctaactaacc agaggcggat gccccaaacc
      241 ttccgtgatc cagcaactgc tcctctgaga aaactctctg tggacttgat caaaacatac
      301 aagcatatta atgaggtcta ctatgcaaaa aagaagcgaa gacaccaaca gggccagggg
      361 gacgattcta gtcataagaa ggagcggaag gtttacaacg atggttacga tgacgataac
      421 tatgattaca ttgtaaaaaa cggagaaaag tggatggacc gttatgaaat cgactcctta
      481 ataggcaaag gttcatttgg acaggttgtg aaagcatatg acagagtgga acaagaatgg
      541 gtcgccatta aaatcatcaa gaacaagaag gcgtttctga atcaagcgca gatagaagtg
      601 cggctgcttg agctcatgaa caaacatgac actgagatga agtactacat agtgcatttg
      661 aaacgccact ttatgtttcg gaaccatctc tgtctagtgt ttgaaatgct gtcctataat
      721 ctctatgatt tgttgaggaa caccaacttc cgaggggtct ctttgaacct gacacgaaag
      781 tttgcacaac agatgtgcac agcattgctt ttccttgcga ctccagaact tagtatcatt
      841 cactgtgact taaagccgga gaacatcctt ctgtgtaacc ccaaacgaag tgcaatcaag
      901 attgttgact ttggcagctc ttgtcagttg gggcagagga tataccagta tattcagagt
      961 cgcttctatc ggtctccaga ggtgctactg ggaatgcctt atgaccttgc tatcgacatg
     1021 tggtcccttg gatgtatctt ggttgaaatg cacactggag agcctctgtt cagtggtgcc
     1081 aatgaggtgg atcagatgaa taaaatagtg gaagtcttgg gcatcccacc tgctcatatt
     1141 cttgaccaag caccgaaagc aagaaagttc tttgagaagt tgcctgatgg cacttggagc
     1201 ttaaagaaga ccaaagatgg aaaacgggag tacaaaccac caggaacccg taaacttcat
     1261 aatattcttg gagtggaaac aggaggacct ggcgggcggc gtgctgggga gtcaggtcac
     1321 actgtagctg actacttgaa gttcaaagac ctcattttaa ggatgcttga ttatgacccc
     1381 aaaactcgga ttcaacctta ttatgccctg cagcacagtt ttttcaagaa aacagctgat
     1441 gaaggtacaa acacaagcaa cagtgtgtcc accagccctt gactcgagag cttgcatcta
     1501 tggtctctcc ctttagtgag ggttaattag aaactggagt cgatgataag ctgtcaaaca
     1561 tgagaattaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     1621 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1681 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1741 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1801 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1861 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1921 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1981 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     2041 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2101 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2161 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2221 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2281 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2341 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2401 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2461 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2521 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2581 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2641 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2701 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2761 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2821 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2881 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2941 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3001 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3061 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3121 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3181 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3241 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3301 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3361 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3421 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3481 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3541 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3601 ctctccccgc gcgttggccg attcattaat gcagctggct tatcgaaatt aatacgactc
     3661 actataggga gaccggaatt caaaattaag gaggatccat cgatg
//
