LOCUS       sequence-17469-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Kim JB, Sarraf P, Wright M, Yao KM, Mueller E, Solanes G, Lowell BB,
            Spiegelman BM
  TITLE     Nutritional and insulin regulation of fatty acid synthetase and 
            leptin gene expression through ADD1/SREBP1.
  JOURNAL   J Clin Invest. 1998 Jan 1. 101(1):1-9.
  PUBMED    9421459
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 1998 Jan 1. 101(1):1-9."
COMMENT     Sequence Label: FAS promoter luciferase
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    321..455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(345..364)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     399..418
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(636..655)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(637..655)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(662..679)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(662..678)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(671..693)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttcatttta ccaacagtac cggaatgcca
       61 agcttacggg acgctgctgc cgtctctctg gctccctcta ggccgcgccg gcgctattta
      121 aaccgcggcc atccccgcgc ggccacgcca catgggctga cagcttggct gcgccgccca
      181 ggccaatgag cgtcggggct gcgccccggg acccggcgcg ccgccgtcgg tggggtgatg
      241 cccgcgcgcg ccgcccccag ccgggaccct gcacaggcgc gcgctggagc acaaggaacg
      301 cgcagccccc ggtacccggg gatccagaca tgataagata cattgatgag tttggacaaa
      361 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      421 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
      481 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
      541 gtggtatggc tgattatgat cctctagagt cgcagatcct ctagagtcgc agatctgcaa
      601 gctctagagg agcatgcgac gtcgggccca attcgcccta tagtgagtcg tattacaatt
      661 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      721 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      781 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgca
//
