LOCUS       sequence-17371-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  TITLE     HOX protein coding clones not referenced in publications
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HOXC5 (human) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             110..142
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tgggnnattc cctctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggctagcat
      121 gactggtgga cagcaaatgg gtcgggatcc gaattcgagc tcctacgtag ccaattcatt
      181 ctataagcag agccccaata tccctgccta taacatgcaa acttgtggga actatggatc
      241 ggcctcagag gtgcaggcat ccaggtactg ctacggcgga ttggacttaa gcatcacttt
      301 cccaccgcct gcgccttcca actctctcca cggggtagac atggctgcca acccccgggc
      361 tcaccccgac cgccccgcct gcagcgccgc ggccgctccg ggacacgctc cgggcagaga
      421 cgaagcggct cctctgaacc ccgggatgta cagtcagaag gcggctcgcc cggcgctgga
      481 ggagcgagct aagagcagtg gggagatcaa agaggagcag gcgcagacag ggcagcccgc
      541 cggactgagc cagccaccgg ccccgccaca gatttacccg tggatgacca aactgcacat
      601 gagccacgag acggacggca agcggtcccg aaccagttac acgcgctacc agactctgga
      661 actcgagaaa gaattccact ttaaccgcta cctcactcgc cgcaggcgca tagagatcgc
      721 caacaacttg tgtctcaatg agagacagat caagatcttc gagcaccacc accacnac
//
