LOCUS       sequence-176307-        8878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of UMPS (Y37A, R155A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Cantor JR, Abu-Remaileh M, Kanarek N, Freinkman E, Gao X, Louissaint
            A Jr, Lewis CA, Sabatini DM
  TITLE     Physiologic Medium Rewires Cellular Metabolism and Reveals Uric Acid
            as an Endogenous Inhibitor of UMP Synthase.
  JOURNAL   Cell. 2017 Apr 6;169(2):258-272.e17. doi: 
            10.1016/j.cell.2017.03.023.
  PUBMED    28388410
REFERENCE   2  (bases 1 to 8878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-6- 6";
            volume: "169"; issue: "2"; pages: "258-272.e17"
COMMENT     Sequence Label: pLJC2-UMPS (Y37A, R155A)-3xFLAG
FEATURES             Location/Qualifiers
     source          1..8878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1718..1783
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1831..1948
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1997..2507
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2426..2444
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2529..3128
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2529..2548)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3025..3045
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3256..3489
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3561..3682
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3598..3617)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3652..3671
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3722..3857
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3877..3896)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3878..3896)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3906..3923)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3906..3922)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3915..3937)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4064..4519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4151..4170)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4361..4382
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4545..4649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4650..4718,4719..5510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4868..4887)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5681..6269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6170..6189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6423..6440
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6557..6578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6593..6598,6599..6616,6617..6623)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6631..6647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6636..6658
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6655..6671
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6655..6671
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6690..6710
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6692..6710
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6738..6964
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6965..7145
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7192..7317
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7810..8043
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8228..8272
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8570..8590
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tttaattaac gcgacaatgg cggtcgctcg tgcagctttg gggccattgg tgacgggtct
      301 gtacgacgtg caggctttca agtttgggga cttcgtgctg aagagcgggc tttcctcccc
      361 catcgccatc gatctgcggg gcatcgtgtc tcgaccgcgt cttctgagtc aggttgcaga
      421 tattttattc caaactgccc aaaatgcagg catcagtttt gacaccgtgt gtggagtgcc
      481 ttatacagct ttgccattgg ctacagttat ctgttcaacc aatcaaattc caatgcttat
      541 tagaaggaaa gaaacaaagg attatggaac taagcgtctt gtagaaggaa ctattaatcc
      601 aggagaaacc tgtttaatca ttgaagatgt tgtcaccagt ggatctagtg ttttggaaac
      661 tgttgaggtt cttcagaagg agggcttgaa ggtcactgat gccatagtgc tgttggacgc
      721 cgagcaggga ggcaaggaca agttgcaggc gcacgggatc cgcctccact cagtgtgtac
      781 attgtccaaa atgctggaga ttctcgagca gcagaaaaaa gttgatgctg agacagttgg
      841 gagagtgaag aggtttattc aggagaatgt ctttgtggca gcgaatcata atggttctcc
      901 cctttctata aaggaagcac ccaaagaact cagcttcggt gcacgtgcag agctgcccag
      961 gatccaccca gttgcatcga agcttctcag gcttatgcaa aagaaggaga ccaatctgtg
     1021 tctatctgct gatgtttcac tggccagaga gctgttgcag ctagcagatg ctttaggacc
     1081 tagtatctgc atgctgaaga ctcatgtaga tattttgaat gattttactc tggatgtgat
     1141 gaaggagttg ataactctgg caaaatgcca tgagttcttg atatttgaag accggaagtt
     1201 tgcagatata ggaaacacag tgaaaaagca gtatgaagga ggtatcttta aaatagcttc
     1261 ctgggcagat ctagtaaatg ctcacgtggt gccaggctca ggagttgtga aaggcctgca
     1321 agaagtgggc ctgcctttgc atcgggggtg cctccttatt gcggaaatga gctccaccgg
     1381 ctccctggcc actggggact acactagagc agcggttaga atggctgagg agcactctga
     1441 atttgttgtt ggttttattt ctggctcccg agtaagcatg aaaccagaat ttcttcactt
     1501 gactccagga gttcagttgg aagcaggagg agataatctt ggccaacagt acaatagccc
     1561 acaagaagtt attggcaaac gaggttccga tatcatcatt gtaggtcgtg gcataatctc
     1621 agcagctgat cgtctggaag cagcagagat gtacagaaaa gctgcttggg aagcgtattt
     1681 gagtagactt ggtgttggag gcagcgcggc cgcaggcgac tacaaagacc atgacggtga
     1741 ttataaagat catgacattg attacaagga tgacgatgac aagtaataag aattctcgac
     1801 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     1861 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     1921 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     1981 ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     2041 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     2101 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     2161 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     2221 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     2281 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     2341 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     2401 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     2461 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccaccgg
     2521 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     2581 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     2641 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     2701 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     2761 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     2821 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     2881 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     2941 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     3001 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     3061 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     3121 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     3181 catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3241 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3301 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3481 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     3541 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     3601 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3661 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     3721 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3781 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3841 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     3901 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     3961 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4021 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     4081 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4141 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4201 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4261 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4321 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4381 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4441 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4501 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4561 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4621 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4681 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4741 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4801 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4861 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4921 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4981 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5041 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5101 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5161 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5221 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5281 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5341 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5401 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5461 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5521 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5581 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5641 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5701 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5761 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5821 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5881 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5941 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6001 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6061 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6421 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6481 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6541 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6601 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6661 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     6721 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     6781 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     6841 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     6901 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     6961 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7021 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7081 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7141 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     7201 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     7261 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     7321 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     7381 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     7441 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     7501 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     7561 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     7621 gagcaaaaca aaagtaagac caccgcacag caagcagcag ctgatcttca gacctggagg
     7681 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7741 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     7801 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     7861 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     7921 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     7981 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     8041 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     8101 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     8161 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8221 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8281 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     8341 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     8401 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     8461 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     8521 tcgacggtat cgatcacgag actagcctcg agcagcagcc cccttcaccg agggcctatt
     8581 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
     8641 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     8701 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgccaagtac
     8761 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     8821 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatg
//
