LOCUS       sequence-17373-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     HOX protein coding clones not referenced in publications
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HOXC8 (murine) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(797..829)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(833..850)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(860..877)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgggtcct
       61 tattttcttc cttttcctcc tcctctttct cctcttcctc attcccttct tcttccacct
      121 tctcctcatc tcgggcccca ggcagtttat ccttgttgtt ctcctttttc cacttcatcc
      181 ctcgattctg gaaccaaatc ttcacttgtc tttctgtcag tcccagggcg tgagagactt
      241 caatccggcg ctttctggtc aaataaggat taaagagaaa ctccttctct agttccaagg
      301 tctgataccg gctgtaagtt tgtcgaccgc tgcgccgccc aggagcgtgg ggtctcatcc
      361 atggaaacat gaggctggga gacgagttct gatttaagtg gccttgtcct tcgctactgt
      421 tagtgttggc ggaggattta cagtcgggat attgcaccac gctcgcctct tgctgagccc
      481 cataaaggga ctgtctgggg agcgcctcgt agccatagaa tttggaggcg tctccgtggc
      541 agctcagcga gcatgggttc tgctggtagc ccgagttgga gatgccggag gtgccgtggt
      601 ggaagaagtc ttggacgtgg tgcgaggcgt gctggaaacc gggcgcggag ccgccgggcc
      661 cgtacaccag cgcatggctt ctgcccacgc tctgagggaa ccggcagtcg taataggccg
      721 gttccaggga ctcgccgcct ttgtatttgg aaaacagggg gttgacgaag taggagctca
      781 tggtcgcgga tccgcgaccc atttgctgtc caccagtcat gctagccata tggctgccgc
      841 gcggcaccag gccgctgctg tgatgatgat gatgatggct gctgcccatg g
//
