LOCUS       sequence-174334-        5712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hybrid NarQ[1-217]-Tar[257-553] expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
  TITLE     Engineering Hybrid Chemotaxis Receptors in Bacteria.
  JOURNAL   ACS Synth Biol. 2016 Jun 10.
  PUBMED    27285081
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 10."
COMMENT     Sequence Label: pSB11
FEATURES             Location/Qualifiers
     source          1..5712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      462..496
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(915..1574)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1489..1508
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1575..1677)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2069..2086)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2203..2748)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2283..2302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtctcacgc gttgataccg gttcggtgct ggtcgaaagc gccggggaaa caatgaacaa
       61 tatcgtcaat gctgtcactc gcgtgactga cattatgggc gagattgcat cggcatcgga
      121 tgaacagagc cgtggcatcg atcaagtcgc attggcggtt tcggaaatgg atcgcgtcac
      181 gcaacagaac gcatcgctgg tgcaggaatc agctgccgcc gccgctgcgc tggaagaaca
      241 ggcgagtcgt ttaacgcaag cagtttccgc gttccgtctg gcagccagcc cactcaccaa
      301 taaaccgcaa acaccatccc gtcctgccag tgagcaacca ccggctcagc cacgactgcg
      361 aattgctgaa caagatccaa actgggaaac attttgagga tccgcggata aataagtaac
      421 gatccggtcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      481 gccgggcgtt ttttattggt gagaatcgca gcaacttgtc gcgccaatcg agccatgtcg
      541 tcgtcaacga ccccccattc aagaacagca agcagcattg agaactttgg aatccagtcc
      601 ctcttccacc tgctgaccgg atcagcagtc cccggaacat cgtagctgac gccttcgcgt
      661 tgctcagttg tccaaccccg gaaacgggaa aaagcaagtt ttccccgctc ccggcgtttc
      721 aataactgaa aaccatacta tttcacagtt taaatcacat taaacgacag taatccccgt
      781 tgatttgtgc gccaacacag atccgtcgac cgggtcgaat ttgctttcga atttctgcca
      841 ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa
      901 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      961 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1021 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1081 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     1141 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1201 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1261 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1321 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     1381 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1441 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1501 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1561 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1621 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     1681 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt
     1741 atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt
     1801 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
     1861 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
     1921 caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag
     1981 ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga
     2041 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     2101 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     2161 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     2221 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     2281 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     2341 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     2401 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     2461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     2521 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     2581 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     2641 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     2701 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
     2761 attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct
     2821 gaagtcagcc ccatacgata taagttgtaa ttctcatgtt tgacagctta tcatcgatcc
     2881 cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg
     2941 gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag
     3001 caatttaact gtgataaact accgcattaa agcttcttgc gaaacagctc tcgaccaggt
     3061 ttatactggt cgtgcagatg aaagcagttt ttgccgaggt cgattccgac cagcgccata
     3121 tcgctcatag tgatggtctc cgaattataa acaccctgcg aaagcgtagc ctacgcaggg
     3181 tgtcgggtgc tcatcccatt acccgttttc ccatctctta acttaagggg ggaacggtgt
     3241 cctgtctttc atggggctcg ttcactgcta acttacctgc ttccctgtct gtcaattctc
     3301 tctatcctgc gatcccgcga agaaccaaaa aagctcgaca gagggcgcgg tcattttagg
     3361 tcgggcggat cggcgccgcc ggctcggctg gtgtgccgca cagcaccgcc tacgtgagct
     3421 gccagttgat gaacttcccc cgttgccagc tagggcgcaa gcgggctgta taagatcact
     3481 gcccatcaca ttgatcggct cggatttttt ctcaatccgt aaacaggtca aacatcagtt
     3541 gccgcaacca aatattggct aggtccttgt ggtacttcgc atgccagaac atgttgatgg
     3601 ctatttcagg caagacgact gggtgcggca aggcgcttag gccgaagggc tccacacagc
     3661 agtcggctaa acgtatcggc acagtggcga gcagatcggt gcgctggagg atgtggccaa
     3721 cggcggcgaa gtgcggcact tccagacgga tgtcgcgccg gatgccgacc cgtgtcatgt
     3781 acgtgtccac ctcgccgtgg ccggtgccag cggcgatgac acgcacgtgg ccgtaggaac
     3841 agaagcgctc cagagtcagg ggttcgcggg tgactggatg gtccttgcga cataggcaca
     3901 cgtagtgatt ctggagcagc cggcgctgaa agaagccagt ttgcagattg ggaagcaggc
     3961 ccacggccaa gtccacggtt ccgttctgca aggcctgcat caggctcatc gaactgtcgc
     4021 gcaccgtact gatcacgcaa ttgggggcct ggtgagccag cacatccatc agccgcggca
     4081 tgaagtagat ctcgccaatg tcggtcatgg ccagggtgaa ggtacgctcg ctggtcagcg
     4141 gatcgaagct ttcatggtgc tgtagggcgt tgcgcagtgc gtgcatggcc gaagtgacgg
     4201 gctcggccag atgcgcggca tagggtgtgg gttccattcc ctgatgtgtg cgcacgaaga
     4261 gtgggtcctg tagcgaggtg cgcaggcgtt tcagcgcatt gctcacggca ggctgggtca
     4321 ggcccaggtt ctccgcagtg atagagacgc gtctgtcgac cagcaactgg ttgaacacca
     4381 ccagcaggtt taaatccagg tcacgcagtt ccatggggcc tcgcttgggt tattgctggt
     4441 gcccggccgg gcgcaatatt catgttgatg atttattata tatcgagtgg tgtatttatc
     4501 aatattgttt gctccgttat cgttattaac aagtcatcaa taatgccata aggagtacca
     4561 tatggtgatt gttaaacgac ccgtctcggc cagtctggcc cgggcctttt tttacattgt
     4621 gctgctgtcg attctttcca cgggtatcgc tctgctaact ctggcgagca gtttgcgcga
     4681 cgctgaggct atcaatattg ccggatcgct gcgtatgcag agttaccgcc tgggctacga
     4741 cttgcaaagt ggcagtccac aactcaatgc acatcgccag ttatttcagc aggcactgca
     4801 ttcaccggta ttaaccaacc tcaacgtctg gtatgtgcca gaagcagtaa aaactcgcta
     4861 tgcgcatctg aatgccaact ggctggagat gaataatcgg ctcagcaagg gcgatttgcc
     4921 gtggtatcag gccaatatta ataattatgt taatcagata gacctgttcg tactggcttt
     4981 acagcactac gctgaacgca aaatgctgct ggtggtggcg atttccctgg ctggcggcat
     5041 cggtattttc acgctggtct tttttacttt gcgccgcata cgccatcagg tggttgcccc
     5101 gctgaatcag ctggttaccg ccagtcagcg tattgaacac gggcagttcg actcgccgcc
     5161 gctggatacc aacctgccga atgagcttgg tctgcttgca aaaaccttta accagatgca
     5221 acgctctttg actgacaccg tcactcatgt ccgcgaaggt tcagatgcca tctatgccgg
     5281 tacccgtgaa attgcggcgg gcaacaccga tctttcctcc cgtactgaac agcaggcatc
     5341 cgcgctggaa gaaactgccg ccagcatgga gcagctcacc gcgacagtga agcaaaacgc
     5401 cgataacgcc cgccaggcct cgcaactggc gcaaagtgcc tccgacaccg cccagcacgg
     5461 cggcaaagtg gtggatggcg tagtgaaaac gatgcatgag atcgccgata gttcgaagaa
     5521 aattgccgac attatcagcg ttatcgacgg tattgccttc cagactaata tcctcgcgct
     5581 gaatgccgcg gttgaagccg cgcgtgcggg tgaacagggc cgtggttttg ccgtggtggc
     5641 gggtgaagtg cgtaatcttg ccagtcgcag cgcccaggcg gcaaaagaga tcaaagccct
     5701 cattgaagac tc
//
