LOCUS       sequence-175295-        1182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express MORF4L1 (iso2) with a FLAG tag at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)
FEATURES             Location/Qualifiers
     source          1..1182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      172..181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             181..204
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa ggccgccatg
      181 gattacaagg atgatgatga taagcttatg gcgccgaagc aggacccgaa gcctaaattc
      241 caggagggtg agcgagtgct gtgctttcat gggcctcttc tttatgaagc aaagtgtgta
      301 aaggttgcca taaaggacaa acaagtgaaa tacttcatac attacagtgg ttggaataaa
      361 aattgggatg aatgggttcc ggagagcaga gtactcaaat acgtggacac caatttgcag
      421 aaacagcgag aacttcaaaa agccaatcag gagcagtatg cagaggggaa gatgagaggg
      481 gctgccccag gaaagaagac atctggtctg caacagaaaa atgttgaagt gaaaacgaaa
      541 aagaacaaac agaaaacacc tggaaatgga gatggtggca gtaccagtga gacccctcag
      601 cctcctcgga agaaaagggc ccgggtagat cctactgttg aaaatgagga aacattcatg
      661 aacagagttg aagttaaagt aaagattcct gaagagctaa aaccgtggct tgttgatgac
      721 tgggacttaa ttaccaggca aaaacagctc ttttatcttc ctgccaagaa gaatgtggat
      781 tccattcttg aggattatgc aaattacaag aaatctcgtg gaaacacaga taataaggag
      841 tatgcggtta atgaagttgt ggcagggata aaagaatact tcaacgtaat gttgggtacc
      901 cagctactct ataaatttga gagaccacag tatgctgaaa ttcttgcaga tcatcccgat
      961 gcacccatgt cccaggtgta tggagcgcca catctcctga gattatttgt acgaattgga
     1021 gcaatgttgg cttatacacc tctggatgag aagagccttg ctttattact caattatctt
     1081 cacgatttcc taaagtacct ggcaaagaat tctgcaactt tgttcagtgc cagcgattat
     1141 gaagtggctc ctcctgagta ccatcggaaa gctgtgtgag ga
//
