LOCUS       sequence-17606-e         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Ramaswamy S, Nakamura N, Vazquez F, Batt DB, Perera S, Roberts TM, 
            Sellers WR
  TITLE     Regulation of G1 progression by the PTEN tumor suppressor protein is
            linked to inhibition of the phosphatidylinositol 3-kinase/Akt 
            pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5.
  PUBMED    10051603
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Mar 2. 96(5):2110-5."
COMMENT     Sequence Label: 1017 pcDNA3 Myr Akt1 deltaPH
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..151
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 ctctctggct actagagaaa cccactgtct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagcttg tcgacggtac cccggggtaa ttcgccgcca tggggagtag
      121 caagagcaag cctaaggacc ccagccagcg cagcgaggct gaagagatgg aggtgtccct
      181 ggccaagccc aagcaccgcg tgaccatgaa cgagtttgag tacctgaagc tgctgggcaa
      241 gggcactttc ggcaaggtga tcctggtgaa ggagaaggcc acaggccgct actacgccat
      301 gaagatcctc aagaaggaag tcatcgtggc caaggacgag gtggcccaca cactcaccga
      361 gaaccgcgtc ctgcagaact ccaggcaccc cttcctcaca gccctgaagt actctttcca
      421 gacccacgac cgcctctgct ttgtcatgga gtacgccaac gggggcgagc tgttcttcca
      481 cctgtcccgg gaacgtgtgt tctccgagga ccgggcccgc ttctatggcg ctgagattgt
      541 gtcagccctg gactacctgc actcggagaa
//
