LOCUS       sequence-176295-       13813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MESA target chain with V1-MESA ectodomain, 45 extracellular linkers,
            a flag tag, M cleavage sequence, and dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 13813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253."
COMMENT     Sequence Label: V1-MESA-45F-M-dCas9
FEATURES             Location/Qualifiers
     source          1..13813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             108..155
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(156..179,180..182,183..2738,2739..2741,2742..4256)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4311..4331
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4356..4505
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4527..4580
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4581..4979
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5004..5592
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5057..5077)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5475..5486)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5595..5611)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5596..5612)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6117..6297
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6323..6340)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6329..6553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6599..7027
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6686..6705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6896..6917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7036..7056)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7041..7370
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7221..7356
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7283..7302
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7418..7465
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7484..7858
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7988..8109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8025..8044)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8079..8098
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8158..8174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8158..8174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8171..8193)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8182..8198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8206..8212,8213..8230,8231..8236))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8251..8272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8389..8406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8560..9148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8640..8659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9319..10110,10111..10179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9942..9961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10180..10284)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10359..10378)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10550..10929
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10931..11129
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11084..11104
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11147..11327
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11374..11499
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11992..12225
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12410..12454
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12752..12869
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaccggtct ggtcgtggtt gctggagtcc tgttttgtta tggcttgcta gtgacagtgg
       61 ctctttgtgt tgaaaacctg tattttcaga tgcgtacggc caccatgaaa aggccggcgg
      121 ccacgaaaaa ggccggccag gcaaaaaaga aaaaggacaa gaagtacagc atcggcctgg
      181 ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
      241 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
      301 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
      361 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
      421 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
      481 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
      541 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
      601 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
      661 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
      721 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
      781 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
      841 ccggcgagaa gaagaatggc ctgttcggca acctgattgc cctgagcctg ggcctgaccc
      901 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
      961 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
     1021 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
     1081 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
     1141 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
     1201 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
     1261 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
     1321 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
     1381 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
     1441 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
     1501 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
     1561 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
     1621 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
     1681 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
     1741 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
     1801 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
     1861 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
     1921 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
     1981 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
     2041 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
     2101 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
     2161 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
     2221 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
     2281 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
     2341 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
     2401 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
     2461 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
     2521 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
     2581 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
     2641 tcaaccggct gtccgactac gatgtggacc acatcgtgcc tcagagcttt ctgaaggacg
     2701 actccatcga caacaaggtg ctgaccagaa gcgacaaggc ccggggcaag agcgacaacg
     2761 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
     2821 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
     2881 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
     2941 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
     3001 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
     3061 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
     3121 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt
     3181 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
     3241 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
     3301 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg
     3361 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
     3421 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
     3481 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
     3541 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
     3601 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
     3661 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag
     3721 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc
     3781 ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct
     3841 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg
     3901 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
     3961 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
     4021 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca
     4081 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca
     4141 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc
     4201 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacagcg
     4261 ctggaggagg tggaagcgga ggaggaggaa gcggaggagg aggtagcgga cctaagaaaa
     4321 agaggaaggt ggcggccgct ggatccggac gggctgacgc attggacgat tttgatctgg
     4381 atatgctggg aagtgacgcc ctcgatgatt ttgaccttga catgcttggt tcggatgccc
     4441 ttgatgactt tgacctcgac atgctcggca gtgacgccct tgatgatttc gacctggaca
     4501 tgctgattaa ctgtacaggc agtggagagg gcagaggaag tctgctaaca tgcggtgacg
     4561 tcgaggagaa tcctggccca atggccaagc ctttgtctca agaagaatcc accctcattg
     4621 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     4681 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     4741 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     4801 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     4861 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     4921 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     4981 aattcgatat caagcttatc ggtaatcaac ctctggatta caaaatttgt gaaagattga
     5041 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5101 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5161 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5221 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5281 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5341 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     5401 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5461 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5521 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5581 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     5641 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     5701 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     5761 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5821 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     5881 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     5941 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     6001 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     6061 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     6121 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6181 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6241 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     6301 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6361 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6421 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6481 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6541 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6601 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6661 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6721 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6781 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6841 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6901 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6961 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7021 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7081 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7141 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7201 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7261 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7321 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7381 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     7441 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     7501 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     7561 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     7621 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     7681 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     7741 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     7801 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     7861 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7921 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7981 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8041 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8101 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8161 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8221 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8281 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8341 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8401 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8461 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8521 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8581 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8641 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8701 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8761 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8821 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8881 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8941 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9001 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9061 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9121 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9301 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9361 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9421 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9481 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9541 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9601 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9661 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9721 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9781 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9841 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9901 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9961 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10021 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10081 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10141 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10201 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10261 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10321 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    10381 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    10441 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    10501 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
    10561 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    10621 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    10681 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    10741 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    10801 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    10861 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    10921 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    10981 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
    11041 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    11101 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
    11161 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    11221 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    11281 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
    11341 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
    11401 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    11461 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    11521 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    11581 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    11641 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    11701 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    11761 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    11821 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    11881 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    11941 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    12001 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    12061 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    12121 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    12181 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    12241 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    12301 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    12361 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    12421 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    12481 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    12541 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    12601 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    12661 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
    12721 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    12781 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    12841 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
    12901 tttggttaat tagctagcgc catcatggct tggaccagct taattcttag cttgcttgcc
    12961 ctgtgcagcg gtgcatcatc tgacatacag atgactcagt cacctagcag tctgtctgct
    13021 tcagtcggtg atagggtaac tatcacctgt cgggcatctc aggatgtgtc cacggcagtt
    13081 gcctggtacc agcagaagcc cggcaaggcg cctaagttgc ttatctattc agcttcattt
    13141 ttgtatagtg gggtgcctag ccggtttagc ggaagtggtt ctggcacaga cttcactttg
    13201 acaatcagtt ccctccaacc ggaggatttc gctacctact actgtcagca ggggtacgga
    13261 aaccccttta cgtttggaca gggcactaaa gtggagatta agaggggagg cggaggctcc
    13321 ggtgggggag gctctggtgg tggaggtagc gaggttcagc tggtggaaag tggtggcgga
    13381 ctagtacagc caggcggctc cctgcggctg agttgcgccg cgagtggctt tactatatct
    13441 gactattgga tccattgggt caggcaagcc cctgggaaag gattggagtg ggttgctggt
    13501 attacccccg caggtgggta tacttattat gcagatagcg tgaaagggag gttcaccatc
    13561 tctgcagaca caagcaagaa cacggcctac ctacagatga actctctgag ggcagaggac
    13621 actgccgtct actattgcgc ccgatttgtg ttttttctac cctacgccat ggactattgg
    13681 ggacagggga cattggtagg cgccgggaca ggagggtctg ggggtggcac tggcggatct
    13741 ggaggagact acaaggacga tgacgacaac ggcggatcgg gggggtctgg tggtagtggc
    13801 ggttcggggg gag
//
