LOCUS       sequence-176294-        8639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one gene in combination with turbo GFP using the P2A self-cleaving 
            peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Barger CJ, Chee L, Albahrani M, Munoz-Trujillo C, Boghean L, Branick
            C, Odunsi K, Drapkin R, Zou L, Karpf AR
  TITLE     Co-regulation and function of FOXM1/RHNO1 bidirectional genes in 
            cancer.
  JOURNAL   Elife. 2021 Apr 23;10. pii: 55070. doi: 10.7554/eLife.55070.
  PUBMED    33890574
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-04-23"; volume: "10. pii: 55070. doi"; pages: " 
            10.7554/eLife.55070"
COMMENT     Sequence Label: pCW57-GFP-P2A-MCS (Neo)
FEATURES             Location/Qualifiers
     source          1..8639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    58..76
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    94..112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    129..147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    165..183
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    201..219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    236..254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    272..290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    352..469
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(682..726)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    911..1144
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1637..1762
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1809..1989
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1990..2216)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2244..2264)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2244..2262)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2283..2299)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2283..2299)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2296..2318)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2307..2323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2331..2337,2338..2355,2356..2361))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2376..2397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2514..2531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2685..3273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2765..2784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3444..4235,4236..4304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4067..4086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4305..4376)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4376..4511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4551..4672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4562..4581)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4616..4635
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4744..4977
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5182..5193
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5591..5611
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5652..6455)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSRMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5763..5782)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6373..6392
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(6456..6509)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(6525..6530,6531..6566,6567..6569,6570..6605,
                     6606..6608,6609..6644,6645..6650,6651..7268))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(7292..7802)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7355..7373)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(7830..7886)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7899..8594)
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     regulatory      8591..8600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(8623..8639,1..298))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(join(8626..8639,1..11))
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctaaacgagc tctgcttata taggcctccc accgtacacg cctacctcga catacgttct
       61 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gataaactcg
      121 acatacgttc tctatcactg atagggagta aactcgacat acgttctcta tcactgatag
      181 ggagtaaact cgacatacgt tctctatcac tgatagggag taaactcgac atcgttctct
      241 atcactgata gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcga
      301 catatcgatt cgcggccaaa gtggatctct gctgtccctg taataaaccc gaaaattttg
      361 aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac
      421 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa
      481 tactgccatt tgtctcgagg tcgagaattg tcccctcggg gttgggaggt gggtctgaaa
      541 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
      601 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
      661 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
      721 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
      781 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
      841 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
      901 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
      961 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     1021 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     1081 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     1141 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     1201 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     1261 cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac
     1321 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     1381 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     1441 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     1501 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     1561 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     1621 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     1681 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     1741 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     1801 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     1861 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     1921 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     1981 gagagacccg tttatgtatc gagctaggca cttaaataca atatctctgc aatgcggcaa
     2041 ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc cttcctaata aggcacgatc
     2101 gtaccacctt acttccacca atcggcatgc acggtgcttt ttctctcctt gtaaggcatg
     2161 ttgctaactc atcgttacca tgttgcaaga ctacaagagt attgcataag actacattaa
     2221 gcttgcagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     2281 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaaaggcct
     4381 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     4441 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     4501 aggggcggga tgggcggagt taggggcggg atagctagag ccagacatga taagatacat
     4561 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     4621 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttcctctcac
     4681 tctctgatat tcatttcttt gcaagttata aatactgaat aataagatga catgaactac
     4741 tactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     4801 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     4861 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     4921 acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc
     4981 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     5041 aaggtacctc tagtccggac gccgaggcga aacaggcggg gaggcggccc aaagggagat
     5101 ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc
     5161 gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg
     5221 cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct tccccgacaa
     5281 caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc
     5341 ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg
     5401 acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac
     5461 gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata
     5521 caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc
     5581 agcgtatcca catagcgtaa aaggagcaac atagttaaga atacgatatc ttggatcgat
     5641 cctcaatgca ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     5701 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     5761 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     5821 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     5881 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     5941 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     6001 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     6061 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     6121 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     6181 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     6241 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     6301 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     6361 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     6421 gcgtgcaatc catcttgttc aatcattcta gacattggac cagggttttc ttcaacatca
     6481 ccacaagtga ggagagaacc tctaccttct ccgcttcctt cgaacccggg gagcatgtca
     6541 aggtcaaaat cgtcaagagc gtcagcaggc agcatatcaa ggtcaaagtc gtcaagggca
     6601 tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt cggtcggccc gccgctttcg
     6661 cactttagct gtttctccag gccacatatg attagttcca ggccgaaaag gaaggcaggt
     6721 tcggctccct gccggtcgaa cagctcaatt gcttgtctca gaagtggggg catagaatcg
     6781 gtggtaggtg tctctctttc ctcttttgct acttgatgct cctgttcctc caatacgcag
     6841 cccagtgtaa agtggcccac ggcggacaga gcgtacagtg cgttctccag ggagaagcct
     6901 tgctgacaca ggaacgcgag ctgattttcc agggtttcgt actgtttctc tgttgggcgg
     6961 gtgccgagat gcactttagc cccgtcgcga tgtgagagga gagcacagcg gtatgacttg
     7021 gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca gggggcagaa gtgggtatga
     7081 tgcctgtcca gcatctcgat tggcagggca tcgagcaggg cccgcttgtt cttcacgtgc
     7141 cagtacaggg taggctgctc aactcccagc ttttgagcga gtttccttgt cgtcaggcct
     7201 tcgataccga caccattgag taattccaga gctccgttta tgactttgct cttgtccagt
     7261 ctagacatgg tggccgcgcg cgggtgaatt gctggggaga gaggtcggtg attcggtcaa
     7321 cgagggagcc gactgccgac gtgcgctccg gaggcttgca gaatgcggaa caccgcgcgg
     7381 gcaggaacag ggcccacact accgccccac accccgcctc ccgcaccgcc ccttcccggc
     7441 cgctgctctc ggcgcgccct gctgagcagc cgctattggc cacagcccat cgcggtcggc
     7501 gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt actagtgaga cgtgcggctt
     7561 ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc ttcccgactt aggggcggag
     7621 caggaagcgt cgccgggggg cccacaaggg tagcggcgaa gatccgggtg acgctgcgaa
     7681 cggacgtgaa gaatgtgcga gacccagggt cggcgccgct gcgtttcccg gaaccacgcc
     7741 cagagcagcc gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc aaccccaacc
     7801 ccggatcctg tacaacgcgt ctgcagccta ggaccggggt tttcttccac gtctcctgct
     7861 tgctttaaca gagagaagtt cgtggctccg gaaccggttt cttcaccggc atctgcatcc
     7921 ggggtcttga aggcgtgctg gtactccacg atgcccagct cggtgttgct gtgatcctcc
     7981 tccacgcggc ggaaggcgaa catggggccc ccgttctgca ggatgctggg gtggatggcg
     8041 ctcttgaagt gcatgtggct gtccaccacg gagctgtagt agccgccgtc gcgcaggctg
     8101 aaggtgcggg tgaagctgcc atccagatcg ttatcgccca tggggtgcag gtgctccacg
     8161 gtggcgttgc tgcggatgat cttgtcggtg aagatcacgc tgtcctcggg gaagccggtg
     8221 cccatcacct tgaagtcgcc gatcacgcgg ccggcctcgt agcggtagct gaagctcacg
     8281 tgcagcacgc cgccgtcctc gtacttctcg atgcgggtgt tggtgtagcc gccgttgttg
     8341 atggcgtgca ggaaggggtt ctcgtagccg ctggggtagg tgccgaagtg gtagaagccg
     8401 tagcccatca cgtggctcag caggtagggg ctgaaggtca gggcgccttt ggtgctcttc
     8461 atcttgttgg tcatgcggcc ctgctcgggg gtgccctctc cgccgcccac cagctcgaac
     8521 tccacgccgt tcagggtgcc ggtgatgcgg cactcgatct ccatggcggg caggccgctc
     8581 tcgtcgctct ccatggtggc gaattcgcta gccaattctc caggcgatct gacggttca
//
