LOCUS       sequence-174348-       14391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disturb the expression of miR5144 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM5144
FEATURES             Location/Qualifiers
     source          1..14391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2311..2328)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2311..2327)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2320..2342)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2704..3049
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3066..3080,3271..5088)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          3081..3270
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             5095..5112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5147..5399
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5421..5445
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6746..7375
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7804..8877
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8943..9137
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9373..9395)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9481..9621
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9536..9555
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9636..9653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9807..10395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10482..11276)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11701..11725
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11803..11977
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12018..13043)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13116..13461)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    13652..13673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13688..13693,13694..13711,13712..13718)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13726..13742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13731..13753
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13750..13766
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13750..13766
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13966..13985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13966..13984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttccg accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttgtct
      361 cttcagccta agcacaagaa gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatgt ctcttcagcc taagcacaag aagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctacctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattt
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgccca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ccttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaac tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg
     2341 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     2401 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2461 cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttagctt
     2521 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
     2581 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
     2641 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
     2701 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
     2761 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
     2821 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
     2881 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
     2941 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
     3001 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct
     3061 tgaccatggt agatctgagg gtaaatttct agtttttctc cttcattttc ttggttagga
     3121 cccttttctc tttttatttt tttgagcttt gatctttctt taaactgatc tattttttaa
     3181 ttgattggtt atggtgtaaa tattacatag ctttaactga taatctgatt actttatttc
     3241 gtgtgtctat gatgatgatg atagttacag aaccgacgac tcgtccgtcc tgtagaaacc
     3301 ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac
     3361 tgtggaattg atcagcgttg gtgggaaagc gcgttacaag aaagccgggc aattgctgtg
     3421 ccaggcagtt ttaacgatca gttcgccgat gcagatattc gtaattatgc gggcaacgtc
     3481 tggtatcagc gcgaagtctt tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt
     3541 ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt gatggagcat
     3601 cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc cgggaaaagt
     3661 gtacgtatca ccgtttgtgt gaacaacgaa ctgaactggc agactatccc gccgggaatg
     3721 gtgattaccg acgaaaacgg caagaaaaag cagtcttact tccatgattt ctttaactat
     3781 gccggaatcc atcgcagcgt aatgctctac accacgccga acacctgggt ggacgatatc
     3841 accgtggtga cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg
     3901 gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt tgcaactgga
     3961 caaggcacta gcgggacttt gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt
     4021 tatctctatg aactgtgcgt cacagccaaa agccagacag agtgtgatat ctacccgctt
     4081 cgcgtcggca tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg
     4141 ttctacttta ctggctttgg tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat
     4201 aacgtgctga tggtgcacga ccacgcatta atggactgga ttggggccaa ctcctaccgt
     4261 acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg
     4321 gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg tttcgaagcg
     4381 ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg
     4441 cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg
     4501 tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca
     4561 ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgcgt caatgtaatg
     4621 ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt
     4681 tattacggat ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa
     4741 gaacttctgg cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg
     4801 gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt
     4861 gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag
     4921 gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag
     4981 aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc
     5041 tggactggca tgaacttcgg tgaaaaaccg cagcagggag gcaaacaagc tagccaccac
     5101 caccaccacc acgtgtgaat tacaggtgac cagctcgaat ttccccgatc gttcaaacat
     5161 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     5221 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     5281 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     5341 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     5401 ggaattaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     5461 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     5521 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
     5581 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
     5641 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     5701 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     5761 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     5821 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     5881 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
     5941 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
     6001 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
     6061 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
     6121 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc
     6181 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
     6241 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
     6301 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
     6361 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
     6421 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
     6481 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
     6541 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
     6601 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
     6661 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
     6721 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
     6781 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
     6841 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
     6901 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
     6961 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
     7021 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
     7081 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
     7141 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
     7201 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
     7261 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
     7321 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
     7381 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
     7441 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
     7501 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
     7561 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
     7621 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
     7681 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
     7741 aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
     7801 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     7861 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     7921 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     7981 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     8041 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     8101 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     8161 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac
     8221 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     8281 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     8341 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     8401 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     8461 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     8521 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     8581 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     8641 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     8701 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     8761 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     8821 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     8881 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
     8941 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     9001 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     9061 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     9121 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     9181 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     9241 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     9301 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
     9361 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     9421 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     9481 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     9541 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     9601 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    10261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10441 gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt
    10501 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
    10561 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
    10621 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
    10681 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
    10741 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
    10801 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
    10861 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    10921 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    10981 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    11041 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    11101 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    11161 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    11221 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    11281 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    11341 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
    11401 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
    11461 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
    11521 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
    11581 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
    11641 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
    11701 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
    11761 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
    11821 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
    11881 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
    11941 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
    12001 cccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca
    12061 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
    12121 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
    12181 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
    12241 cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag
    12301 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
    12361 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
    12421 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
    12481 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
    12541 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
    12601 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
    12661 ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc
    12721 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg aacggcggga gatgcaatag
    12781 gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat
    12841 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
    12901 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
    12961 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
    13021 gcggtgagtt caggcttttt catatctcat tgccccccgg atctgcgaaa gctcgagaga
    13081 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
    13141 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
    13201 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
    13261 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
    13321 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
    13381 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
    13441 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
    13501 ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca
    13561 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    13621 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    13681 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    13741 aatttcacac aggaaacagc tatgacatga ttacgaattc gagctcggta cccggggatc
    13801 ctctagctag attaattaag tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    13861 aggagatcct ttggatcaca aaaaaaaagc ccgctcatta ggcgggttaa attcccgggt
    13921 actgcagccg attcattaat gcaggttaac ctggcttatc gaaattaata cgactcacta
    13981 tagggagacc ggcagatctg atccctactc caaaaatgtc aaagatacag tctcagaaga
    14041 ccaaagggct attgagactt ttcaacaaag ggtaatttcg ggaaacctcc tcggattcca
    14101 ttgcccagct atctgtcact tcatcgaaag gacagtggaa aaggaaggtg gctcctacaa
    14161 atgccatcat tgcgataaag gaaaggctat cattcaagat gcctctgccg acagtggtcc
    14221 caaagatgga cccccaccca cgaggagcat cgtgggaaaa gaagacgttc caaccacgtc
    14281 ttcaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca
    14341 cccctactcc aaaaatgtca aagatactgt ctcagaagac caaagggcta t
//
