LOCUS       sequence-175729-        9138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full-length human progesterone receptor-A, 
            equivalent to human PR-B amino acids 165-933.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Su S, Blackwelder AJ, Grossman G, Minges JT, Yuan L, Young SL, 
            Wilson EM
  TITLE     Primate-specific melanoma antigen-A11 regulates isoform-specific 
            human progesterone receptor-B transactivation.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 
            10.1074/jbc.M112.372797. Epub 2012 Aug 13.
  PUBMED    22891251
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.372797"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34809-24"
COMMENT     Sequence Label: pcDNA3-hPR-A
FEATURES             Location/Qualifiers
     source          1..9138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4372..4383
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(4457..4475)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4458..4475)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4495..4512)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4501..4725
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4771..5199
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4858..4877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5068..5089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5208..5228)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5213..5542
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5393..5528
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5609..6403
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5663..5682)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6273..6292
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6577..6698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6614..6633)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6668..6687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6747..6763)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6747..6763)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6760..6782)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6771..6787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6795..6801,6802..6819,6820..6825))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6840..6861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6978..6995)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7149..7737)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7229..7248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7908..8699,8700..8768))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8531..8550
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8769..8873)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8948..8967)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatcccc actgctgtgt cgcccagccg caggtccgtt cccggggagc
      721 cagacctcgg acaccttgcc tgaagtttcg gccataccta tctccctgga cgggctactc
      781 ttccctcggc cctgccaggg acaggacccc tccgacgaaa agacgcagga ccagcagtcg
      841 ctgtcggacg tggagggcgc atattccaga gctgaagcta caaggggtgc tggaggcagc
      901 agttctagtc ccccagaaaa ggacagcgga ctgctggaca gtgtcttgga cactctgttg
      961 gcgccctcag gtcccgggca gagccaaccc agccctcccg cctgcgaggt caccagctct
     1021 tggtgcctgt ttggccccga acttcccgaa gatccaccgg ctgcccccgc cacccagcgg
     1081 gtgttgtccc cgctcatgag ccggtccggg tgcaaggttg gagacagctc cgggacggca
     1141 gctgcccata aagtgctgcc ccggggcctg tcaccagccc ggcagctgct gctcccggcc
     1201 tctgagagcc ctcactggtc cggggcccca gtgaagccgt ctccgcaggc cgctgcggtg
     1261 gaggttgagg aggaggatgg ctctgagtcc gaggagtctg cgggtccgct tctgaagggc
     1321 aaacctcggg ctctgggtgg cgcggcggct ggaggaggag ccgcggctgt cccgccgggg
     1381 gcggcagcag gaggcgtcgc cctggtcccc aaggaagatt cccgcttctc agcgcccagg
     1441 gtcgccctgg tggagcagga cgcgccgatg gcgcccgggc gctccccgct ggccaccacg
     1501 gtgatggatt tcatccacgt gcctatcctg cctctcaatc acgccttatt ggcagcccgc
     1561 actcggcagc tgctggaaga cgaaagttac gacggcgggg ccggggctgc cagcgccttt
     1621 gccccgccgc ggagttcacc ctgtgcctcg tccaccccgg tcgctgtagg cgacttcccc
     1681 gactgcgcgt acccgcccga cgccgagccc aaggacgacg cgtaccctct ctatagcgac
     1741 ttccagccgc ccgctctaaa gataaaggag gaggaggaag gtgcggaggc ctccgcgcgc
     1801 tccccgcgtt cctaccttgt ggccggtgcc aaccccgcag ccttcccgga tttcccgttg
     1861 gggccaccgc ccccgctgcc gccgcgagcg accccatcca gacccgggga agcggcggtg
     1921 acggccgcac ccgccagtgc ctcagtctcg tctgcgtcct cctcggggtc gaccctggag
     1981 tgcatcctgt acaaagcgga gggcgcgccg ccccagcagg gcccgttcgc gccgccgccc
     2041 tgcaaggcgc cgggcgcgag cggctgcctg ctcccgcggg acggcctgcc ctccacctcc
     2101 gcctctgccg ccgccgccgg ggcggccccc gcgctctacc ctgcactcgg cctcaacggg
     2161 ctcccgcagc tcggctacca ggccgccgtg ctcaaggagg gcctgccgca ggtctacccg
     2221 ccctatctca actacctgag gccggattca gaagccagcc agagcccaca atacagcttc
     2281 gagtcattac ctcagaagat ttgtttaatc tgtggggatg aagcatcagg ctgtcattat
     2341 ggtgtcctta cctgtgggag ctgtaaggtc ttctttaaga gggcaatgga agggcagcac
     2401 aactacttat gtgctggaag aaatgactgc atcgttgata aaatccgcag aaaaaactgc
     2461 ccagcatgtc gccttagaaa gtgctgtcag gctggcatgg tccttggagg tcgaaaattt
     2521 aaaaagttca ataaagtcag agttgtgaga gcactggatg ctgttgctct cccacagcca
     2581 ttgggcgttc caaatgaaag ccaagcccta agccagagat tcactttttc accaggtcaa
     2641 gacatacagt tgattccacc actgatcaac ctgttaatga gcattgaacc agatgtgatc
     2701 tatgcaggac atgacaacac aaaacctgac acctccagtt ctttgctgac aagtcttaat
     2761 caactaggcg agaggcaact tctttcagta gtcaagtggt ctaaatcatt gccaggtttt
     2821 cgaaacttac atattgatga ccagataact ctcattcagt attcttggat gagcttaatg
     2881 gtgtttggtc taggatggag atcctacaaa catgtcagtg ggcagatgct gtattttgca
     2941 cctgatctaa tactaaatga acagcggatg aaagaatcat cattctattc attatgcctt
     3001 accatgtggc agatcccaca ggagtttgtc aagcttcaag ttagccaaga agagttcctc
     3061 tgtatgaaag tattgttact tcttaataca attcctttgg aagggctacg aagtcaaacc
     3121 cagtttgagg agatgaggtc aagctacatt agagagctca tcaaggcaat tggtttgagg
     3181 caaaaaggag ttgtgtcgag ctcacagcgt ttctatcaac ttacaaaact tcttgataac
     3241 ttgcatgatc ttgtcaaaca gcttcatctg tactgcttga atacatttat ccagtcccgg
     3301 gcactgagtg ttgaatttcc agaaatgatg tctgaagtta ttgctgcaca attacccaag
     3361 atattggcag ggatggtgaa accccttctc tttcataaaa agtgaatgtc atctttttct
     3421 tttaaagaat taaattttgt ggcatgtctt tttgttttgg tcaggattat gaggtcttga
     3481 gtttttataa tgttcttctg aaagccttac atttataaca tcatagtgtg taaatttaaa
     3541 agaaaaattg tgaggttcta attattttct tttataaagt ataattagaa tgtttaactg
     3601 ttttgtttac ccatattttc ttgaagaatt tacaagattg aaaaagtact aaaattgtta
     3661 aagtaaacta tatcttatcc atattatttc ataccatgta ggtgaggatt tttaactttt
     3721 gcatctaaca aatcatcgac ttaagagaaa aaatcttaca tgtaataaca caaagctatt
     3781 atatgttatt tctaggtaac tccctttgtg tcaattatat ttccaaaaat gaacctttaa
     3841 aatggtatgc aaaattttgt ctatatatat ttgtgtgagg aggaaattca taactttcct
     3901 cagattttca aaagtatttt taatgcaaaa aatgtagaaa gagtttaaaa ccactaaaat
     3961 agattgatgt tcttcaaact aggcaaaaca actcatatgt taagaccatt ttccagattg
     4021 gaaacacaaa tctcttagga agttaataag tagattcata tcattataca aatagtattg
     4081 tgggttttgt aggtttttaa aataaccttt tttggggaga gaattgtcct ctaatgaggt
     4141 attgcgagtg gacataagaa atcagaagat tatggcctaa ctgtactcct taccaactgt
     4201 ggcatgctga aagttagtca ctcttactga ttctcaattc tctcaccttt gaaagtagta
     4261 aaatatcttt cctgccaatt gctcctttgg gtcagagctt attaacatct tttcaaatca
     4321 aaggaaagaa gaaagggaga ggaggaggag ggagatatca attcacatac ctttctcctc
     4381 tttatcctcc actatcatga attcagatca tccatcacac tggcggccgc tcgagcatgc
     4441 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     4501 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4561 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4621 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4681 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4741 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4801 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4981 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5101 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5161 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5221 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5281 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5341 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5401 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5461 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5521 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     5581 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5641 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5701 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5761 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5821 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5881 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5941 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6001 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6061 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6121 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6181 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6241 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6301 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6361 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6421 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6481 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6541 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6601 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6661 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6721 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6781 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6841 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6901 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6961 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7021 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7081 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7141 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7201 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7261 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7321 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7381 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7441 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7501 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7561 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7621 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7681 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7741 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7801 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7861 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7921 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7981 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8041 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8101 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8161 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8221 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8281 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8341 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8401 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8461 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8521 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8581 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8641 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8701 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8761 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8821 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8881 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     8941 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     9001 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     9061 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     9121 gggccagata tacgcgtt
//
