LOCUS       sequence-174578-        7645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing UAS-myr4x::SNAPf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: UAS-myr::4xSNAPf
FEATURES             Location/Qualifiers
     source          1..7645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        222..326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(327..395,396..1187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(545..564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1358..1946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1847..1866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2033..2102
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2327..2345)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2797..2868,3270..3543,3618..4272,4334..4649,4853..4984,
                     5055..5669)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6699..6793
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6827..6921
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6952..7190
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7137..7158
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 acgcagtcag gaacgtgcgc gacatgtcgt cgggaaactc gcgcggaaac atgttgttgt
       61 aaccgaacgg gtcccatagc gccaaaacca aatctgccag cgtcaataga atgagcacga
      121 tgccgacaat ggagctggct tggatagcga ttcgagttaa cggttaatgt catgataata
      181 atggtttctt agactcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      541 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      841 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
      901 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      961 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1021 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1081 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1141 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1201 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1501 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1981 ccttttgctg gccttttgct cacatgttac cgtcgacgat gtaggtcacg gtctcgaagc
     2041 cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca
     2101 tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc
     2161 gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac
     2221 gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca
     2281 cggcgggcat gtcgacaagc cgaacatatg ggcgcgccta gtatgtatgt aagttaataa
     2341 aacccatttt tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat
     2401 cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag
     2461 gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct
     2521 ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg
     2581 ccaccgtttg tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt
     2641 ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa
     2701 ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg
     2761 gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta
     2821 ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca
     2881 tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc
     2941 tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg
     3001 acacgaaaca gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata
     3061 tcatgatcaa gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat
     3121 aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct
     3181 acttcgtagg atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag
     3241 ttgatatttg agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca
     3301 ttaaccaggg cttcgggcag gccaaaaact acggcacgct ccggccaccc agtccgccgg
     3361 aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata
     3421 tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga
     3481 cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga
     3541 acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt
     3601 accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg
     3661 cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg
     3721 catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga
     3781 gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc
     3841 cagggaacac ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca
     3901 gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac
     3961 gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc
     4021 attcgcctcc gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg
     4081 actggactcc tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg
     4141 caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa
     4201 gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga
     4261 cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc
     4321 aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg
     4381 tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga
     4441 tatgcgacaa ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca
     4501 aaaatctgga gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt
     4561 tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc
     4621 tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata
     4681 acgcttacaa ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca
     4741 cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat
     4801 ttcatttaac tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc
     4861 catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat
     4921 caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat
     4981 aaatgtaagt catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa
     5041 tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc
     5101 gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc
     5161 tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag
     5221 tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct
     5281 tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc
     5341 cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag
     5401 tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga
     5461 ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt
     5521 gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc
     5581 tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc
     5641 taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt
     5701 ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt
     5761 aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc
     5821 gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca
     5881 taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata
     5941 catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag
     6001 ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc
     6061 ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc
     6121 atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata
     6181 tatttgtata tatttccctc acagctatat ttattctaat ttaatattat gactttttaa
     6241 ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat
     6301 ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt
     6361 tatatatatc aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg
     6421 gatttcactg gaactagggc gcgcctccgg aacataatgg tgcagggcgc tgacttccgc
     6481 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca
     6541 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa
     6601 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc
     6661 acccgtggcc agggccgcaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg
     6721 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     6781 actgtcctcc gagcggagac tctagcccta gggcatgcct gcaggtcgga gtactgtcct
     6841 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     6901 agcggagtac tgtcctccga gcggagactc tagcgctagc gacgtcgagc gccggagtat
     6961 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     7021 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt
     7081 aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa
     7141 ctactgaaat ctgccaagaa gtaattattg aatacaagaa gagaactctg aataattaca
     7201 aattatataa caaaatgggc aacaaatgct gcagcaagcg acaggatcag gaactggcac
     7261 tggcctatcc cactgggggc tacaagaaat ccgactacac ctttggccag acgcacatca
     7321 acagcagcgg cggcggcaac atgggcggcg ttcttggcca gaagcataac aacggtggct
     7381 cgctggactc gcgctacacg cccgatccca atcatcgggg tccgttgaaa atcggcggaa
     7441 agggcggcgt tgacatcatc agaccacgcg tgatggacaa agactgcgaa atgaagcgca
     7501 ccaccctgga tagccctctg ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc
     7561 gtatcatctt cctgggcaaa ggaacatctg ccgccgacgc cgtggaagtg cctgccccag
     7621 ccgccgtgct gggcggacca gagcc
//
