LOCUS       sequence-174176-       10168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Outer mitochondrial membrane-targeted mCherry-tagged recruiter 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I
  TITLE     Localization of mTORC2 activity inside cells.
  JOURNAL   J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 
            10.1083/jcb.201610060.
  PUBMED    28143890
REFERENCE   2  (bases 1 to 10168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060."
COMMENT     Sequence Label: pHR-FKBP:mCherry-BclXLc33
FEATURES             Location/Qualifiers
     source          1..10168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     138..155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(225..245)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        230..559
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      410..545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1693..1758
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1888..1908
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2333..2467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2370..2389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2424..2443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2635..3268
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3315..3440
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3937..4170
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4355..4399
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4684..4801
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4954..5361
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      5395..5404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5401..5403,5404..5724)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             5749..6456
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5894..5912)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6162..6181
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      6466..6475
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6478..6504
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6666..7254
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6719..6739)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7137..7148)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7257..7273)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7258..7274)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7364..7597
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7622..7640)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7623..7640)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7930..7949)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8163..8182)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8282..8304
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8342..8360)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8428..8532
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8533..8601,8602..9393)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8751..8770)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9564..10152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10053..10072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
       61 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      121 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      181 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
      241 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      301 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      361 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      421 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      481 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      541 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
      601 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
      661 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
      721 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
      781 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
      841 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
      901 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
      961 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     1021 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     1081 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     1141 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     1201 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     1261 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     1321 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     1381 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     1441 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
     1501 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     1561 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
     1621 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     1681 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     1741 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     1801 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     1861 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     1921 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     1981 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     2041 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     2101 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     2161 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     2221 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2281 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2341 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2401 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2461 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     2521 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     2581 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     2641 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     2701 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     2761 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     2821 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     2881 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     2941 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     3001 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     3061 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     3121 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3181 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3241 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     3301 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     3361 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     3421 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     3481 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     3541 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     3601 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     3661 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     3721 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     3781 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     3841 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     3901 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     3961 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     4021 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     4081 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     4141 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     4201 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     4261 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     4321 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     4381 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     4441 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     4501 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     4561 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     4621 cagatccatt cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca
     4681 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     4741 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     4801 tcgggtttat tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt
     4861 cctgcagccc cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     4921 gaccccacct gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa
     4981 aataccaaac caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg
     5041 ttgggccaaa caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag
     5101 atggtcaccg cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc
     5161 ccaaccctca gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga
     5221 aatgaccctg cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg
     5281 cttctgcttc ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc
     5341 cgacagactg agtcgcccgg gggggatctg gagctctcga gaattctcac gcgtgccacc
     5401 atgggagtgc aggtggagac tatctcccca ggagacgggc gcaccttccc caagcgcggc
     5461 cagacctgcg tggtgcacta caccgggatg cttgaagatg gaaagaaatt tgattcctcc
     5521 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg
     5581 gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat
     5641 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc
     5701 gatgtggagc ttctaaaact ggaaggtgct gcacttctgc ccggttctat ggtgagcaag
     5761 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     5821 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     5881 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     5941 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     6001 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6061 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     6121 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     6181 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     6241 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     6301 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     6361 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     6421 cgccactcca ccggcggcat ggacgagctg tacaagcccg gttctgccgc catggcatat
     6481 ccatatgacg tcccagacta tgctgccgag agccgaaagg gccaggaacg cttcaaccgc
     6541 tggttcctga cgggcatgac tgtggccggc gtggttctgc tgggctcact cttcagtcgg
     6601 aaatgagcgg ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta
     6661 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6721 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6781 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6841 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6901 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6961 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7021 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7081 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7141 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7201 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     7261 accgtcgacc tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag
     7321 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7381 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7441 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7501 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7561 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     7621 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     7681 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     7741 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     7801 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     7861 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     7921 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     7981 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     8041 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     8101 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     8161 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     8221 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     8281 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     8341 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8401 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8461 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8521 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8581 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8641 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8701 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8761 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8821 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8881 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8941 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9001 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9061 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9121 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9181 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9241 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9301 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9361 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9421 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9481 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9541 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9601 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9661 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9721 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9781 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9841 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9901 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9961 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10021 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10081 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10141 agcctatgga aaaacgccag caacgcgg
//
