LOCUS       sequence-17455-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Devroe E, Erdjument-Bromage H, Tempst P, Silver PA
  TITLE     Human Mob proteins regulate the NDR1 and NDR2 serine-threonine 
            kinases.
  JOURNAL   J Biol Chem. 2004 Jun 4. 279(23):24444-51.
  PUBMED    15067004
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Jun 4. 279(23):24444-51."
COMMENT     Sequence Label: pPS2977
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..62
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggccga ttacaaggat gacgacgata
       61 agaattcgat ggcaatgaca ggctcaacac cttgctcatc catgagtaac cacacaaagg
      121 aaagggtgac aatgaccaaa gtgacactgg agaattttta tagcaacctt atcgctcaac
      181 atgaagaacg agaaatgaga caaaagaagt tagaaaaggt gatggaagaa gaaggcctaa
      241 aagatgagga gaaacgactc cggagatcag cacatgctcg gaaggaaaca gagtttcttc
      301 gtttgaagag aacgagactt ggattggaag attttgagtc cttaaaagta ataggcagag
      361 gagcatttgg tggggtacgg cttgttcaga agaaagatac gggacatgtg tatgcaatga
      421 aaatactccg taaagcagat atgcttgaaa aagagcaggt tggccacatt cgtgcggagc
      481 gtgacattct agtggaggca gacagtttgt gggttgtgaa aatgttctat agttttcagg
      541 ataagctaaa cctctaccta atcatggagt tcctgcctgg aggggacatg atgaccttgt
      601 tgatgaaaaa agacactctg acagaagagg agactcagtt ttatatagca gaaacagtat
      661 tagccataga ctctattcac caacttggat tcatccacag agacatcaaa ccagacaacc
      721 ttcttttgga cagcaagggc catgtgaaac tttctgactt tggtctttgc acaggactga
      781 aaaaagcaca taggacagaa ttttatagga atctgaacca cagcctcccc agtgatttca
      841 ctttccagaa catgaattcc aaaaggaaag cagaaacctg gaaaaagaaa tagacgtcag
      901 ctagcccttc tccacagtag gcactcctga ctacattgct cctgaggtgt tcatgcagac
      961 cgggtacaac aagctctgtg attggt
//
