LOCUS       sequence-175222-        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express FNTA with a FLAG tag at C terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pcDNA5/FRT/TO-FNTA-FLAG
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1117..1140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttaccgcc
      181 atggcggcca ccgagggggt cggggaggct gcgcaagggg gcgagcccgg gcagccggcg
      241 caacccccgc cccagccgca cccaccgccg ccccagcagc agcacaagga agagatggcg
      301 gccgaggctg gggaagccgt ggcgtccccc atggacgacg ggtttgtgag cctggactcg
      361 ccctcctatg tcctgtacag gcatttccgg agagttcttt tgaagtcact tcagaaggat
      421 ctacatgagg aaatgaacta catcactgca ataattgagg agcagcccaa aaactatcaa
      481 gtttggcatc ataggcgagt attagtggaa tggctaagag atccatctca ggagcttgaa
      541 tttattgctg atattcttaa tcaggatgca aagaattatc atgcctggca gcatcgacaa
      601 tgggttattc aggaatttaa actttgggat aatgagctgc agtatgtgga ccaacttctg
      661 aaagaggatg tgagaaataa ctctgtctgg aaccaaagat acttcgttat ttctaacacc
      721 actggctaca atgatcgtgc tgtattggag agagaagtcc aatacactct ggaaatgatt
      781 aaactagtac cacataatga aagtgcatgg aactatttga aagggatttt gcaggatcgt
      841 ggtctttcca aatatcctaa tctgttaaat caattacttg atttacaacc aagtcatagt
      901 tccccctacc taattgcctt tcttgtggat atctatgaag acatgctaga aaatcagtgt
      961 gacaataagg aagacattct taataaagca ttagagttat gtgaaatcct agctaaagaa
     1021 aaggacacta taagaaagga atattggaga tacattggaa gatcccttca aagcaaacac
     1081 agcacagaaa atgactcacc aacaaatgta cagcaagatt acaaggatga tgatgataag
     1141 taggcggccg ctcgagtcta gagggcccgt
//
