LOCUS       sequence-174460-        7812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing UAS-myr4x::CLIPf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: UAS-myr::4xCLIPf
FEATURES             Location/Qualifiers
     source          1..7812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        232..336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(337..405,406..1197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(555..574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1368..1956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1857..1876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2043..2112
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2337..2355)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2807..2878,3280..3553,3628..4282,4344..4659,4863..4994,
                     5065..5679)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6709..6803
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6837..6931
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6962..7200
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7147..7168
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 ctctcaaagt acgcagtcag gaacgtgcgc gacatgtcgt cgggaaactc gcgcggaaac
       61 atgttgttgt aaccgaacgg gtcccatagc gccaaaacca aatctgccag cgtcaataga
      121 atgagcacga tgccgacaat ggagctggct tggatagcga ttcgagttaa cggttaatgt
      181 catgataata atggtttctt agactcaggt ggcacttttc ggggaaatgt gcgcggaacc
      241 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      301 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      361 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      421 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      481 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      541 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
      601 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      661 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      721 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      781 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
      841 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
      901 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
      961 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1021 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1081 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1141 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     1201 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1261 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1321 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1501 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1741 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1801 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1861 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1921 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1981 cggttcctgg ccttttgctg gccttttgct cacatgttac cgtcgacgat gtaggtcacg
     2041 gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
     2101 acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg
     2161 aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg
     2221 agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc
     2281 tcgacggtca cggcgggcat gtcgacaagc cgaacatatg ggcgcgccta gtatgtatgt
     2341 aagttaataa aacccatttt tgcggaaagt agataaaaaa aacatttttt ttttttactg
     2401 cactggatat cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt
     2461 gatgtaccag gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg
     2521 ctgtttgcct ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct
     2581 atctctttcg ccaccgtttg tagcgttacg tagcgtcaat gtccgccttc agttgcattt
     2641 tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc
     2701 tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga
     2761 ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga
     2821 tcaggagcta ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt
     2881 gagtagtaca tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca
     2941 tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa
     3001 acaaatagtg acacgaaaca gattattctg gtagctgttc tcgctatata agacaatttt
     3061 tgagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactata ttctttttta
     3121 caaaaaatat aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa
     3181 gtataaacct acttcgtagg atacttcggg gtactttttg ttcggggtta gatgagcata
     3241 acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg agcggcttcg
     3301 cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct ccggccaccc
     3361 agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc
     3421 tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg
     3481 gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat
     3541 ttgctcaaga acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa
     3601 gggtccaatt accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg
     3661 ccgtgatggg cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat
     3721 cgccgcaggg catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg
     3781 acgccaagga gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct
     3841 ccctaacggc cagggaacac ctgattttcc aagccatggt gcggatgcca cgacatctga
     3901 cctatcggca gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat
     3961 gtcagcacac gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga
     4021 agcgtctggc attcgcctcc gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc
     4081 ccacctccgg actggactcc tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt
     4141 cgcagaaggg caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc
     4201 tctttgacaa gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg
     4261 aagccgtcga cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagtattac
     4321 ataacatctc aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg
     4381 gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg
     4441 atcgccaaga tatgcgacaa ttttgccatt agcaaagtag cccgggatat ggagcagttg
     4501 ttggccacca aaaatctgga gaagccactg gagcagccgg agaatgggta cacctacaag
     4561 gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag
     4621 gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac
     4681 aaatgatata acgcttacaa ttcttggaaa caaattcgct agattttaga tagaattgcc
     4741 tgattccaca cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaag
     4801 ataaataaat ttcatttaac tcgcgaatat taatgagatg cgagtaacat tttaatttgc
     4861 agatggttgc catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggtg
     4921 tgatgaatat caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct
     4981 ttgccacgat aaatgtaagt catgtttaga atacatttgc atttcaataa tttactaact
     5041 ttctaatgaa tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag
     5101 gcccgaagtc gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaattg
     5161 ccgctttttc tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg
     5221 cgggccggag tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg
     5281 tcaacgtcct tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct
     5341 gtgggtccgc cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc
     5401 tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag
     5461 ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg
     5521 aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc
     5581 gatctgccgc tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca
     5641 tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac
     5701 tgcttgtttt ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa
     5761 aagctaatgt aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta
     5821 tttccccttc gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta
     5881 cacacctgca taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat
     5941 tttttatata catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca
     6001 cgtttcgtag ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg
     6061 ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc
     6121 cgagtggttc atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt
     6181 tgctccaata tatttgtata tatttccctc acagctatat ttattctaat ttaatattat
     6241 gactttttaa ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg
     6301 aaagtgacat ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa
     6361 tagtgttgtt tatatatatc aaaaataaca actataataa taagaataca tttaatttag
     6421 aaaatgcttg gatttcactg gaactagggc gcgcctccgg aacataatgg tgcagggcgc
     6481 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     6541 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     6601 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     6661 gatcatgcgc acccgtggcc agggccgcaa gcttgcatgc ctgcaggtcg gagtactgtc
     6721 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     6781 cgagcggagt actgtcctcc gagcggagac tctagcccta gggcatgcct gcaggtcgga
     6841 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     6901 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgctagc gacgtcgagc
     6961 gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa
     7021 gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac
     7081 aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa
     7141 tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagaa gagaactctg
     7201 aataattaca aattatataa caaaatgggc aacaaatgct gcagcaagcg acaggatcag
     7261 gaactggcac tggcctatcc cactgggggc tacaagaaat ccgactacac ctttggccag
     7321 acgcacatca acagcagcgg cggcggcaac atgggcggcg ttcttggcca gaagcataac
     7381 aacggtggct cgctggactc gcgctacacg cccgatccca atcatcgggg tccgttgaaa
     7441 atcggcggaa agggcggcgt tgacatcatc agaccacgcg tgatggataa ggattgcgag
     7501 atgaagcgta caacattgga ttccccgttg ggaaagttgg agttgtccgg atgcgagcag
     7561 ggattgcacc gcattatctt cttgggaaag ggcacctccg ctgctgatgc tgtcgaggtc
     7621 cccgctccgg ctgctgtctt gggaggcccc gagcccttga tccaggctac ggcttggttg
     7681 aacgcttact tccaccagcc cgaggctatt gaggagttcc ccgtccccgc tttgcaccac
     7741 cccgtcttcc agcaggagtc cttcacgcgt caggtcttgt ggaagttgct gaaggtcgtg
     7801 aagttcggcg ag
//
