LOCUS       sequence-17417-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  TITLE     pPS
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPS293
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..30
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14..30
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(42..483)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(48..71)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    366..483
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 cctgcaggtc gactctagaa ctagtggatc cggggttttt tctccttgac gttaaagtat
       61 agaggtatat taacaatttt ttgttgatac ttttattaca tttgaataag aagtaataca
      121 aaccgaaaat gttgaaagta ttagttaaag tggttatgca gtttttgcat ttatatatct
      181 gttaatagat caaaaatcat cgcttcgctg attaattacc ccagaaataa ggctaaaaaa
      241 ctaatcgcat tatcatccta tggttgttaa tttgattcgt tcatttgaag gtttgtgggg
      301 ccaggttact gccaattttt cctcttcata accataaaag ctagtattgt agaatcttta
      361 ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac
      421 gaggacgcac ggaggagagt cttccttcgg agggctgtca cccgctcggc ggcttctaat
      481 ccgtacttca atatagcaat gagcagttaa gcgtattact gaaagttcca aagagaaggt
      541 ttttttaggc taagataatg gggctcttta catttccaca acatataagt aagattagat
      601 atggatatgt atatggatat gtatatggtg gtaatgccat gtaatatgat tattaaactt
      661 ctttgcg
//
