LOCUS       sequence-176077-        9201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full-length human progesterone receptor-B in
            pcDNA3, can be used to make stable cell line, select G418.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Su S, Blackwelder AJ, Grossman G, Minges JT, Yuan L, Young SL, 
            Wilson EM
  TITLE     Primate-specific melanoma antigen-A11 regulates isoform-specific 
            human progesterone receptor-B transactivation.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 
            10.1074/jbc.M112.372797. Epub 2012 Aug 13.
  PUBMED    22891251
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.372797"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34809-24"
COMMENT     Sequence Label: pcDNA3-PRB
FEATURES             Location/Qualifiers
     source          1..9201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4435..4446
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(4520..4538)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4521..4538)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4558..4575)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4564..4788
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4834..5262
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4921..4940)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5131..5152
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5271..5291)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5276..5605
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5456..5591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5672..6466
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5726..5745)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6336..6355
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6640..6761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6677..6696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6731..6750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6810..6826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6810..6826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6823..6845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6834..6850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6858..6864,6865..6882,6883..6888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6903..6924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7041..7058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7212..7800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7292..7311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7971..8762,8763..8831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8594..8613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8832..8936)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9011..9030)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccatga ctgagctgaa ggcaaagggt ccccgggctc cccacgtggc gggcggcccg
      721 ccctcccccg aggtcggatc cccactgctg tgtcgcccag ccgcaggtcc gttcccgggg
      781 agccagacct cggacacctt gcctgaagtt tcggccatac ctatctccct ggacgggcta
      841 ctcttccctc ggccctgcca gggacaggac ccctccgacg aaaagacgca ggaccagcag
      901 tcgctgtcgg acgtggaggg cgcatattcc agagctgaag ctacaagggg tgctggaggc
      961 agcagttcta gtcccccaga aaaggacagc ggactgctgg acagtgtctt ggacactctg
     1021 ttggcgccct caggtcccgg gcagagccaa cccagccctc ccgcctgcga ggtcaccagc
     1081 tcttggtgcc tgtttggccc cgaacttccc gaagatccac cggctgcccc cgccacccag
     1141 cgggtgttgt ccccgctcat gagccggtcc gggtgcaagg ttggagacag ctccgggacg
     1201 gcagctgccc ataaagtgct gccccggggc ctgtcaccag cccggcagct gctgctcccg
     1261 gcctctgaga gccctcactg gtccggggcc ccagtgaagc cgtctccgca ggccgctgcg
     1321 gtggaggttg aggaggagga tggctctgag tccgaggagt ctgcgggtcc gcttctgaag
     1381 ggcaaacctc gggctctggg tggcgcggcg gctggaggag gagccgcggc tgtcccgccg
     1441 ggggcggcag caggaggcgt cgccctggtc cccaaggaag attcccgctt ctcagcgccc
     1501 agggtcgccc tggtggagca ggacgcgccg atggcgcccg ggcgctcccc gctggccacc
     1561 acggtgatgg atttcatcca cgtgcctatc ctgcctctca atcacgcctt attggcagcc
     1621 cgcactcggc agctgctgga agacgaaagt tacgacggcg gggccggggc tgccagcgcc
     1681 tttgccccgc cgcggagttc accctgtgcc tcgtccaccc cggtcgctgt aggcgacttc
     1741 cccgactgcg cgtacccgcc cgacgccgag cccaaggacg acgcgtaccc tctctatagc
     1801 gacttccagc cgcccgctct aaagataaag gaggaggagg aaggtgcgga ggcctccgcg
     1861 cgctccccgc gttcctacct tgtggccggt gccaaccccg cagccttccc ggatttcccg
     1921 ttggggccac cgcccccgct gccgccgcga gcgaccccat ccagacccgg ggaagcggcg
     1981 gtgacggccg cacccgccag tgcctcagtc tcgtctgcgt cctcctcggg gtcgaccctg
     2041 gagtgcatcc tgtacaaagc ggagggcgcg ccgccccagc agggcccgtt cgcgccgccg
     2101 ccctgcaagg cgccgggcgc gagcggctgc ctgctcccgc gggacggcct gccctccacc
     2161 tccgcctctg ccgccgccgc cggggcggcc cccgcgctct accctgcact cggcctcaac
     2221 gggctcccgc agctcggcta ccaggccgcc gtgctcaagg agggcctgcc gcaggtctac
     2281 ccgccctatc tcaactacct gaggccggat tcagaagcca gccagagccc acaatacagc
     2341 ttcgagtcat tacctcagaa gatttgttta atctgtgggg atgaagcatc aggctgtcat
     2401 tatggtgtcc ttacctgtgg gagctgtaag gtcttcttta agagggcaat ggaagggcag
     2461 cacaactact tatgtgctgg aagaaatgac tgcatcgttg ataaaatccg cagaaaaaac
     2521 tgcccagcat gtcgccttag aaagtgctgt caggctggca tggtccttgg aggtcgaaaa
     2581 tttaaaaagt tcaataaagt cagagttgtg agagcactgg atgctgttgc tctcccacag
     2641 ccattgggcg ttccaaatga aagccaagcc ctaagccaga gattcacttt ttcaccaggt
     2701 caagacatac agttgattcc accactgatc aacctgttaa tgagcattga accagatgtg
     2761 atctatgcag gacatgacaa cacaaaacct gacacctcca gttctttgct gacaagtctt
     2821 aatcaactag gcgagaggca acttctttca gtagtcaagt ggtctaaatc attgccaggt
     2881 tttcgaaact tacatattga tgaccagata actctcattc agtattcttg gatgagctta
     2941 atggtgtttg gtctaggatg gagatcctac aaacatgtca gtgggcagat gctgtatttt
     3001 gcacctgatc taatactaaa tgaacagcgg atgaaagaat catcattcta ttcattatgc
     3061 cttaccatgt ggcagatccc acaggagttt gtcaagcttc aagttagcca agaagagttc
     3121 ctctgtatga aagtattgtt acttcttaat acaattcctt tggaagggct acgaagtcaa
     3181 acccagtttg aggagatgag gtcaagctac attagagagc tcatcaaggc aattggtttg
     3241 aggcaaaaag gagttgtgtc gagctcacag cgtttctatc aacttacaaa acttcttgat
     3301 aacttgcatg atcttgtcaa acagcttcat ctgtactgct tgaatacatt tatccagtcc
     3361 cgggcactga gtgttgaatt tccagaaatg atgtctgaag ttattgctgc acaattaccc
     3421 aagatattgg cagggatggt gaaacccctt ctctttcata aaaagtgaat gtcatctttt
     3481 tcttttaaag aattaaattt tgtggcatgt ctttttgttt tggtcaggat tatgaggtct
     3541 tgagttttta taatgttctt ctgaaagcct tacatttata acatcatagt gtgtaaattt
     3601 aaaagaaaaa ttgtgaggtt ctaattattt tcttttataa agtataatta gaatgtttaa
     3661 ctgttttgtt tacccatatt ttcttgaaga atttacaaga ttgaaaaagt actaaaattg
     3721 ttaaagtaaa ctatatctta tccatattat ttcataccat gtaggtgagg atttttaact
     3781 tttgcatcta acaaatcatc gacttaagag aaaaaatctt acatgtaata acacaaagct
     3841 attatatgtt atttctaggt aactcccttt gtgtcaatta tatttccaaa aatgaacctt
     3901 taaaatggta tgcaaaattt tgtctatata tatttgtgtg aggaggaaat tcataacttt
     3961 cctcagattt tcaaaagtat ttttaatgca aaaaatgtag aaagagttta aaaccactaa
     4021 aatagattga tgttcttcaa actaggcaaa acaactcata tgttaagacc attttccaga
     4081 ttggaaacac aaatctctta ggaagttaat aagtagattc atatcattat acaaatagta
     4141 ttgtgggttt tgtaggtttt taaaataacc ttttttgggg agagaattgt cctctaatga
     4201 ggtattgcga gtggacataa gaaatcagaa gattatggcc taactgtact ccttaccaac
     4261 tgtggcatgc tgaaagttag tcactcttac tgattctcaa ttctctcacc tttgaaagta
     4321 gtaaaatatc tttcctgcca attgctcctt tgggtcagag cttattaaca tcttttcaaa
     4381 tcaaaggaaa gaagaaaggg agaggaggag gagggagata tcaattcaca tacctttctc
     4441 ctctttatcc tccactatca tgaattcaga tcatccatca cactggcggc cgctcgagca
     4501 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
     4561 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4621 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4681 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4741 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4801 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4861 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4921 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4981 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5041 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5101 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5161 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5221 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5281 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5341 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5401 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5461 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5521 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     5581 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     5641 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5701 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5761 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5821 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     5881 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5941 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6001 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6061 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6121 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6181 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6241 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6301 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6361 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6421 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6481 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6541 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6601 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6721 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6781 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6841 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6901 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6961 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7021 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7081 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7141 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7201 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7261 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7321 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7381 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7441 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7501 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7561 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7621 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7681 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7741 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7801 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7861 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7921 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7981 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8041 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8101 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8161 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8221 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8281 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8341 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8401 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8461 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8521 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8581 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8641 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8701 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8761 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8821 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8881 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8941 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     9001 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     9061 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     9121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     9181 tacgggccag atatacgcgt t
//
