LOCUS       sequence-174298-        9157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged human PTP-SL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pLX302-PTP-SL-V5 puro
FEATURES             Location/Qualifiers
     source          1..9157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      13..601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     502..521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     755..772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    889..910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(925..930,931..948,949..955)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    963..979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     968..990
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     987..1003
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     987..1003
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1022..1042
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1024..1042
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1070..1296
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1297..1477
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1524..1649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2142..2375
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2560..2604
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2779..3279
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3198..3216
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3301..3900
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3301..3320)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3797..3817
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3942..4059
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4176..4479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4480..4683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4634..4654
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4706..4730
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5970..5994)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5996..6037
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6079..6667
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6132..6152)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6550..6561)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6739..6972
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7050..7171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7087..7106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7141..7160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7211..7346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7366..7385)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7367..7385)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7395..7412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7395..7411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7404..7426)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7553..8008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7640..7659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7850..7871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8034..8138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8139..8207,8208..8999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8357..8376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
       61 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      121 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      241 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      301 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      361 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      421 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      481 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      541 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      601 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      661 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      721 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      781 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      841 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      901 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      961 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     1021 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     1081 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     1141 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     1201 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     1261 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     1321 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     1381 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     1441 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     1501 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     1561 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1621 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1681 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1741 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1801 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1861 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1921 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1981 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     2041 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     2101 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     2161 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     2221 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     2281 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     2341 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     2401 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     2461 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     2521 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     2581 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     2641 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2701 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2761 ccaaccccga ggggaccctt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     2821 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
     2881 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     2941 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     3001 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     3061 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     3121 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     3181 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     3241 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggtaccacc ggagcttacc
     3301 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     3361 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     3421 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     3481 atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag
     3541 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     3601 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     3661 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     3721 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     3781 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     3841 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     3901 gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     3961 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     4021 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     4081 agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta atcaattacg
     4141 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     4201 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     4261 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     4321 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     4381 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     4441 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     4501 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     4561 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     4621 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     4681 gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagttggca ccatgattct
     4741 tcacagatta aaagaaagat ttcagctttc cttaagacaa gacaaagaga aaaaccagga
     4801 gatccaccta tcgcccatca cattacagcc agcactgtcc gaggcaaaga cagtccacag
     4861 catggtccaa cctgagcagg ccccaaaggt actgaatgtt gtcgtggacc ctcaaggccg
     4921 aggtgctcct gagatcaaag ctaccaccgc tacctctgtt tgcccttctc ctttcaaaat
     4981 gaagcccata ggacttcaaa agagaagagg gtccaacgta tctcttacat tggacatgag
     5041 tagcttgggg aacattgaac cctttgtgtc tataccaaca ccacgggaga aggtagcaat
     5101 ggagtatctg cagtcagcca gccgaattct cacaaggtct cagctgaggg acgtcgtggc
     5161 aagttcacat ttactccaaa gtgaattcat ggaaatacca atgaactttg tggatcccaa
     5221 agaaattgat attccgcgtc atggaactaa aaatcgctat aagaccattt taccaaatcc
     5281 cctcagcaga gtgtgtttaa gaccaaaaaa tgtaaccgat tcattgagca cctacattaa
     5341 tgctaattat attaggggct acagtggcaa ggagaaagcc ttcattgcca cgcagggccc
     5401 catgatcaac accgtggatg atttctggca gatggtttgg caggaagaca gccctgtgat
     5461 tgttatgatc acaaaactca aagaaaaaaa tgagaaatgt gtgctatact ggccggaaaa
     5521 gagagggata tatggaaaag ttgaggttct ggttatcagt gtaaatgaat gtgataacta
     5581 caccattcga aaccttgtct taaagcaagg aagccacacc caacatgtga agcattactg
     5641 gtacacctca tggcctgatc acaagactcc agacagtgcc cagcccctcc tacagctcat
     5701 gctggatgta gaagaagaca gacttgcttc ccagggccga gggcctgtgg ttgtccactg
     5761 cagtgcagga ataggtagaa cagggtgttt tattgctaca tccattggct gtcaacagct
     5821 gaaagaagaa ggagttgtgg atgcactaag cattgtctgc cagcttcgta tggatagagg
     5881 tggaatggtg caaaccagtg agcagtatga atttgtgcac catgctctgt gcctgtatga
     5941 gagcagactt tcagcagaga ctgtccagtt gccaactttc ttgtacaaag tggttggtaa
     6001 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg
     6061 gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6121 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6181 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6241 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6301 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6361 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6421 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6481 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6541 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6601 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6661 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6721 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     6781 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6841 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6901 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6961 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     7021 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7081 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7141 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7201 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7261 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7321 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7381 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7441 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7501 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7561 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7621 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7681 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7741 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7801 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7861 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7921 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7981 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8041 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8101 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8161 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8221 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8281 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8341 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8401 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8461 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8521 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8581 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8641 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8701 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8761 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8821 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8881 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8941 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9001 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9121 tttcgttcca ctgagcgtca gaccccgtag aaaagat
//
