LOCUS       sequence-175697-        5584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5584)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Cx32-7
FEATURES             Location/Qualifiers
     source          1..5584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             871..1551
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     polyA_signal    1676..1797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1713..1732)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1767..1786
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1804..2259)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1941..1962)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2153..2172
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2286..2390
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2392..2749
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2392..2583
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2415..2435)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2600..2735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2784..3578
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2784..3578
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2838..2857)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3448..3467
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3766..3785)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3810..3857
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4186..4774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4675..4694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4949..5252
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5253..5456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5407..5427
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgaactgga caggtttgta caccttgctc agtggcgtga accggcattc tactgccatt
       61 ggccgagtat ggctctcggt catcttcatc ttcagaatca tggtgctggt ggtggctgca
      121 gagagtgtgt ggggtgatga gaaatcttcc ttcatctgca acacactcca gcctggctgc
      181 aacagcgttt gctatgacca attcttcccc atctcccatg tgcggctgtg gtccctgcag
      241 ctcatcctag tttccacccc agctctcctc gtggccatgc acgtggctca ccagcaacac
      301 atagagaaga aaatgctacg gcttgagggc catggggacc ccctacacct ggaggaggtg
      361 aagaggcaca aggtccacat ctcagggaca ctgtggtgga cctatgtcat cagcgtggtg
      421 ttccggctgt tgtttgaggc cgtcttcatg tatgtctttt atctgctcta ccctggctat
      481 gccatggtgc ggctggtcaa gtgcgacgtc tacccctgcc ccaacacagt ggactgcttc
      541 gtgtcccgcc ccaccgagaa aaccgtcttc accgtcttca tgctagctgc ctctggcatc
      601 tgcatcatcc tcaatgtggc cgaggtggtg tacctcatca tccgggcctg tgcccgccga
      661 gcccagcgcc gctccaatcc accttcccgc aagggctcgg gcttcggcca ccgcctctca
      721 cctgaataca agcagaatga gatcaacaag ctgctgagtg agcaggatgg ctccctgaaa
      781 gacatactgc gccgcagccc tggcaccggg gctgggctgg ctgaaaagag cgaccgctgc
      841 tcggcctgcg cggatccacc ggtcgccacc atgaacagcc tgatcaaaga aaacatgcgg
      901 atgaaggtgg tgctggaagg cagcgtgaac ggccaccagt tcaagtgcac cggcgagggc
      961 gagggcaacc cctacatggg cacccagacc atgcggatca aagtgatcga gggcggacct
     1021 ctgcccttcg ccttcgacat cctggccaca tccttcatgt acggcagccg gaccttcatc
     1081 aagtacccca agggcatccc cgatttcttc aagcagagct tccccgaggg cttcacctgg
     1141 gagagagtga ccagatacga ggacggcggc gtgatcaccg tgatgcagga caccagcctg
     1201 gaagatggct gcctggtgta ccatgcccag gtcaggggcg tgaattttcc cagcaacggc
     1261 gccgtgatgc agaagaaaac caagggctgg gagcccaaca ccgagatgat gtaccccgct
     1321 gacggcggac tgagaggcta cacccacatg gccctgaagg tggacggcgg agggcacctg
     1381 agctgcagct tcgtgaccac ctaccgatcc aagaaaaccg tgggcaacat caagatgccc
     1441 ggcatccacg ccgtggacca ccggctggaa aggctggaag agtccgacaa cgagatgttc
     1501 gtggtgcagc gggagcacgc cgtggccaag ttcgccggcc tgggcggagg gtaagcggcc
     1561 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     1621 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     1681 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1741 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     1801 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     1861 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     1921 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     1981 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     2041 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     2101 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     2161 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     2221 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     2281 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2341 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     2401 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     2461 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     2521 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     2581 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     2641 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     2701 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     2761 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     2821 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     2881 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     2941 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     3001 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3061 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     3121 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     3181 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     3241 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     3301 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     3361 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     3421 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     3481 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     3541 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     3601 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     3661 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     3721 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     3781 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     3841 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     3901 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     3961 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     4021 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4081 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4141 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4201 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4261 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4321 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4381 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4441 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4501 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4561 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4621 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4681 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4741 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4801 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4861 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
     4921 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     4981 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     5041 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     5101 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     5161 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     5221 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     5281 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5341 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     5401 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     5461 tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat
     5521 tccctctggg aaagggcagc agcagccagg tgtggcagtg acagggaggt gtgaatgagg
     5581 cagg
//
