LOCUS       sequence-173744-        5760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of doubleTAL staple protein dT27 with N-terminally fused 
            cycle3-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pSB1A3_GFP_dT27
FEATURES             Location/Qualifiers
     source          1..5760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..714
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(31..59)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     654..674
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             5722..5739
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5740..5757
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt
      181 gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca tatgaaacgg
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
      301 aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct cggacacaaa
      421 ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
      481 atcaaagcta acttcaaaat tcgccacaac attgaagatg gatccgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaatgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caagtggggt
      721 cttagcgcgg gcgccgtaga tttgagaact ttgggatatt cacagcagca gcaggaaaag
      781 atcaagccca aagtgaggtc gacagtcgcg cagcatcacg aagcgctggt gggtcatggg
      841 tttacacatg cccacatcgt agccttgtcg cagcaccctg cagcccttgg cacggtcgcc
      901 gtcaagtacc aggacatgat tgcggcgttg ccggaagcca cacatgaggc gatcgtcggt
      961 gtggggaaac agtggagcgg agcccgagcg cttgaggccc tgttgacggt cgcgggagag
     1021 ctgagagggc ctccccttca gctggacacg ggccagttgc tgaagatcgc gaagcgggga
     1081 ggagtcacgg cggtcgaggc ggtgcacgcg tggcgcaatg cgctcacggg agcacccctc
     1141 aacctgaccc cagagcaggt cgtggcgatc gcaagccacg acggtggcaa acaggctctg
     1201 gaaacagtac agaggctgtt gcctgtgctg tgccaagcgc acgggttgac cccagagcag
     1261 gtcgtggcaa tcgcgagcaa taacggtggc aaacaggctc tggaaacggt gcagaggctc
     1321 cttccagtgc tgtgccaagc gcacggactt accccagagc aggtcgtggc cattgcctcg
     1381 aatggaggtg gcaaacaggc tctggaaacc gtacaacgat tgctgccggt gctgtgccaa
     1441 gcgcacggtt taaccccaga gcaggtcgtg gcaatcgcga gcaataacgg tggcaaacag
     1501 gctctggaaa cggtgcagag gctccttcca gtgctgtgcc aagcgcacgg gctcacccca
     1561 gagcaggtcg tggcaatcgc ctccaacatt ggtggcaaac aggctctgga aactgtccag
     1621 cgcctgcttc ccgtgctgtg ccaagcgcac ggtctgaccc cagagcaggt cgtggccatt
     1681 gcctcgaatg gaggtggcaa acaggctctg gaaaccgtac aacgattgct gccggtgctg
     1741 tgccaagcgc acgggttaac cccagagcag gtcgtggcaa tcgcctccaa cattggtggc
     1801 aaacaggctc tggaaactgt ccagcgcctg cttcccgtgc tgtgccaagc gcacggactc
     1861 accccagagc aggtcgtggc aatcgcctcc aacattggtg gcaaacaggc tctggaaact
     1921 gtccagcgcc tgcttcccgt gctgtgccaa gcgcacggtt tgaccccaga gcaggtcgtg
     1981 gccattgcct cgaatggagg tggcaaacag gctctggaaa ccgtacaacg attgctgccg
     2041 gtgctgtgcc aagcgcacgg gttgacccca gagcaggtcg tggccattgc ctcgaatgga
     2101 ggtggcaaac aggctctgga aaccgtacaa cgattgctgc cggtgctgtg ccaagcgcac
     2161 ggacttaccc cagagcaggt cgtggccatt gcctcgaatg gaggtggcaa acaggctctg
     2221 gaaaccgtac aacgattgct gccggtgctg tgccaagcgc acggactgac cccagagcag
     2281 gtcgtggcaa tcgcgagcaa taacggtggc aaacaggctc tggaaacggt gcagaggctc
     2341 cttccagtgc tgtgccaagc gcacggcctg accccagagc aggtcgtggc cattgcctcg
     2401 aatggaggtg gcaaacaggc tctggaaacc gtacaacgat tgctgccggt gctgtgccaa
     2461 gcgcacggtt taaccccaga gcaggtcgtg gcaatcgcga gcaataacgg tggcaaacag
     2521 gctctggaaa cggtgcagag gctccttcca gtgctgtgcc aagcgcacgg gctcacccca
     2581 gagcaggtcg tggcgatcgc aagccacgac ggtggcaaac aggctctgga aacagtacag
     2641 aggctgttgc ctgtgctgtg ccaagcgcac ggtctgaccc cagagcaggt cgtggcaatc
     2701 gcgagcaata acggtggcaa acaggctctg gaaacggtgc agaggctcct tccagtgctg
     2761 tgccaagcgc acgggttaac cccagagcag gtcgtggcaa tcgcctccaa cattggtggc
     2821 aaacaggctc tggaaactgt ccagcgcctg cttcccgtgc tgtgccaagc gcacggactc
     2881 accccagagc aggtcgtggc cattgcctcg aatggaggtg gcaaacaggc tctggaaacc
     2941 gtacaacgat tgctgccggt gctgtgccaa gcgcacgggt tgaccccaga gcaggtcgtg
     3001 gcgatcgcaa gccacgacgg tggcaaacag gctctggaaa cagtacagag gctgttgcct
     3061 gtgctgtgcc aagcgcacgg acttacccca gagcaggtcg tggccattgc ctcgaatgga
     3121 ggtggcaaac aggctctgga aaccgtacaa cgattgctgc cggtgctgtg ccaagcgcac
     3181 ggactgagcg cgggcgccgt agatttgaga actttgggat attcacagca gcagcaggaa
     3241 aagatcaagc ccaaagtgag gtcgacagtc gcgcagcatc acgaagcgct ggtgggtcat
     3301 gggtttacac atgcccacat cgtagccttg tcgcagcacc ctgcagccct tggcacggtc
     3361 gccgtcaagt accaggacat gattgcggcg ttgccggaag ccacacatga ggcgatcgtc
     3421 ggtgtgggga aacagtggag cggagcccga gcgcttgagg ccctgttgac ggtcgcggga
     3481 gagctgagag ggcctcccct tcagctggac acgggccagt tgctgaagat cgcgaagcgg
     3541 ggaggagtca cggcggtcga ggcggtgcac gcgtggcgca atgcgctcac gggagcaccc
     3601 ctcaacctga ccccagagca ggtcgtggca atcgcctcca acattggtgg caaacaggct
     3661 ctggaaactg tccagcgcct gcttcccgtg ctgtgccaag cgcacggttt aaccccagag
     3721 caggtcgtgg caatcgcgag caataacggt ggcaaacagg ctctggaaac ggtgcagagg
     3781 ctccttccag tgctgtgcca agcgcacggg ctcaccccag agcaggtcgt ggccattgcc
     3841 tcgaatggag gtggcaaaca ggctctggaa accgtacaac gattgctgcc ggtgctgtgc
     3901 caagcgcacg ggttaacccc agagcaggtc gtggccattg cctcgaatgg aggtggcaaa
     3961 caggctctgg aaaccgtaca acgattgctg ccggtgctgt gccaagcgca cgggttgacc
     4021 ccagagcagg tcgtggcgat cgcaagccac gacggtggca aacaggctct ggaaacagta
     4081 cagaggctgt tgcctgtgct gtgccaagcg cacggactta ccccagagca ggtcgtggcc
     4141 attgcctcga atggaggtgg caaacaggct ctggaaaccg tacaacgatt gctgccggtg
     4201 ctgtgccaag cgcacggact gaccccagag caggtcgtgg caatcgcctc caacattggt
     4261 ggcaaacagg ctctggaaac tgtccagcgc ctgcttcccg tgctgtgcca agcgcacggc
     4321 ctgaccccag agcaggtcgt ggcgatcgca agccacgacg gtggcaaaca ggctctggaa
     4381 acagtacaga ggctgttgcc tgtgctgtgc caagcgcacg gtttaacccc agagcaggtc
     4441 gtggcaatcg cctccaacat tggtggcaaa caggctctgg aaactgtcca gcgcctgctt
     4501 cccgtgctgt gccaagcgca cgggctcacc ccagagcagg tcgtggccat tgcctcgaat
     4561 ggaggtggca aacaggctct ggaaaccgta caacgattgc tgccggtgct gtgccaagcg
     4621 cacggcctaa ccccagagca ggtcgtggca atcgcgagca ataacggtgg caaacaggct
     4681 ctggaaacgg tgcagaggct ccttccagtg ctgtgccaag cgcacggtct gaccccagag
     4741 caggtcgtgg ccattgcctc gaatggaggt ggcaaacagg ctctggaaac cgtacaacga
     4801 ttgctgccgg tgctgtgcca agcgcacggg ttaaccccag agcaggtcgt ggcaatcgcg
     4861 agcaataacg gtggcaaaca ggctctggaa acggtgcaga ggctccttcc agtgctgtgc
     4921 caagcgcacg gactcacccc agagcaggtc gtggcgatcg caagccacga cggtggcaaa
     4981 caggctctgg aaacagtaca gaggctgttg cctgtgctgt gccaagcgca cgggttgacc
     5041 ccagagcagg tcgtggccat tgcctcgaat ggaggtggca aacaggctct ggaaaccgta
     5101 caacgattgc tgccggtgct gtgccaagcg cacggactta ccccagagca ggtcgtggca
     5161 atcgcgagca ataacggtgg caaacaggct ctggaaacgg tgcagaggct ccttccagtg
     5221 ctgtgccaag cgcacggact gaccccagag caggtcgtgg caatcgcgag caataacggt
     5281 ggcaaacagg ctctggaaac ggtgcagagg ctccttccag tgctgtgcca agcgcacggc
     5341 ctgaccccag agcaggtcgt ggccattgcc tcgaatggag gtggcaaaca ggctctggaa
     5401 accgtacaac gattgctgcc ggtgctgtgc caagcgcacg gtttaacccc agagcaggtc
     5461 gtggcaatcg cctccaacat tggtggcaaa caggctctgg aaactgtcca gcgcctgctt
     5521 cccgtgctgt gccaagcgca cgggctcacc ccagagcagg tcgtggcaat cgcctccaac
     5581 attggtggca aacaggctct ggaatctatc gttgctcagc tgtcacgtcc ggacccggct
     5641 ctggctgctc tgaccaacga ccacctggtt gctctggctt gcctgggtgg tcgtccggct
     5701 atggacgctg ttaaaaaagg cctagtgccg cgcggcagcc accaccatca ccatcattaa
//
