LOCUS       sequence-174641-        4608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Eid A, Rodriguez-Terrones D, Burton A, Torres-Padilla ME
  TITLE     SUV4-20 activity in the preimplantation mouse embryo controls timely
            replication.
  JOURNAL   Genes Dev. 2016 Nov 15;30(22):2513-2526. Epub 2016 Dec 5.
  PUBMED    27920088
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2016-11-15"; volume: "30(22)"; pages: "2513-2526. Epub
            2016 Dec 5"
COMMENT     Sequence Label: pRN3P-HA-Suv4-20h2
FEATURES             Location/Qualifiers
     source          1..4608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           45..87
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(135..151)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(135..151)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     3'UTR           1584..1709
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(1797..1816)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1798..1816)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1823..1840)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1823..1839)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1832..1854)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1981..2436)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2118..2139)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2330..2349
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2463..2567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2568..2636,2637..3428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2786..2805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3599..4187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4088..4107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4341..4358
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4475..4496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4511..4516,4517..4534,4535..4541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4549..4565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4554..4576
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4573..4589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4573..4589
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaattaaccc tcactaaagg gaacaaaagc tggagctaag cttgcttgtt ctttttgcag
       61 aagctcagaa taaacgctca actttggcag atctgccgcc atgggctacc catacgatgt
      121 tcctgactat gcgggatcca ctagttctag aatggggcct gatcgagtga cagcccgaga
      181 actgtgcgag aatgatgacc tggccaccag ccttgttctt gacccctacc tcggcttccg
      241 cacccataag atgaatgtca gtcctgtgcc caccttgcga cgacaacacc accttcgttc
      301 agcactggaa gctttcctaa ggcaacggga cctggaggct gcattccggg ccctgacgct
      361 aggaggctgg atggcccact acttccagag ccgtgcccct cggcaggagg ctgctctcaa
      421 gacccacatt ttctgctacc tccgcgcctt cctgcctgag agtggcttca ccattctgcc
      481 ctgcacccgc tactccatgg agaccaatgg ggccaagatt gtgtctaccc gtgcttggaa
      541 gaagaatgag aagctggagc tgctggtggg ctgcattgca gagctgcgtg aagaggatga
      601 agacctgctt agggccggtg agaacgactt cagcatcatg tactccaccc gaaaaaggag
      661 tgcccagctg tggcttggcc cagctgcctt catcaaccat gactgcaaac ccaactgcaa
      721 gtttgtgccc tcagatggga atactgcctg tgtgaaggtg ctacgggaca ttgagccagg
      781 ggatgaagtg acttgcttct atggtgaggg cttcttcggc gagaagaatg agcactgtga
      841 atgctatacc tgtgagagga aaggcgaggg agctttcaga cttcagccca gggagcctga
      901 gctgcgacca aagcccctag acaagtatga gctccgagag acaaagaggc gtctgcagca
      961 gggcttggtc agcagccagc agagcctgat gagccggtgg gcctgctccc acctgtcccc
     1021 actgcgccct gacccattct gtgctgcctg ccagccctca tgcctgctgc ctgccagccc
     1081 ccacatggat tacttgcccc tttggctcca gcgggcgcct caaccccagc ccatagtacc
     1141 tcccaggaag cgccaccgcc gccgccgccc caggatccgc caggcttcat tgcctcctgt
     1201 cctccgcact gcctgtgtcc ccctgcaccg atggggaggc tgtggtcccc actgccagct
     1261 acgtgctgag gccatggtga ccctacacct gcgcccccag actcgctgga ccccacaaca
     1321 ggactggtac tgggcccggc gctatgggct accttctgtg gggcgtgtgg aacttacccg
     1381 cctagcccca gccctgccag ctgctcctgc ccctgctggg aacccaggtc ctgtccccac
     1441 tcctgatttc atccctaagc aggcccttgc ctttgctccg ttctgcccac ctaagcgcct
     1501 ccggctagtg gtcagtcatg gttctattga tctggacatc aatagtggtg agccataagc
     1561 ggccgcggat ctggttacca ctaaaccagc ctcaagaaca cccgaatgga gtctctaagc
     1621 tacataatac caacttacac tttacaaaat gttgtccccc aaaatgtagc cattcgtatc
     1681 tgctcctaat aaaaaraaag tttcttcaca ttctaaaaaa aaaaaaaaaa aaaaaaaaaa
     1741 aaaaaaamcc cccccccccc ccccctgcag ggccaagtcg gccggtaccc aattcgccct
     1801 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1861 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1921 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     1981 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     2041 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     2101 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     2161 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     2221 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     2281 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     2341 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     2401 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     2461 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2521 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2581 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2641 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2701 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2761 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2821 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2881 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2941 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3001 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3061 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3121 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3181 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3241 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3301 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3361 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3421 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3481 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3541 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3601 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3661 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3721 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3781 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3841 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3901 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3961 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4141 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4201 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4261 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4321 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4381 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4441 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     4501 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     4561 aacaatttca cacaggaaac agctatgacc atgattacgc caagctcg
//
