LOCUS       sequence-174341-       14772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR439 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14772)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM439
FEATURES             Location/Qualifiers
     source          1..14772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2390..2406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2390..2406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2403..2425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2414..2430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2438..2444,2445..2462,2463..2468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2483..2504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2695..3371
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3438..4463
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4504..4678
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4756..4780
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5205..5999
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6086..6674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6828..6845
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6860..7000
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6926..6945)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7086..7108
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7344..7538
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7604..8677)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9106..9735)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11036..11060
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11082..11334)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11369..11386)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11393..13210,13401..13415))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          13211..13400
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(13432..13777)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     14139..14161
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14153..14170
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14154..14170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14347..14366
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14347..14365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagctttcga
      361 acaaccgcta cggttcgaca gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaattc gaacaaccgc tacggttcga cagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tggaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaatggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctggcga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaag tttgcaagca gcagattacg cccttaccgg atacctgtcc gcctttttaa
     2341 ttaatctagc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt
     2401 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2461 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2521 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2581 cggggagagg cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac
     2641 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
     2701 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
     2761 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
     2821 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     2881 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     2941 atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     3001 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     3061 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     3121 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     3181 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     3241 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     3301 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     3361 ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgagc tttcgcagat
     3421 ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     3481 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     3541 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     3601 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     3661 cggaagtgct tgacattggg gagtttagcg agagcctgac ctattgcatc tcccgccgtt
     3721 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg
     3781 tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     3841 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     3901 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     3961 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     4021 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     4081 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     4141 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     4201 agcttgcagg atcgccacga ctccgggcgt atatgctccg cattggtctt gaccaactct
     4261 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     4321 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     4381 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     4441 ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg atctgtcgat cgacaagctc
     4501 gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg
     4561 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     4621 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc
     4681 ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     4741 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     4801 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     4861 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     4921 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     4981 tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc
     5041 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     5101 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     5161 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     5221 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     5281 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     5341 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     5401 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     5461 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     5521 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     5581 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     5641 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     5701 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     5761 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     5821 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     5881 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     5941 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
     6001 acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6841 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     6901 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     6961 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     7021 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     7081 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     7141 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
     7201 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
     7261 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
     7321 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
     7381 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
     7441 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
     7501 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
     7561 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
     7621 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
     7681 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
     7741 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
     7801 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
     7861 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
     7921 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
     7981 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
     8041 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
     8101 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
     8161 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
     8221 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
     8281 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
     8341 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
     8401 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
     8461 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
     8521 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
     8581 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
     8641 cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt
     8701 tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt
     8761 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
     8821 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
     8881 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
     8941 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
     9001 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
     9061 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
     9121 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
     9181 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
     9241 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
     9301 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
     9361 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
     9421 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
     9481 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
     9541 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
     9601 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
     9661 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
     9721 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
     9781 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
     9841 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
     9901 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
     9961 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
    10021 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
    10081 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
    10141 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
    10201 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
    10261 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
    10321 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
    10381 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
    10441 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
    10501 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
    10561 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
    10621 agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc
    10681 gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct
    10741 agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg
    10801 gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg
    10861 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac
    10921 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
    10981 cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta
    11041 cccgccaata tatcctgtca aacactgata gtttaattcc cgatctagta acatagatga
    11101 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
    11161 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
    11221 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
    11281 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa
    11341 attcgagctg gtcacctgta attcacacgt ggtggtggtg gtggtggcta gcttgtttgc
    11401 ctccctgctg cggtttttca ccgaagttca tgccagtcca gcgtttttgc agcagaaaag
    11461 ccgccgactt cggtttgcgg tcgcgagtga agatcccttt cttgttaccg ccaacgcgca
    11521 atatgccttg cgaggtcgca aaatcggcga aattccatac ctgttcaccg acgacggcgc
    11581 tgacgcgatc aaagacgcgg tgatacatat ccagccatgc acactgatac tcttcactcc
    11641 acatgtcggt gtacattgag tgcagcccgg ctaacgtatc cacgccgtat tcggtgatga
    11701 taatcggctg atgcagtttc tcctgccagg ccagaagttc tttttccagt accttctctg
    11761 ccgtttccaa atcgccgctt tggacatacc atccgtaata acggttcagg cacagcacat
    11821 caaagagatc gctgatggta tcggtgtgag cgtcgcagaa cattacattg acgcaggtga
    11881 tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag tggcgcgaaa tattcccgtg
    11941 caccttgcgg acgggtatcc ggttcgttgg caatactcca catcaccacg cttgggtggt
    12001 ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg cgcttgctga gtttccccgt
    12061 tgactgcctc ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta
    12121 aagagaggtt aaagccgaca gcagcagttt catcaatcac cacgatgcca tgttcatctg
    12181 cccagtcgag catctcttca gcgtaagggt aatgcgaggt acggtaggag ttggccccaa
    12241 tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt atcgaatcct ttgccacgca
    12301 agtccgcatc ttcatgacga ccaaagccag taaagtagaa cggtttgtgg ttaatcagga
    12361 actgttcgcc cttcactgcc actgaccgga tgccgacgcg aagcgggtag atatcacact
    12421 ctgtctggct tttggctgtg acgcacagtt catagagata accttcaccc ggttgccaga
    12481 ggtgcggatt caccacttgc aaagtcccgc tagtgccttg tccagttgca accacctgtt
    12541 gatccgcatc acgcagttca acgctgacat caccattggc caccacctgc cagtcaacag
    12601 acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt
    12661 tcggcgtggt gtagagcatt acgctgcgat ggattccggc atagttaaag aaatcatgga
    12721 agtaagactg ctttttcttg ccgttttcgt cggtaatcac cattcccggc gggatagtct
    12781 gccagttcag ttcgttgttc acacaaacgg tgatacgtac acttttcccg gcaataacat
    12841 acggcgtgac atcggcttca aatggcgtat agccgccctg atgctccatc acttcctgat
    12901 tattgaccca cactttgccg taatgagtga ccgcatcgaa acgcagcacg atacgctggc
    12961 ctgcccaacc tttcggtata aagacttcgc gctgatacca gacgttgccc gcataattac
    13021 gaatatctgc atcggcgaac tgatcgttaa aactgcctgg cacagcaatt gcccggcttt
    13081 cttgtaacgc gctttcccac caacgctgat caattccaca gttttcgcga tccagactga
    13141 atgcccacag gccgtcgagt tttttgattt cacgggttgg ggtttctaca ggacggacga
    13201 gtcgtcggtt ctgtaactat catcatcatc atagacacac gaaataaagt aatcagatta
    13261 tcagttaaag ctatgtaata tttacaccat aaccaatcaa ttaaaaaata gatcagttta
    13321 aagaaagatc aaagctcaaa aaaataaaaa gagaaaaggg tcctaaccaa gaaaatgaag
    13381 gagaaaaact agaaatttac cctcagatct accatggtca agagtccccc gtgttctctc
    13441 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
    13501 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
    13561 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
    13621 ggcagaggca tcttcaacga tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc
    13681 accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat ggaatccgag
    13741 gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt cttctgagac
    13801 tgtatctttg atatttttgg agtagacaag tgtgtcgtgc tccaccatgt tgacgaagat
    13861 tttcttcttg tcattgagtc gtaagagact ctgtatgaac tgttcgccag tctttacggc
    13921 gagttctgtt aggtcctcta tttgaatctt tgactccatg aagctaaact gaaggcggga
    13981 aacgacaatc tgatccaagc tcaagctgct ctagcattcg ccattcaggc tgcgcaactg
    14041 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    14101 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    14161 gacggccagt gccaagcttg catgcctgca gttaattaag tttgcaagca gcagattacg
    14221 cgcagaaaaa aaggatctca aggagatcct ttggatcaca aaaaaaaaag cccgctcatt
    14281 aggcgggtta aattcccggg tactgcagcc gattcattaa tgcaggttaa cctggcttat
    14341 cgaaattaat acgactcact atagggagac cggcagatct gatccctact ccaaaaatgt
    14401 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatttc
    14461 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtgga
    14521 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
    14581 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtgggaaa
    14641 agaaggcgtt ccaaccacgt cttcaaagca agtggattga tgtgacatct ccactgacgt
    14701 aagggatgac gcacaatccc gcccctactc caaaaatgtc aaagatactg tctcagaaga
    14761 ccaaagggct at
//
