LOCUS       sequence-175233-        7516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of FLAG-tagged human androgen receptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Bai S, He B, Wilson EM
  TITLE     Melanoma antigen gene protein MAGE-11 regulates androgen receptor 
            function by modulating the interdomain interaction.
  JOURNAL   Mol Cell Biol. 2005 Feb;25(4):1238-57.
  PUBMED    15684378
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-02"; volume: "25(4)"; pages: "1238-57"
COMMENT     Sequence Label: pCMV-FLAG-hAR
FEATURES             Location/Qualifiers
     source          1..7516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1513..2135
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1712..1731)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2159..2179)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2164..2493
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2344..2479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2531..2550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2532..2550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2564..2580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2564..2580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2577..2599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2588..2604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2612..2618,2619..2636,2637..2642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2657..2678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2795..2812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2966..3554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3046..3065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3725..4516,4517..4585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4348..4367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4586..4690)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4717..5172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4804..4823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5014..5035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5298..5320
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5312..5329
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5313..5329
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5490..5869
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5870..6073
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6024..6044
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6120..6143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggcgaggcgg gagctgtagc cccctacggc tacactcggc cccctcaggg gctggcgggc
       61 caggaaagcg acttcaccgc acctgatgtg tggtaccctg gcggcatggt gagcagagtg
      121 ccctatccca gtcccacttg tgtcaaaagc gaaatgggcc cctggatgga tagctactcc
      181 ggaccttacg gggacatgcg tttggagact gccagggacc atgttttgcc cattgactat
      241 tactttccac cccagaagac ctgcctgatc tgtggagatg aagcttctgg gtgtcactat
      301 ggagctctca catgtggaag ctgcaaggtc ttcttcaaaa gagccgctga agggaaacag
      361 aagtacctgt gcgccagcag aaatgattgc actattgata aattccgaag gaaaaattgt
      421 ccatcttgtc gtcttcggaa atgttatgaa gcagggatga ctctgggagc ccggaagctg
      481 aagaaacttg gtaatctgaa actacaggag gaaggagagg cttccagcac caccagcccc
      541 actgaggaga caacccagaa gctgacagtg tcacacattg aaggctatga atgtcagccc
      601 atctttctga atgtcctgga agccattgag ccaggtgtag tgtgtgctgg acacgacaac
      661 aaccagcccg actcctttgc agccttgctc tctagcctca atgaactggg agagagacag
      721 cttgtacacg tggtcaagtg ggccaaggcc ttgcctggct tccgcaactt acacgtggac
      781 gaccagatgg ctgtcattca gtactcctgg atggggctca tggtgtttgc catgggctgg
      841 cgatccttca ccaatgtcaa ctccaggatg ctctacttcg cccctgatct ggttttcaat
      901 gagtaccgca tgcacaagtc ccggatgtac agccagtgtg tccgaatgag gcacctctct
      961 caagagtttg gatggctcca aatcaccccc caggaattcc tgtgcatgaa agcactgcta
     1021 ctcttcagca ttattccagt ggatgggctg aaaaatcaaa aattctttga tgaacttcga
     1081 atgaactaca tcaaggaact cgatcgtatc attgcatgca aaagaaaaaa tcccacatcc
     1141 tgctcaagac gcttctacca gctcaccaag ctcctggact ccgtgcagcc tattgcgaga
     1201 gagctgcatc agttcacttt tgacctgcta atcaagtcac acatggtgag cgtggacttt
     1261 ccggaaatga tggcagagat catctctgtg caagtgccca agatcctttc tgggaaagtc
     1321 aagcccatct atttccacac ccagtgaagc attggaaacc ctatttcccc accccagctc
     1381 atgccccctt tcagatgtct tctgcctgtt ataactctgc actactcctc tgcagtgcct
     1441 tggggaattt cctctattga tgtacagtct gtcatgagct tgcatgcctg caggtcgact
     1501 ctagaggatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     1561 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     1621 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     1681 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     1741 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     1801 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     1861 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     1921 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     1981 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg
     2041 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca
     2101 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg
     2161 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2221 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     2281 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     2341 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     2401 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     2461 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag
     2521 aaagttaatt ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg
     2581 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2641 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2701 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2761 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2821 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2881 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2941 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3001 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3061 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3121 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3181 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3241 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3301 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3361 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3421 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3481 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3541 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3601 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3721 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3781 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3841 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3901 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3961 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4021 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4081 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4141 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4201 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4261 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4321 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4381 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4441 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4501 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4561 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4621 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4681 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     4741 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4801 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4861 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4921 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4981 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5041 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5101 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5161 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     5221 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     5281 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgat
     5341 ctatacattg aatcaatatt ggcaattagc catattagtc attggttata tagcataaat
     5401 caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat
     5461 tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta
     5521 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     5581 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     5641 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     5701 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat
     5761 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga
     5821 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     5881 ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     5941 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     6001 tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     6061 tataagcaga gctcgtttag tgaaccgtca gaattcagat ctagccaagc tcaaggatgg
     6121 actacaaaga cgatgacgat aaagaagtgc agttagggct gggaagggtc taccctcggc
     6181 cgccgtccaa gacctaccga ggagctttcc agaatctgtt ccagagcgtg cgcgaagtga
     6241 tccagaaccc gggccccagg cacccagagg ccgcgagcgc agcacctccc ggcgccagtt
     6301 tgctgctgct gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     6361 agcagcagca gcagcagcag cagcaagaga ctagccccag gcagcagcag cagcagcagg
     6421 gtgaggatgg ttctccccaa gcccatcgta gaggccccac aggctacctg gtcctggatg
     6481 aggaacagca accttcacag ccgcagtcgg ccctggagtg ccaccccgag agaggttgcg
     6541 tcccagagcc tggagccgcc gtggccgcca gcaaggggct gccgcagcag ctgccagcac
     6601 ctccggacga ggatgactca gctgccccat ccacgttgtc cctgctgggc cccactttcc
     6661 ccggcttaag cagctgctcc gctgacctta aagacatcct gagcgaggcc agcaccatgc
     6721 aactccttca gcaacagcag caggaagcag tatccgaagg cagcagcagc gggagagcga
     6781 gggaggcctc gggggctccc acttcctcca aggacaatta cttagggggc acttcgacca
     6841 tttctgacaa cgccaaggag ttgtgtaagg cagtgtcggt gtccatgggc ctgggtgtgg
     6901 aggcgttgga gcatctgagt ccaggggaac agcttcgggg ggattgcatg tacgccccac
     6961 ttttgggagt tccacccgct gtgcgtccca ctccttgtgc cccattggcc gaatgcaaag
     7021 gttctctgct agacgacagc gcaggcaaga gcactgaaga tactgctgag tattcccctt
     7081 tcaagggagg ttacaccaaa gggctagaag gcgagagcct aggctgctct ggcagcgctg
     7141 cagcagggag ctccgggaca cttgaactgc cgtctaccct gtctctctac aagtccggag
     7201 cactggacga ggcagctgcg taccagagtc gcgactacta caactttcca ctggctctgg
     7261 ccggaccgcc gccccctccg ccgcctcccc atccccacgc tcgcatcaag ctggagaacc
     7321 cgctggacta cggcagcgcc tgggcggctg cggcggcgca gtgccgctat ggggacctgg
     7381 cgagcctgca tggcgcgggt gcagcgggac ccggttctgg gtcaccctca gccgccgctt
     7441 cctcatcctg gcacactctc ttcacagccg aagaaggcca gttgtatgga ccgtgtggtg
     7501 gtggtggggg tggtgg
//
