LOCUS       sequence-17482-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Yang HS, Hinds PW
  TITLE     Increased ezrin expression and activation by CDK5 coincident with 
            acquisition of the senescent phenotype.
  JOURNAL   Mol Cell. 2003 May . 11(5):1163-76.
  PUBMED    12769842
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2003 May . 11(5):1163-76."
COMMENT     Sequence Label: pBS/U6 ezrin siRNA
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..45
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(119..432)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(180..200)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(429..445)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(462..481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(463..481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(491..508)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(491..507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(500..522)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(736..755)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctggagctc caccgcggtg gcggccgctc tagaactagt ggatcccccg ggctgcagga
       61 attcaaaaag ggtcctacgc tgtgcaggcc aagcttggcc tgcacagcgt aggacccaaa
      121 caaggctttt ctccaaggga tatttatagt ctcaaaacac acaattactt tacagttagg
      181 gtgagtttcc ttttgtgctg ttttttaaaa taataattta gtatttgtat ctcttataga
      241 aatccaagcc tatcatgtaa aatgtagcta gtattaaaaa gaacagatta tctgtctttt
      301 atcgcacatt aagcctctat agttactagg aaatattata tgcaaattaa ccggggcagg
      361 ggagtagccg agcttctccc acaagtctgt gcgagggggc cggcgcgggc ctagagatgg
      421 cggcgtcgga tccactagtt ctagagcggg tacccaattc gccctatagt gagtcgtatt
      481 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      541 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      601 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
      661 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      721 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      781 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      841 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
      901 agacgggttt ttcg
//
