LOCUS       sequence-174075-        5453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Setd7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Kofent J, Zhang J, Spagnoli FM
  TITLE     The histone methyltransferase Setd7 promotes pancreatic progenitor 
            identity.
  JOURNAL   Development. 2016 Oct 1;143(19):3573-3581. Epub 2016 Aug 30.
  PUBMED    27578787
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-1"; volume: "143(19)"; pages: 
            "3573-3581. Epub 2016 Aug 30"
COMMENT     Sequence Label: m.Setd7
FEATURES             Location/Qualifiers
     source          1..5453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             69..98
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             108..137
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             147..176
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             186..215
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             225..254
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             288..317
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1471..1605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1495..1514)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1549..1568
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1718..1738)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1718..1736)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1757..1773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1757..1773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1770..1792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1781..1797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1805..1811,1812..1829,1830..1835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1850..1871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1988..2005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2159..2747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2239..2258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2918..3709,3710..3778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3541..3560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3779..3883)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3909..4364)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4046..4067)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4258..4277
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4435..5419
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 atttaggtga cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattta
       61 aagctatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca
      121 tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg
      181 aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt
      241 ctgaagagga cttgaatgaa atggagagct tgggcgacct caccatggag caaaagctca
      301 tttctgaaga ggacttgaat tccatggata gcgacgatga ggtggtggag gaagccgtgg
      361 aagggcacct ggacgatgac ggattaccac acgggttctg cactgtcacc tactcctcca
      421 cggacagatt tgagggcaac tttgtgcatg gagaaaagaa tgggcgtggg aaattcttct
      481 tctttgacgg cagcaccctg gagggatatt acgtggacga tgccctgcag ggccagggcg
      541 tttacaccta tgtggatgga ggtgttctcc aaggcaccta tgtggatgga gagctgaatg
      601 gaccggccca ggagtacgac tctgacggga ggctgatctt caagggacag tacaaagaca
      661 acaatcgaca cggagtgtgt tggatccatt acccggatgg aggcagcctc gtcggggaag
      721 tcaatgaaga tggagagatg acgggagaaa agatcgccta tgtgtaccct gaccagagga
      781 ctgcgctcta tgggaagttc attgacggag agatgctcga agggaaactg gccaccctca
      841 tggccaccga agaagggagg ccacactttg aagtgacgtc gggaagttcc gtgtaccact
      901 ttgataaatc gacttcctcc tgcatctcca gcgatgctct ccttccagac ccttatgagt
      961 ctgagagggt ttatgttgcc gactctctca tctccagtgc tggagaaggg ctgttttcca
     1021 aggtagcagt tggacctaat actgttatgt cgttttataa tggagtccga attacacacc
     1081 aagaggttga cagcagggat tgggccctaa atgggaacac tctctccctg gacgaggaga
     1141 cagtcattga tgtgcccgag ccctacaacc acgtgtccaa gtactgtgcc tccttgggac
     1201 acaaggccaa tcactccttc actccgaact gcgtctatga cctgtttgtc cacccccgtt
     1261 tcgggccgat caagtgcatc cgcaccctgc gagctgtgga ggccgaggaa gagctgaccg
     1321 ttgcctacgg ctatgaccac agccccccgg ggaagagtgg gcctgaagcc cccgagtggt
     1381 atcaagtgga gctgaaggcc ttccaggcca cccagcagaa gaattcaagg cctctcgagc
     1441 ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     1501 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1561 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1621 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcg
     1681 gcgccaatgc attgggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
     1741 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1801 cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1861 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1921 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2041 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2101 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2161 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2221 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2281 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2341 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2401 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2461 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2521 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2581 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3901 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     3961 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4021 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4081 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4141 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4201 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4261 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4321 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     4381 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagtcgac
     4441 cgccaattca atatggcgta tatggactca tgccaattca atatggtgga tctggacctg
     4501 tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc
     4561 agccaattca atatggcgga cttggcacca tgccaattca atatggcgga cctggcactg
     4621 tgccaactgg ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct
     4681 gtgccaagtc cgccatattg aattggcatg gtgccaataa tggcggccat attggctata
     4741 tgccaggatc aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc
     4801 aggttcaata ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat
     4861 tgacgtagat agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata
     4921 ccgcccatag ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga
     4981 cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt
     5041 aaatggcccg cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca
     5101 atgggatggc tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg
     5161 gtaaatggcc cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta
     5221 ctattggaag tacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat
     5281 ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca
     5341 aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag
     5401 caatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttg
//
