LOCUS       sequence-175695-       11119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP 
            CRISPR system. Use with pADH100-series gRNA expression constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Nguyen N, Quail MMF, Hernday AD
  TITLE     An Efficient, Rapid, and Recyclable System for CRISPR-Mediated 
            Genome Editing in Candida albicans.
  JOURNAL   mSphere. 2017 Apr 26;2(2):e00149-17. doi: 
            10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr.
  PUBMED    28497115
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/mSphereDirect.00149-17"; journalName: "mSphere"; date: 
            "2017-04-26- 26"; volume: "2"; issue: "2"; pages: "e00149-17"
COMMENT     Sequence Label: pADH99
FEATURES             Location/Qualifiers
     source          1..11119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..56
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    complement(523..556)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             1340..5443
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1340..5443
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5456..5476
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5480..5500
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5504..5524
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5525..5590
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      5655..5902
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5671..5689)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             6461..7732
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     promoter        8160..8503
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(join(9011..9017,9018..9035,9036..9041))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9056..9077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9194..9211)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9365..9953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9445..9464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10124..10915,10916..10984))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10747..10766
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10985..11089)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tgacggcacg gccacgcgtt taaaccgcct caagccatgg gatatgctca aaatattgct
      121 taactggggg tttaacaaat acatgtatgg taatccaaat gggtatgggg ttagtagtaa
      181 tttggtagtg aaaaccccgg tagacggaga attaataaat accccaaaat ccaccacagg
      241 actcgacagg tacctggagg atgaggagac agaagttagt agtaacaatt tggggacaga
      301 agaggataca gtgacatcag aggaagaaga cagcgaaggt atagtcaaaa agcaatagga
      361 cagcaagaca atttatttat ttaatagttt tttataaatg taaaatctat cacaacatgt
      421 gtacttaatt ttctgtggta ggggaggcca tttcgttgaa acgtgtcgcg agcggttgtg
      481 agaattgact cattttgatt attacgcaac cattgaaatt ttgaagttcc tatactttct
      541 agagaatagg aacttcacag ctaacaatta cacaaaaaaa attctctttt cgatgtagca
      601 actatcttca actaaaacat tttctccttc ggcccatgat tgtcctccgg gtcgacagca
      661 agccgttaca attgagatgg aaagcgacct accttcactc gataaggtgc ttaattgtac
      721 ttcatataaa tctggcccgg atctaaacaa atgagttcca ttaagccgtg ggttctcaat
      781 tagggttttt gtttttgatt tagaaaaaag agatcaagat ttgtttacag gtgatgcctt
      841 tttttagaac ttatgcgttg caaaagttga ctaacgattt ctataaggtg atccacacta
      901 attatacaaa cgtacaaaca gacatacttt tcctgcgttc acctgatgtt ggccagattt
      961 ctctcttcat tgcatagaac ataaccacac tagggcaaca gaaaaaaaaa aaaagtgcat
     1021 cgggaagttg tgttccattc attatatgtc tactactgca tatgagtagc ccacccacca
     1081 ccaccatagt aagtttttgt gtatgcgcgc cgtcaggtta tttcatttct gaatttttca
     1141 accaccttac tccctttatt gttgattgac aattttgctc acagtaagat cttttagact
     1201 ccaattaata taaaataagt ctgattttcc aattcctgtt ttttcttttt ttttctgttt
     1261 ctatttcttt ccttttctcc ctttttttta attcttcatt caatcatcaa ttgataattc
     1321 aggaatatta caacccggga tggataaaaa gtatagtatt ggtttagata ttggtactaa
     1381 ctctgtgggt tgggcagtta tcactgacga atataaagtt ccatcaaaga aatttaaggt
     1441 gttaggtaac actgacagac actcaataaa aaagaatctt atcggtgctc ttttgttcga
     1501 ctccggtgaa actgccgagg ctacacgttt aaaaagaaca gcaagaagaa gatatacccg
     1561 tagaaaaaat agaatatgtt atttacaaga aatcttttct aatgaaatgg ctaaagttga
     1621 tgattccttt ttccatagat tggaagagtc atttttggtt gaagaagaca aaaagcatga
     1681 gagacatcca atctttggga atatagttga tgaagtggct taccatgaaa aatatcctac
     1741 catttatcat ttaagaaaga aattggtaga ttcaactgat aaagctgacc ttagattaat
     1801 ctatttagca cttgcccata tgattaaatt tagaggtcat tttttgattg aaggtgattt
     1861 gaacccagat aattctgacg tggataaatt atttattcaa ttagtccaaa cctacaacca
     1921 attatttgag gaaaatccaa ttaatgctag tggtgtcgat gccaaagcta tattatcagc
     1981 cagattatca aaatctagac gtttggaaaa tttgattgcc caattgccag gagaaaaaaa
     2041 gaatggatta tttggaaact tgatcgcatt atcattgggt ttgacaccaa attttaaatc
     2101 taattttgat ttagctgaag atgctaaatt acaattatca aaagacacct atgacgacga
     2161 tttggacaat ttacttgctc aaattggtga tcaatatgca gatttgttct tagctgctaa
     2221 aaacttatct gatgctattt tgttgtctga tattttgaga gtgaacacag aaataaccaa
     2281 agctccatta tcagcatcta tgatcaaacg ttatgatgaa caccatcagg atttgacttt
     2341 attgaaagct ttggtgagac aacaattgcc agagaagtat aaagaaatct ttttcgatca
     2401 atctaaaaac gggtatgcag gttatattga tgggggtgcc tcccaagagg aattttacaa
     2461 atttataaaa cctattttag aaaagatgga tgggactgag gaacttttgg tcaaattgaa
     2521 cagagaagat ttgttacgta aacagagaac ttttgataat ggtagtatac ctcaccaaat
     2581 tcatttgggt gagttgcatg caattttaag aagacaagaa gatttttatc catttttaaa
     2641 agataataga gaaaaaatcg agaaaatttt aacctttaga attccatact atgttgggcc
     2701 tttggctaga ggtaattcaa gatttgcctg gatgacacgt aaatcagaag aaactattac
     2761 cccttggaat tttgaagagg ttgttgataa aggagcatca gcacagagtt ttattgaaag
     2821 aatgaccaat ttcgataaaa acttaccaaa tgaaaaagtt ttaccaaaac attccttgtt
     2881 atacgaatat tttactgttt acaatgaact tacaaaggtt aaatatgtta ctgaaggtat
     2941 gcgtaagcca gcctttttat ctggagaaca gaaaaaggca atagttgatt tattgtttaa
     3001 aacaaataga aaagttactg ttaaacaatt aaaagaagat tactttaaga aaattgaatg
     3061 ttttgattca gttgaaatca gtggtgttga agacagattt aatgctagtt taggaactta
     3121 ccatgattta cttaaaatta tcaaagataa agatttcttg gataacgaag aaaatgaaga
     3181 cattttagaa gacattgttt taaccttaac tttattcgaa gatagagaga tgattgaaga
     3241 acgtttgaag acttatgcac atttgtttga cgataaagtg atgaaacagt tgaaaagaag
     3301 acgttatact ggatggggta gattgtctcg taaattgatc aatggaatta gagataaaca
     3361 aagtggtaaa actatcttgg actttttgaa atctgacgga tttgctaata gaaatttcat
     3421 gcaattgatc cacgacgata gtttgacatt taaagaagac atccaaaagg cccaagtgag
     3481 tgggcaaggt gattcattac atgaacatat tgcaaattta gccggatctc ctgctattaa
     3541 gaaagggata ttacaaactg ttaaagttgt ggatgaatta gtgaaagtaa tgggaagaca
     3601 taaacctgaa aacattgtca ttgagatggc aagagaaaat caaactacac aaaaaggaca
     3661 gaaaaatagt agagaacgta tgaaaagaat agaagagggt attaaagaat tgggtagtca
     3721 aatattgaaa gaacacccag tggaaaatac ccagttgcaa aatgaaaaat tatatcttta
     3781 ctaccttcaa aatggacgtg atatgtatgt tgatcaggaa ttagatataa atagactttc
     3841 agattatgat gtagatcata tagttccaca atctttcttg aaagatgatt ccatagacaa
     3901 taaagtatta actagaagtg ataaaaatag aggtaaaagt gataatgtcc caagtgagga
     3961 agtcgtcaaa aagatgaaaa attactggcg tcaacttttg aatgctaaat taattactca
     4021 aagaaaattt gataatttga ctaaagcaga aagaggtggg ctttctgaat tagataaagc
     4081 cgggttcatt aaaagacaat tggtcgaaac tagacaaatt actaaacatg ttgcccaaat
     4141 tttagattcc cgtatgaaca ctaagtatga cgaaaatgat aagttaatac gtgaggttaa
     4201 agtcattact ttaaaatcaa aacttgtctc tgatttcaga aaggatttcc aattctataa
     4261 agttagagaa attaataatt atcatcatgc tcatgatgca tatttgaatg ctgtagttgg
     4321 aactgcttta atcaagaaat accctaaatt agaatctgaa tttgtatatg gtgattacaa
     4381 agtctatgat gttagaaaga tgattgctaa atcagaacaa gaaattggta aagctacagc
     4441 taaatacttc ttttactcta acattatgaa tttctttaaa acagaaatta ctttggcaaa
     4501 cggtgaaatt agaaaaagac ctcttattga aacaaatggt gagactggag agatagtttg
     4561 ggacaaaggg cgtgatttcg ctactgttag aaaagtttta tcaatgccac aagttaacat
     4621 tgtaaagaaa acagaggttc aaactggtgg tttctcaaaa gaaagtattt tgcctaaaag
     4681 aaatagtgat aaattgattg ccagaaaaaa ggattgggat ccaaagaaat atggtggttt
     4741 cgactcacca accgtagcct attctgtttt ggttgtggca aaggttgaaa agggtaaaag
     4801 taaaaagctt aaatcagtaa aagaactttt gggtattaca ataatggaaa gaagttcctt
     4861 tgaaaagaac cctattgatt ttttggaagc taaaggttat aaggaagtaa agaaggactt
     4921 aataatcaaa ttgcctaaat attctttatt tgaattagaa aatgggagaa aaagaatgtt
     4981 ggcttctgct ggagaattgc aaaagggtaa tgaattagca ttgccttcca aatatgttaa
     5041 cttcttgtat ttagcttcac actatgaaaa gttgaaaggg tcaccagaag ataacgagca
     5101 aaaacaatta tttgttgaac aacacaaaca ctacttagat gagattatag aacaaattag
     5161 tgaattcagt aaaagagtga tattagctga tgcaaattta gataaagttt tgtcagccta
     5221 taacaaacat agagataagc caattagaga acaagcagaa aacattattc acttatttac
     5281 ccttaccaat ttaggagcac ctgctgcttt caagtatttt gatacaacaa ttgatcgtaa
     5341 aagatatacc tcaacaaaag aagtcttaga cgccacctta attcatcaat caatcactgg
     5401 attgtatgag acaagaattg atttgtctca attgggtggt gatgaagggg ctgatcctaa
     5461 gaagaaaaga aaagttgatc caaagaaaaa gcgtaaggtg gatcctaaga aaaagagaaa
     5521 ggttgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     5581 cgatgacaag tgataatgac tgcagagatc catcgacctg ccgccaagct aattccgggc
     5641 gaatttctgt cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac
     5701 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt
     5761 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
     5821 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
     5881 acgctcgaag gctttaattt gcggccccaa taatgattgg tttgatattt ttgtctagta
     5941 ccatctgtac cattacactt aaattatctt tatatctgtc taactcgact gtctggattt
     6001 cattgatgta gtcgtatgca tcgttagttc caaaaaatat tgtcatcaat ttgatattgg
     6061 tttccgactc taaaattttt ggaagaattt gtctagcgtg ctctgagttg tagccactga
     6121 aaccacggtt aataacatcc aattttcgga tatacacatt ctgtaatgct ggatgaaagc
     6181 catactgggt acaactaaac tgggtgatgg agtcaccgaa caacacaaat ttaccgtatt
     6241 ccatgattgc tatggttgag aatttttttt ttttcttgtc ccacgccatt tttcaaatta
     6301 tgcagttgag aatgttagtt tttgtgtaca ccccgttcgc tgaatatttc ggaataattc
     6361 aaagattggg gagtggggga ggcgatagac gaagacacgg tataaaaatg ggcaaaattt
     6421 tccccaactt tttgcagtgg tttaactaat aatcgtcgac atgccacaat ttgatatatt
     6481 atgtaaaaca ccacctaagg tgcttgttcg tcagtttgtg gaaaggtttg aaagaccttc
     6541 aggtgagaaa atagcattat gtgctgctga actaacctat ttatgttgga tgattacaca
     6601 taacggaaca gcaatcaaga gagccacatt catgagctat aatactatca taagcaattc
     6661 gttgagtttc gatattgtca ataaatcact ccagtttaaa tacaagacgc aaaaagcaac
     6721 aattttggaa gcctcattaa agaaattgat tcctgcttgg gaatttacaa ttattcctta
     6781 ctatggacaa aaacatcaat ctgatatcac tgatattgta agtagtttgc aattacagtt
     6841 cgaatcatcg gaagaagcag ataagggaaa tagccacagt aaaaaaatgc ttaaagcact
     6901 tctaagtgag ggtgaaagca tctgggagat cactgagaaa atactaaatt cgtttgagta
     6961 tacttcgaga tttacaaaaa caaaaacttt ataccaattc ctcttcctag ctactttcat
     7021 caattgtgga agattcagcg atattaagaa cgttgatccg aaatcattta aattagtcca
     7081 aaataagtat ttgggagtaa taatccagtg tttagtgaca gagacaaaga caagcgttag
     7141 taggcacata tacttcttta gcgcaagggg taggatcgat ccacttgtat atttggatga
     7201 atttttgagg aattctgaac cagtcctaaa acgagtaaat aggaccggca attcttcaag
     7261 caataaacag gaataccaat tattaaaaga taacttagtc agatcgtaca ataaagcttt
     7321 gaagaaaaat gcgccttatt caatctttgc tataaaaaat ggcccaaaat ctcacattgg
     7381 aagacatttg atgacctcat ttctttcaat gaagggccta acggagttga ctaatgttgt
     7441 gggaaattgg agcgataagc gtgcttctgc cgtggccagg acaacgtata ctcatcagat
     7501 aacagcaata cctgatcact acttcgcact agtttctcgg tactatgcat atgatccaat
     7561 atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt ggcagcatat
     7621 agaacagcta aagggtagtg ctgaaggaag catacgatac cccgcatgga atgggataat
     7681 atcacaggag gtactagact acctttcatc ctacataaat agacgcatat aagagtgaaa
     7741 ttctggaaat ctggaaatct ggttttgtat tcttgttatt cttctttttg ttattacata
     7801 tataacttgt tactttttta aaaaaatctt tgtatatttt ataaatatat aaaactaaat
     7861 ttaagaaaaa gagaaaaatg ttttatttga gagattgaaa ttttacttga atttagctta
     7921 gcttttataa agtattatta tgtaaaaaaa caaaacaaat atacattaaa aagttaagac
     7981 tataaaatag ccacccaagg catttctata tcttgttgtt gttgttttca tcttctgtat
     8041 cagaggaact tattttatta ttttcgtcac gggtattttc tcttgtttga tgattcatcc
     8101 cattcattcc atcataaaat atcaagcttg cctcgtcccc gccgggtcac ccggccagcg
     8161 acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat
     8221 gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac
     8281 attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc
     8341 agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa
     8401 atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct
     8461 tgctaggata cagttctcac atcacatccg aacataaaca accggatcct acaattatgt
     8521 ctactacttt ggatgatact gcttatagat acagaacttc tgttccaggt gatgctgaag
     8581 ctattgaagc tttggatggt tctttcacta ccgatactgt ttttagagtt actgctactg
     8641 gtgatggttt cactttgaga gaagttccag ttgatccacc attgactaag gttttcccag
     8701 atgatgaatc cgatgatgaa tccgatgctg gtgaagatgg tgatccagat tctagaactt
     8761 tcgttgctta tggtgatgat ggtgatttgg ctggtttcgt tgttgtttct tattctggtt
     8821 ggaacagaag attgactgtt gaagatattg aagttgctcc agaacataga ggtcatggtg
     8881 ttggtagagc tttgatgggt ttggctactg aattcgccag agaaagaggt gctggtcatt
     8941 tgtggttgga agttaccaat gttaatcggt gtttaaaccc cagcgcctgg cgggcgctca
     9001 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     9061 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9121 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     9181 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9241 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     9361 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     9421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9481 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9541 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9601 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9661 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9721 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9781 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     9841 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9901 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9961 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    10021 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    10081 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10141 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10201 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10261 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10321 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10381 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    10441 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10501 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10561 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10621 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10681 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10741 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10801 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10861 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10921 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10981 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    11041 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    11101 gaaaagtgcc acctgacgt
//
