LOCUS       sequence-176098-        4684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Rotavirus SA11 NSP1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, 
            Matsuura Y, Taniguchi K, Kobayashi T
  TITLE     Entirely plasmid-based reverse genetics system for rotaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114.
  PUBMED    28137864
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114."
COMMENT     Sequence Label: pT7-NSP1SA11
FEATURES             Location/Qualifiers
     source          1..4684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..36
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(74..96)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     196..215
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     425..444
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        734..752
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     734..751
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(820..867)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     863..881
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(2636..2654)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2739..2756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2910..3498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2990..3009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3669..4460,4461..4529))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4292..4311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4530..4634)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
       61 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
      121 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
      181 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat tcgacgctct
      241 cccttatgcg actcctgcat taagaagcag cccagtagta ggttgaggcc gttgagcacc
      301 gccgccgcaa ggaatggtgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
      361 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
      421 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
      481 ccacgatgcg tccggcgtag aggatctggc tagcgatgac cctgctgatt ggttcgctga
      541 ccatttccgg gtgcgggacg gcgttaccag aaactcagaa ggttcgtcca accaaaccga
      601 ctctgacggc agtttacgag agagatgata gggtctgctt cagtaagcca gatgctacac
      661 aattaggctt gtacatattg tcgttagaac gcggctacaa ttaatacata accttatgta
      721 tcatacacat acgatttagg tgacactata gaatacaagc ttgcatgcct gcaggtcgac
      781 tctagaggat ctcgatccgg atatagttcc tcctttcagc aaaaaacccc tcaagacccg
      841 tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag ccaactcagc
      901 ttcctttcgg gctttgttag cagccggatc cttttttttg agctctccct tagccatccg
      961 agtggacgac gtcctccttc ggatgcccag gtcggaccgc gaggaggtgg agatgccatg
     1021 ccgacccggt tcacagtatt ttgccagcta ggcgctactc tagtctggac ggtcttgtga
     1081 tggctaaata ctgtataatt agatagtgac ataatttcac tcattgtcat cttctgagtt
     1141 ggagatcagt agctcaaatt cttcagttaa agttccagaa ttcttcattt caatcagttc
     1201 ctccgttgat tttattgaca ttggcaattt atccaattca tcaacattat accatttact
     1261 gctaattgat ttaactatac tactgaattc atttaaatta agtaccattg gtatgctatc
     1321 tacattcaaa gtgcgcttaa tttcattata caaactaata ttcaatccaa gaatcaacat
     1381 gatgacattc gtaccttcaa ctgacactgg cgacatgtga ttaaacactt cttcaattgt
     1441 ttgatccatg tgattagtaa acaaactcca aattaatctt ttaattaaag gatatatgtt
     1501 ttcaacagaa ctacaatgtc caacgttaac attactttta taacaatcct taataaattc
     1561 catcaataaa ttatacatat cacgaatccg ccaatcaatt ttactaagaa aatattttat
     1621 attacaccat ttacagtact ggatatcgga agcattgcct gggtgattag atgccacgta
     1681 gttaggctga caatgatgtc ccaactcaaa tattgtcatt ttcaaccttc caaaattatt
     1741 atcatgagca tagaaccgag tatacttact cgtcatcatt gcagcaatta atttattctt
     1801 atcatttttt aaatcagaat aaagctccaa ttcatctctc caattaaatt cagttacatt
     1861 tctgtatatt gatcttcctg gcgtttttgt ataagtatta aaaatcacag tttctttagt
     1921 aaaatctgaa aagtaaattt gctttagttt tgatgatggc agtcttgata attggaagta
     1981 tttctttgcg taatggtttc tcaactctat tggtagattg gacattcttg taaagttaat
     2041 atgatcaagc aataatttat caaattcatt tataaagtta acaattctat atggagcata
     2101 tatatcactg actgatttct catataaccc aaaaacataa taaagattat tttcatcaaa
     2161 cttaaatgca gcagcattta atgtaattgg gagcagcaaa tgattatacc attcaatttt
     2221 atattgattc ctacattctc tttgctttat cattctttca aattttctaa ttactttatc
     2281 attaattgga aatattatat tgtacaagtc tatgatacac tgtaatttgt ctttggtaat
     2341 tggatgtttc acagttctaa gcttaagcaa atgaggttca ttgtcaagga agcatcttct
     2401 gttctgaaca caccatttgc aaacatgcgg caatgagcaa ccataacaat gggttaaatc
     2461 agctatttga caacattcta aacaccaccc cttaattttc gcatcaggaa ctggcatcca
     2521 aatagaattt gcaccaatgt tgcataatct ccgatttaaa gcagttaatc tacgataatg
     2581 aaagcatgca tctttaaaag tagccatggc taacacaaga cttttcaaaa aaaagcctat
     2641 agtgagtcgt attaacccgg gatcgatatc cccctgcatt aatgaatcgg ccaacgcgcg
     2701 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2761 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2821 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2881 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2941 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3001 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3061 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3121 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3181 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3241 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3301 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3361 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3421 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3481 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3541 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3601 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3661 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3721 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3781 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3841 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3901 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3961 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4021 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4081 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4141 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4201 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4261 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4321 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4381 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4441 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4501 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4561 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4621 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     4681 atca
//
