LOCUS       sequence-174366-       14099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disturb the expression of miR164 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14099)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM164
FEATURES             Location/Qualifiers
     source          1..14099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..795)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1220..1244
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1322..1496
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1537..2562)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(2629..3305)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(3373..3392)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3374..3392)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3591..3607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3591..3607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3604..3626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3615..3631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3639..3645,3646..3663,3664..3669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3684..3705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4235..4580
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        complement(5497..5515)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5498..5515)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(5586..6245)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKEITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6160..6179
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(6282..6287,6288..6304,6305..6310))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        6645..6691
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(6710..6724,6915..8732)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          6725..6914
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             8739..8756
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      8791..9043
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9065..9089
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10390..11019
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11448..12521
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12587..12781
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13017..13039)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13125..13265
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13180..13199
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13280..13297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13451..14039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
       61 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
      121 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
      181 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
      241 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
      301 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
      361 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
      421 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
      481 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
      541 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
      601 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
      661 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
      721 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
      781 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
      841 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
      901 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
      961 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     1021 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     1081 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     1141 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     1201 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     1261 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
     1321 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
     1381 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
     1441 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg
     1501 agcttgtcga tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt
     1561 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
     1621 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
     1681 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
     1741 gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc
     1801 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
     1861 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
     1921 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
     1981 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
     2041 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
     2101 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
     2161 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac
     2221 cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga
     2281 acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg
     2341 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
     2401 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
     2461 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
     2521 aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga
     2581 tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
     2641 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     2701 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     2761 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     2821 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     2881 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     2941 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     3001 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
     3061 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     3121 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaatgata gcctttcctt
     3181 tatcgcaatg atggcatttg taggagccac cttccttttc cactgtcctt tcgatgaagt
     3241 gacagatagc tgggcaatgg aatccgagga ggtttcccga aattaccctt tgttgaaaag
     3301 tctcaatagc cctttggtct tctgagactg tatctttggc atttttggag tagggatcag
     3361 atctaccggt ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt
     3421 aatgaatcgg ctgcagtacc cgggaattta acccgcctaa tgagcgggct tttttttgtg
     3481 atccaaagga tctccttgag atcctttttt tctgcgcgta atctgctgct tgcaaactta
     3541 attaatctag ctagaggatc cccgggtacc gagctcgaat tcgtaatcat gtcatagctg
     3601 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3661 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3721 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3781 gcggggagag gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca
     3841 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     3901 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     3961 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     4021 aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     4081 ccacgaggag catcgtggga aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     4141 gatgtgacat ctccactgac gtaagggatg acgcacaatc ccgcccctac tccaaaaatg
     4201 tcaaagatac tgtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaattt
     4261 cgggaaacct cctcggattc cattgcccag ctacctgtca cctcatcgaa aggacagtag
     4321 aaaaggaagg tggctcctac aaatgccatc atcgcgataa aggaaaggct atcattcaag
     4381 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     4441 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgaca
     4501 taagggatga cgcacaaccc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     4561 catttcattt ggagaggaca gcccaagctt ggtaccctca cgtgcccgta tgcttctcca
     4621 gttgttgttg ttatggtcta atttaaatat ggtctaaaga agaagaatct cacgtgcccg
     4681 tatgcttctc caggatccac tagtgaattc ggtacgctga aatcaccagt ctctctctac
     4741 aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc gataagggaa
     4801 attagggttc ttatagggtt tcgctcatgt gttgagcata taagagaccc ttagtatgta
     4861 tttgtatttg taaaatactc ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     4921 actaaaatcc agatctccta aagtccctat agatctttgt cgtgaatata aaccagacac
     4981 gagacgacta gacctggagc ccagacgccg ttcgaagcta gaagtaccgc ttaggcagga
     5041 ggccgttagg gaaaagatgc taaggcaggg ttggttacgt tgactccccc gtaggtttgg
     5101 tttaaatatg atgaagtgga cggaaggaag gaggaagaca aggaaggata aggttgcagg
     5161 ccctgtgcaa ggtaagaaga tggaaatttg atagaggtac gctactatac ttatactata
     5221 cgctaaggga atgattgtat ttatacccta taccccctaa taacccctta tcaatttaag
     5281 aaataatccg cataagcccc cgcttaaaaa ttggtatcag agccatgaat aggtctatga
     5341 ccaaaactca agaggataaa acctcaccaa aatacgaaag agtccttaac tctaaagata
     5401 aaagatcctt caagatcaaa actagttccc tcacaccggt gacggggatc gcatgcgacc
     5461 tctagaccat cacattggag cttcagctgc tcgagttcta tagtgtcacc taaatcgtat
     5521 gtgtacgata cataaggtta tgtattaatt gtagccgcgt tctaacgtcg acaggcctgt
     5581 accaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     5641 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     5701 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     5761 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     5821 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     5881 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     5941 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatattacc
     6001 agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag gcgggcaaga
     6061 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     6121 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     6181 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttctttc
     6241 tccattttag cttccttagc tcctgaaaat ctcgataact cattaaaact tatcgatgat
     6301 aagctgtcaa acatgagaat ttagcccgcc taatgagcgg gctttttgct gtgatccgcg
     6361 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6421 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cccctggaag
     6481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6541 taattaactg caggcatgca agcttccttt tgtctccctt ttgtctccct tttgtctccc
     6601 ttttgtctcc cttttgtctc ccttttgtct cccttttgtc tctcgcaaga cccttcctct
     6661 atataaggaa gttcatttca tttggagaga acacggggga ctcttgacca tggtagatct
     6721 gagggtaaat ttctagtttt tctccttcat tttcttggtt aggacccttt tctcttttta
     6781 tttttttgag ctttgatctt tctttaaact gatctatttt ttaattgatt ggttatggtg
     6841 taaatattac atagctttaa ctgataatct gattacttta tttcgtgtgt ctatgatgat
     6901 gatgatagtt acagaaccga cgactcgtcc gtcctgtaga aaccccaacc cgtgaaatca
     6961 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
     7021 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
     7081 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
     7141 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
     7201 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
     7261 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt
     7321 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
     7381 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca
     7441 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
     7501 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca
     7561 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga
     7621 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
     7681 gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt
     7741 cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct
     7801 ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc
     7861 acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
     7921 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
     7981 ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag
     8041 aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta
     8101 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
     8161 aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc
     8221 gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca
     8281 ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg
     8341 tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc
     8401 aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc
     8461 tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt
     8521 atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg
     8581 attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc
     8641 gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact
     8701 tcggtgaaaa accgcagcag ggaggcaaac aagctagcca ccaccaccac caccacgtgt
     8761 gaattacagg tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     8821 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     8881 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     8941 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     9001 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag
     9061 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     9121 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     9181 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
     9241 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     9301 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     9361 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     9421 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     9481 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     9541 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     9601 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     9661 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     9721 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     9781 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     9841 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     9901 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     9961 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
    10021 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
    10081 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
    10141 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
    10201 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
    10261 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
    10321 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
    10381 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
    10441 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
    10501 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
    10561 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
    10621 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
    10681 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
    10741 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
    10801 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
    10861 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
    10921 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
    10981 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
    11041 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
    11101 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
    11161 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
    11221 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
    11281 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
    11341 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc
    11401 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
    11461 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
    11521 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
    11581 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
    11641 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
    11701 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
    11761 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
    11821 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
    11881 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
    11941 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
    12001 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
    12061 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
    12121 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
    12181 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
    12241 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
    12301 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
    12361 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
    12421 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    12481 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    12541 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
    12601 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    12661 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    12721 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    12781 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    12841 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    12901 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    12961 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    13021 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    13081 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    13141 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    13201 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    13261 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    13321 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    13381 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    13441 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    13501 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    13561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    13621 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    13681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    13741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    13801 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    13861 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    13921 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    13981 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    14041 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaa
//
