LOCUS       sequence-175063-       10439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 10439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN303
FEATURES             Location/Qualifiers
     source          1..10439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2622..2646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3693..3713)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3721..3738)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3721..3737)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3730..3752)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4065..4087
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4125..4143)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4211..4315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4316..4384,4385..5176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4534..4553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5347..5935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5836..5855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6062..6084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6081..6097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6081..6097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    6117..6137
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7598..7622
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7771..7782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tccttgaggg cgatcatctc cttggagatt gggtcgtagg cgtagtaacg ggagacgagg
       61 gcgaagtagt ggtctgggat ggcggtgatt tggtgggtgt aggtggtacg ggcgacggcg
      121 gaggcacgct tgtcggacca gtttccgacg acgttggtga gctcggtgag tccctgaaaa
      181 tttaaatcag attagttagt tagtaaaatc atgtttaaac ttaccttcat ggagaggaag
      241 gaggtcatga ggtgacgtcc gatgtgggac tttggtccgt tcttgatggc gaagatggag
      301 tatggggcgt tcttcttgag ggccttgttg taggaacgga cgaggttgtc cttgaggagt
      361 tggtactctt gcttgttgga ggaggagttt ccggtacggt tgacacgctt gaggactggc
      421 tcggagttac ggaggaactc gtcgaggtag acgagtgggt cgatacgtcc acgggcggag
      481 aagaagtaga tgtgacggga gacggaggtc ttggtctcgg tgacgaggca ttggatgatg
      541 actccgaggt acttgttttg gacgagcttg aaggactttg ggtcgacgtt cttgatgtcg
      601 gagaaacgtc cgcagttgat gaaggtggcg aggaagagga attggtagag ggtcttggtc
      661 ttggtgaaac gggaggtgta ctcgaaggag ttgaggatct tctcggtgat ctcccagatg
      721 gactctccct cggagaggag ggccttgagc atcttcttgg agtgggagtt tcccttgtcg
      781 gcctcctcgg aggactcgaa ttggagttgg agggaggaga cgatgtcggt gatgtcggat
      841 tggtgctttt gtccgtagta tgggatgatg gtgaactccc aggctgggat gagcttctga
      901 aaatttaaat aatcagggtt agttagtata tatatgttta aacttacctt gagggaggcc
      961 tcgaggatgg tggccttttg ggtcttgtac ttgaattgga gggacttgtt gacgatgtcg
     1021 aaggagaggg agttggagat gatggtgttg taggacatga aggtggcacg cttgatggcg
     1081 gttccgttgt gggtgatcat ccagcagagg taggtgagct cggcggcgca gagggcgatc
     1141 ttctctccgg atggacgctc gaaacgctcg acgaattgac ggacgaggac ttttggtggt
     1201 gtcttacaga gaatgtcgaa ttgtggcatt tttggtacct gtaatgaaat aaatgtgacg
     1261 ctgtcgtctc acaggaaaga cagattgtgg aaagatggag caaatttcgt ggaaaagagc
     1321 tctgaatgcg tcgtcgtcgt cttcgctctt caaatagcgt gtgtgtgtga gcacacataa
     1381 agtgtgtctg tgtgttggca caatgatttg aagagcacgg gggattatta gaccctactc
     1441 tccaaaccga aaggctcttc ttttttctgg ttccactgat ggattggtag aataaatgtg
     1501 aatagggggg aaccagcgac tatagtgagt ttattggcaa aaaaaacgag agagggaccg
     1561 gaaatttggg aagtattctt tctttttttc tgaatttggt ctaaagagac agctaatatc
     1621 gtcctcgtca attaaatttt acgagattca ttttatttct ccaaagagct aaaaatgttt
     1681 tgtaacaatc aaggttcaga cccaggggtg ggcggcaaac gattttttcc ggcaaatcgg
     1741 gaaatcggga aatcggcaat ttgcctgaat taaaatttcc ggcaaatcgg cacactgccg
     1801 gaattgaaaa tttcctgcaa ttggaaaacc ggcaaattgc ctcagtatgt ccatcatttg
     1861 tgccaggggt gttgtcggtt tgccgatttg acggaacttt tcaattcagg cagtttgccg
     1921 gtttgccgat ttaccggaaa ctgtaatttt cggcaagttt ccgatttgcc atttgccgaa
     1981 catcaatttg ccggaagtgt ttagggggat ttttttatta gacggaaaca cttaaaactg
     2041 tgcctttttg aaaatttttt ccatttttct ttagatattt tcatagaatt tgcttacttt
     2101 tcaaaataga tgtaggaaaa ttcataggat gtgcacaatt tgccgattga aattctgaaa
     2161 tttccaaaaa gtgcaaaacc acaatttgcc gaatatgttt ggcaattgcc gtcttcccgg
     2221 caaattcggc aaatcagcaa tttgcggttt accagtttgc cggaaatttt caattccggc
     2281 tatttaccga tttgccgggt atttcaatat tggcaatttg ccgatttgcc gatttgccgg
     2341 aaaaatcgtt tgccgctcac ccctgatttg tgccccggtt agaccgtcag cagttcggcg
     2401 cttgccaccg actttcgact gttccgggat cccaacagct ttttttccaa gaaagttgtt
     2461 ttgtcaactt tttgacgtag aagtttagtt attttcagtc ttgttttaat agatacgatt
     2521 cacaggacaa attggcaggc ggccgcatgc tacagccggc tcctcgagac gtacggtgcg
     2581 cgcgatgcat tcgaagatct gcccactagt gagtcgtatt aacccagctt tcttgtacaa
     2641 agtggataga atcaagcatg ctccgcgttt gctgtactat ttctcgcaga gcagtttttt
     2701 ttctcacact ttattattct gtactgcccc tccacgctat aattttttgc atagagggcc
     2761 ataaaggaaa acggtacggt aaaaataatg ggtccttttt accgttgtgt agattgtttc
     2821 taacgggatg caaattttca ctaaaactct ggaaaactga tggaattcta cgggcagccg
     2881 gcaattttag atatttgaag aatccttctt gatcagtgcc aagctttccc atggaccaaa
     2941 gatgtggatc caagatcaaa tgcacaggat ctgaactcct ttgttccaaa aacaaggatt
     3001 tcttttctat agtttgacct aaatattata gtagaacata tacatttgat gatttcatga
     3061 ataatttatt gttaaaaaat aatacatgtg ttaaaaaata attctaaaaa attaattgga
     3121 gcgtccttgg cggatatggc ggattggaag gtcataagta actcgaacta acctgtaaat
     3181 ataattaatt tagaagtaat cattttgaaa tgtttctaaa tttttgtaaa cgtaatcaat
     3241 gatttactta caaaattttt cgaaatatta gtagaaatat aaaaaaccag taaaattcaa
     3301 aagaggccag ccacacatgc atcttacaat acgaatgatg cgttttttgt cgcctcggag
     3361 aaggaatcaa gcaggctccg cgttttctgt actatttctc ccagagccgt ttattttgca
     3421 tagggggtgg gtcataaacg gagacgtttt ggtataaaat ttgccccttc ttccaccgtt
     3481 gaaaaaattg gaaaatgaac cgggacagga aaggggattt caggataatg gttcaattat
     3541 cgatgctact gtcatatctg agtagtggat ttgagcaagg tttccaaatt ttgagtacaa
     3601 gcgtctagtg catgtgtcaa aaaagtcggg caaaaattag ttttaatcaa taaggtttaa
     3661 agatcagaaa tttttgtctg acctaatggg cccaacttta ttatacatag ttgataattc
     3721 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3781 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3841 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     3901 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtgcaa tctgctctga
     3961 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4021 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4081 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     4141 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     4201 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4261 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4321 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4381 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4441 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4501 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4561 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4621 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4681 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4741 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4801 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4861 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4921 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4981 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5041 tatcattgca gcactggggt cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5101 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5161 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5221 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5281 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5341 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5401 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5461 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5521 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5581 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5641 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5701 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5761 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5821 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5881 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5941 cagcaacgcg gcctttttac ggttcctggt cttttgctgg ccttttgctc acatgttctt
     6001 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6061 cagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctatcaac
     6121 tttgtataga aaagttggag ggacttccag ctgtctgcga gccagtccaa aagggagagc
     6181 caggatccac cggagagcca ggaccagtcg gaccaccagg accatccgga caaccaggaa
     6241 acgacggaac cccaggtaat catttcatag tttttcaatc catatctaat caccacatct
     6301 ttcaggacaa cctggaccaa agggaccacc aggaccagac ggcaagccag gagccgacgg
     6361 aaacccagga cagccaggac cagtcggacc accaggaacc ccaggagagc gtggaatctg
     6421 tccaaagtac tgcgccatcg atggaggaat cttcttcgag gacggaactc gccgctaagc
     6481 ggatcgcctt taatttattc tttgccttat gcgttgctca aaataaaaca ttatttcatc
     6541 caaaataatt ttttgaggtt ttagatgaat ttgatagttt agcttgtcac tttctcctgt
     6601 gcgaagaaac ttgctaagca ggtaagcagc atccttatca agtttcgacc ttatctctca
     6661 gtagtcgcat acgtcgtatc cctcatctgc tttgttttag ccacttcaag ggaattcaga
     6721 tcactttttc tcatgtgttt tcaaatttac ttttgtggag tgaccttaat tgaccctctc
     6781 gtcataagtg gacggtgggt agttacttaa caaatttcga gtacaggata gtgtcaatgg
     6841 catgagacat gtagtagcca agcaattatg gcgaataagt ttcaaaaacc aacattctgt
     6901 caaatacaaa gacctgcaac attagagaat tcattaaata catcttcgag gaaatcaaat
     6961 tttcccgtag acgatcccgt agacctactt gtacaatatg ttctagatat acactccgaa
     7021 cttaaaatat ctcgaacatt tgtgttgaat atttatttta attctaggtt tttcaaattc
     7081 cgcacgattt ttgccatact actgcccaat agtttacatt tattgggcct ggatctgtaa
     7141 ggcacttttg ggagctccct ttcttttcct tcaaattacc catacagatc tataaatcac
     7201 aatttaatac aagattttat tcaagttaaa aacataaaag gggctttgcg gtggtcccca
     7261 tttcaccaga gaactataaa aaatataaat ttaacatcaa atgttgcaat tgattccctc
     7321 tcaaagctgc ttgaagaacc ctgattctgt caagcctatg aagatttaaa aaaaattggg
     7381 aagaccctta gttccaaaca agtgtcgttg acccagtagg cgatattctg aaaagtcata
     7441 aaatggggtt gctaaaaaat tggtcgctaa cttacattta gctaggaatg ttaattggaa
     7501 taactcataa tttacagtaa aattatcaaa aaatagcaaa aatcaataga ggaccgctac
     7561 acaggcatcc aacagtacga gttctgcgtt tttgaattca agtttgtaca aaaaagcagg
     7621 ctctttgagc caatttatcc aagtccttgt aaaaagtatt cgaaaattgt taacggataa
     7681 aatgtttatt ataatatcaa aaacaatttg tcagttgacc actttttgat atatttgaca
     7741 gaaaccggga tgaattggct caaagttagg gcgcccttct attacagggt ttctgataac
     7801 aaaccggtta ttaactccca acaagggatg atttcaattc atcatgctca aattgaccca
     7861 aattaagtta catgacaaat tcatcgccct tttcaactct tcttggctca tcatctgtta
     7921 ttctgttcct attctgtctg caccctatac ccctttgcat actctctcgt cattcctttc
     7981 ttggatagtg tcttcttctc cccagctctg ctacttctat gacttgcgcc cgctgctttt
     8041 ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct tttctccttc ctttttccct
     8101 tctcaatctt cctctttttc ccatttctgt caatcattcg aagaagaaga agaagaagac
     8161 cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc
     8221 tattccacgt cttcttcttt ttcttcttcg attcgaatca atcactccac caaaacgcat
     8281 tcgtttttgg gattcacccc gcggttcgca ataggtttct ttttcaaatt attagcgtta
     8341 taaatagaaa aatggtggag tttcaataaa aatgataatt tacaaagtgt attttgatta
     8401 catgtactca aaaaggttga aatttttcat accagttttc cggaaatcca tctgatatca
     8461 ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa caaaatatag ctggttattt
     8521 ggcaccctct aattaccatt ttcctgtcac accacactct tttccttctt ccacttcttt
     8581 ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg agcaacaacg atcgattcca
     8641 cccggctcgg cgaccttccc gtcgcaggtg agactcagaa aactagagaa accgctcaac
     8701 taactcttga tatccgattt cattcttttc tcttttcttt ttgttgaact tttccatatt
     8761 tccagatgcc acggccacca ccaagcaccg aacaaggaat cacaacggaa tcggagcttg
     8821 cgaagaaagc tcaaatcact ccgaacgacg ttttagcact tccgggaatc actcaaggta
     8881 tcttgctccc tttggatttc agtttaaaac ataaatttag gattcttatg ctccccatct
     8941 gcgaacatct ataacatcga gttcaccaag ttccaaatcc gtgatctgga cactgagcaa
     9001 gtgcttttcg agatcgccaa accggagaac gatcaggaga atgatgagtc gccacaggag
     9061 tcggcaagat acgtgcgtta tagatttgct ccaaactttt tgaaactcaa aacggtcgga
     9121 gcaactgtgg aattcaaagt aggagacatc ccaatccatc atttccgaat gatcgaacgt
     9181 cacttcttca aagatcgcct tctgaagtgt tttgactttg aattcggatt ctgtattccg
     9241 aattcacgaa acaactgtga acatatctat gagttccctc aactctctca acaactcagt
     9301 gagtcattat tctaaaaagt acaattcaaa agactaatct ctttttcagt ggacgacatg
     9361 atcaacaatc caaacgagac tcgttctgac agcttctatt tcgtcgataa caaactcgtc
     9421 atgcacaaca aagccgacta ctcatatgat gcataaatat ttaatacaaa aatgttctgg
     9481 ataattattc tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca ataattcctg
     9541 tctagtttgt tccttcccct tccccttctc atgttcaatg cattcctaag cttttcagtt
     9601 ccccccttgt ttctatattt tttcgctgtc ctgtcacact cgctaaaaac actaatcaca
     9661 cggaaatctg ttttcaataa aaactccaac tttaactcat tttcaatttc aactgaaaga
     9721 ttttttcatt agagaatgtc tagaactagg cccggggaaa gccgggttac cataagaaaa
     9781 aaaattacat tttttctcga atagtagaaa catattgcaa gactattttt cattatttca
     9841 gaacacacac tggagagaga ccgtatccat gtgctcaatg tgagaaggca ttcaccgaca
     9901 gctcaactct tacaaaacat ctgagaaccc atactggaca taaaccttat gtttgctcaa
     9961 tttgtatgat gaaattcaca cagagtggga acttgcatag gcacatgaaa actcataaat
    10021 agtttttcct attatggaag taatgtttct aaatgaatat tgccattttc tgcaaaatat
    10081 tttacaatcc acaagtctcg aacaaattta atcaatgtct tgttttgata caatttaaca
    10141 aattcactgt ggagaggatt tactataaaa acgttttaca ccagcaaaaa atcaatagaa
    10201 catttacatg ataaggagaa tatataaaaa gggggcatta taatttactg atacaatatg
    10261 gacaacattt gaaggatatg cagatacata cgcgttagat acgacggttg atgtaggagg
    10321 agaggtagtc gaggacctct tgggagatga ttccgttcca ggctgggtaa cggatggatc
    10381 cctcggcgga tcccttgagt tgctcgatgt gttgccactc ctcgattggg ttggtctcg
//
