LOCUS       sequence-174226-        9553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses his-tagged C151S human Keap1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y
  TITLE     Substoichiometric Hydroxynonenylation of a Single Protein 
            Recapitulates Whole-Cell-Stimulated Antioxidant Response.
  JOURNAL   J Am Chem Soc. 2014 Dec 31.
  PUBMED    25544059
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2014 Dec 31."
COMMENT     Sequence Label: pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S
FEATURES             Location/Qualifiers
     source          1..9553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     445..464
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(561..877)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      575..710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(917..939)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1039..1058
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1266..1283
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1267..1283
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3887..3907)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(3914..4804)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             complement(4808..4825)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(4835..4852)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(4865..4873,4874..4876,4877..5451)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5038..5057)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5267..5284
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5494..6171)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(5556..5579)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5754..5773)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5971..5991
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6023..6041
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(6196..6399)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6225..6245)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6400..6703
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(6824..6843)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6825..6843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6855..6873)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6856..6873)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6893..6909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6893..6909)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6906..6928)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6917..6933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6941..6947,6948..6965,6966..6971))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6986..7007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7124..7141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7295..7883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7375..7394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8054..8845,8846..8914))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8677..8696
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8915..9019)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9107..9188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9132..9151
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(9418..9553,1..464))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9501..9521)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc
       61 tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc
      121 ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc
      181 atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag
      241 accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc
      301 gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg gtcgacggtg
      361 tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc ccgggggacg
      421 tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggaagg tcgtctcctt
      481 gtgaggggtc aggggcgtgg gtcaggggat ggtggcggca ccggtcgtgg cggccgacct
      541 gcaggcatgc aagctagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
      601 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
      661 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
      721 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
      781 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
      841 cttctgcctg ctggggagcc tggggacttt ccacaccaat ctttcgtctc gcgcgtttcg
      901 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
      961 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     1021 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     1081 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     1141 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     1201 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagacac
     1261 gacgttgtaa aacgacggcc agtgccaagc tagcggccgc atacaaaaaa ccaacacaca
     1321 gatccaatga aaataaaaga tcctttatta agcttagctg aaaacttctg gctcacatag
     1381 cttctgtcac caccaatgag atgccacatg cgctgactac ttgggtcact tttaattttt
     1441 atgttgctga ttgtatgtgt gcatctcctg tcttgtaagc attaaacaaa tgaagacata
     1501 tgcagagctg tgaaattcac tctgcactga atagacaaaa agggttacat agcataacaa
     1561 tgagcagttg gtatccacgt ggttctcagc attataaact ctggggaaga tgctacttcg
     1621 taacctcaag gaaataagta agcaaggcag aaggaacact taaacaagta ttattcaaca
     1681 gacatgttta cactaaataa ttaaggttat atttagggtt taaactcatc cctgaatatc
     1741 cttagtaaca acctggagga aaaactgggg tgaatgagaa cttaagtttt ccaaggatta
     1801 tggttcaaat cctctgaaat ctataacagg ttaaaaaaat gagaactcaa ctttctagag
     1861 caataactct tctcgagggc actagcacaa ggcggtagtg attttcactt ttaatatatt
     1921 acatataata ctacaaaaag actgaagttt taaggaaaat ccattattat taaaagttta
     1981 gtactcttga gtctggactt tctgatcaac aggtacagtt ctgctggtca atctgcttcc
     2041 ggcagggctc catggtgaca gccacgccca ccccactccg gcccgatgtc attcgggtca
     2101 cctcgctcca ggtgtctgta tctgggtcgt aacactccac actgtccagg aacgtgtgac
     2161 catcatagcc tccaaggacg tagattctcc cctggtggac agtgatcccc agggcacttc
     2221 gccggtgctt catgggggct acgaaagtcc acgtctctgt ttccacatcg tagcgctcca
     2281 cgctgttcag ctggtcctga ccatcatagc ccccagcagc atagatacag ttgtgcagga
     2341 cgcagacgcc tgccccgctt cggatggtgt tcattgctgt gatcattcgc cactcgttcc
     2401 tctctgggta gtaacactca gctgaattaa ggcggtttgt cccgtcaaag ccccccacgg
     2461 cataaaggag acgattgagg acagccacgc ccaccccgat ccttcgtgtc agcattgggg
     2521 ccaccaagtg ccactcatcc cgctctggct catacctctc cacactgttg tggtggatgc
     2581 agccgtggga gccgccgacg gcatagatgt ggccatcgat gacccccacc ccgatgcggt
     2641 tacggggcac gctcatgggg gcgcagggcg accactgatt ggtcatgggg ttgtaacagt
     2701 ccagggcgct ggagtcggtg ttgccgtcgg gcgagttgtt cctgccgccc acggcgtaca
     2761 acagcccgcc caccacgcag ccggccaggc cgctccgcgg cacctgcagg tccgccaacc
     2821 ggagccaggt gccgtcactg gggttgtaag cctccaggta gctgagcgac tgtcggaagt
     2881 agccgcccgc ggtgtagatc aggcggccca ccttgggcgc ccggcagggc atcacctgcg
     2941 tgggcttgtg cagggtgagc tcctcgaaga tcttgaccag gtagtccttg cagcgggagt
     3001 cggactgcag gatctcgcac ttctgcagct gcatctgcag gaagttcggc gtcaacgagt
     3061 ggcagcgcac tgcccgcagc agcgcctgga cgtagaaccg tcgctgttcg cagtcgtact
     3121 tgacccagtt gatgcaggcg tggaagacct cggactcgca gcgcacgttc aggtcgtccc
     3181 ggctgatgag ggtcaccagt tggcagtggg acaggttgaa gaactcctct tgcttggcca
     3241 cctccccaaa atgcatgtag atgtactccc gggcacgctg gtgcaactcc acacagccaa
     3301 tctgctcagc gaagttggcg atgccgatgg cattgctggg gtccagctgc tgcaccagga
     3361 agtcactgca ggcacggaca acgctgtcga tctggtacat gacagcaccg ttcatgacgt
     3421 ggaggacact cttctcgccc atggagatgg aggccgtgta ggcgaattca atgaggcgct
     3481 ccatgacctt ggggtggata ccctcaatgg acaccacctc catgccctgc tcccgcagcc
     3541 cgttggtgaa catggccttg aagacagggc tggatgaggc cagcaccacc ttgtgggcca
     3601 tgaactgggc ggccggtgca tcctggtact tgacctgcag tgtgacgtca cacagctgct
     3661 ggctgagccg cagctcgttc atgatgccaa aggcctgctt ggtatgatcc tccagggtgt
     3721 agctgaaggt gcggttgcca tgctgggagg gcgtcacctc cgccttgcac tcagtggagg
     3781 cgtacatcac cgcgtcccct gccccctcag ggcactgtga ctgcaggggc aggaatcggc
     3841 agcaggcccc agccccgcta ggcctgggat ctggctgcat ccctgagccc tggaaataca
     3901 agttttctcc ggagccggaa atctcgagcg tcgacagcca gcgcgcgatc tcgctgccga
     3961 tcaggtccgg gttgtcttct tgcagcagat tcagacccgg gccgatgtcc acagccttgc
     4021 agttaggcag gcttttggcc aggcgagcgg cttcggccgg tgggatcaga acgcctgggg
     4081 tgccccagaa cagcagcttc gggacagggg actggtgcag ccagtccatg tattcttcga
     4141 ccagcgcgac gatgttcgct ggctcaccgg cgattggcag ctcgtttggg aagcgccaca
     4201 gtggctcgcg gtcaacaggg ttcaggaacg gctcgcggta atggtccatc tcgacttcag
     4261 tcagcgggcg gacgacaccc atcggcagcg taccctcgat aaaaacgttc tgatcgatga
     4321 tcagcttgcg gccgacgtcg gtggtgcgga aggcctggaa ggtctcgcgg gcaaattctg
     4381 gccattcgtc ccaggtcggg atagggcgga tgaactccat aaatgcaata cctttgacgc
     4441 gctctggatt gcgcttggcc cagtggaaac ccagagcgga gccccagtcg tgaatgacca
     4501 ggacgacctc ttccagaccc agggcttcga tgaaggcatc catgaagcgg acgtggtcgt
     4561 cgaagaaata acccaggtct ggtttgtcgg atttgcccat accgatcagg tctggagcaa
     4621 tgcagcgatg ggtcggtgca acatgcggga tgatgttgcg ccacacgtag gaggaggtcg
     4681 ggttaccgtg caggaacagc acaggggtgc catcgcgcgg accaacatcg acgtagtgca
     4741 tgcgctcgcc caggacttcc acataatggg ggtcgaatgg aaagccagta ccgatttctg
     4801 ccatatggct accacgaggc accaggccgc tgctgtgatg atgatgatga tggctgctgc
     4861 ccatggttgt ggccatatta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc
     4921 ggggggccta gacgtttttt taacctcgac taaacacatg taaagcatgt gtaccgaggc
     4981 cccagatcag atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat
     5041 acgcttgagg agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg
     5101 ggttgtgccg cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg
     5161 tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa
     5221 gaagcttcca gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg
     5281 agaggggaaa gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc
     5341 acattgccaa aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct
     5401 tattccaagc ggcttcggcc agtaacgtta gggggggggg agggagaggg gcggatcccg
     5461 ggcccgcggt accgtcgact gcagaattgg ccgctacagg aacaggtggt ggcggccctc
     5521 ggcgcgctcg tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
     5581 ggagtccacg tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt
     5641 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt
     5701 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tagtcttctt
     5761 ctgcattacg gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa
     5821 ggagccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
     5881 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc
     5941 ggcggggtgc ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca
     6001 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     6061 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
     6121 gcgcaccttg aagcgcatga actccttgat gacgtcctcg gaggaggcca tggtggatcc
     6181 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     6241 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     6301 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     6361 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     6421 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     6481 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     6541 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     6601 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     6661 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     6721 gctatgaact aatgaccccg taattgatta ctattaataa ctaagatctg gtaccttgaa
     6781 ttcatgcttc tcctcccttt agtgagggta attctctctc tctccctata gtgagtcgta
     6841 ttaattcctt ctcttctata gtgtcaccta aatcgttgca attcgtaatc atgtcatagc
     6901 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6961 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7021 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7081 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7141 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7201 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7261 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7321 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7741 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7801 gatccggcaa aaaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7921 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8041 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8101 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8161 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8221 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8281 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8341 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8401 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8461 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8521 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8581 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8641 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8701 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8761 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8821 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8881 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8941 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9001 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9061 ttattatcat gacattaacc tataaaaata ggcgtatcac gagattgcag tgaaaaaaat
     9121 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     9181 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     9241 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag
     9301 agtcggtggg cctcgggggc gggtgcgggg tcggcggggc cgccccgggt ggcttcggtc
     9361 ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca
     9421 ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc
     9481 ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag
     9541 gaaggcgggc acc
//
