LOCUS       sequence-176331-        9731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4889
FEATURES             Location/Qualifiers
     source          1..9731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4598..4645
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4652..4732
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4806..4823)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4812..5036
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5082..5510
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5169..5188)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5379..5400
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5519..5539)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5524..5853
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5704..5839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5766..5785
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5920..6714
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5974..5993)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6584..6603
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6888..7009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6925..6944)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6979..6998
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7058..7074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7058..7074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7071..7093)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7082..7098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7106..7112,7113..7130,7131..7136))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7151..7172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7289..7306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7460..8048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7540..7559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8219..9010,9011..9079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8842..8861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9080..9184)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9259..9278)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(9434..9682)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(9492..9511)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(9662..9682)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
      601 gagaacccac tgcttactgg cttatcgaaa ttgatacgac tcactatagg gagacccaag
      661 ctggctagcg tttaaactta agcttggtac cgccaccatg agcatctacc aggagttcgt
      721 caacaagtat tcactgagta agacactgcg gttcgagctg atcccacagg gcaagacact
      781 ggagaacatc aaggcccgag gcctgattct ggacgatgag aagcgggcaa aagactataa
      841 gaaagccaag cagatcattg ataaatacca ccagttcttt atcgaggaaa ttctgagctc
      901 cgtgtgcatc agtgaggatc tgctgcagaa ttactcagac gtgtacttca agctgaagaa
      961 gagcgacgat gacaacctgc agaaggactt caagtccgcc aaggacacca tcaagaaaca
     1021 gattagcgag tacatcaagg actccgaaaa gtttaaaaat ctgttcaacc agaatctgat
     1081 cgatgctaag aaaggccagg agtccgacct gatcctgtgg ctgaaacagt ctaaggacaa
     1141 tgggattgaa ctgttcaagg ctaactccga tatcactgat attgacgagg cactggaaat
     1201 catcaagagc ttcaagggat ggaccacata ctttaaaggc ttccacgaga accgcaagaa
     1261 cgtgtactcc agcaacgaca ttcctacctc catcatctac cgaatcgtcg atgacaatct
     1321 gccaaagttc ctggagaaca aggccaaata tgaatctctg aaggacaaag ctcccgaggc
     1381 aattaattac gaacagatca agaaagatct ggctgaggaa ctgacattcg atatcgacta
     1441 taagactagc gaggtgaacc agagggtctt ttccctggac gaggtgtttg aaatcgccaa
     1501 tttcaacaat tacctgaacc agtccggcat tactaaattc aataccatca ttggcgggaa
     1561 gtttgtgaac ggggagaata ccaagcgcaa gggaattaac gaatacatca atctgtatag
     1621 ccagcagatc aacgacaaaa ctctgaagaa atacaagatg tctgtgctgt tcaaacagat
     1681 cctgagtgat accgagtcca agtcttttgt cattgataaa ctggaagatg actcagacgt
     1741 ggtcactacc atgcagagct tttatgagca gatcgccgct ttcaagacag tggaggaaaa
     1801 atctattaag gaaactctga gtctgctgtt cgatgacctg aaagcccaga agctggacct
     1861 gagtaagatc tacttcaaaa acgataagag tctgacagac ctgtcacagc aggtgtttga
     1921 tgactattcc gtgattggga ccgccgtcct ggagtacatt acacagcaga tcgctccaaa
     1981 gaacctggat aatccctcta agaaagagca ggaactgatc gctaagaaaa ccgagaaggc
     2041 aaaatatctg agtctggaaa caattaagct ggcactggag gagttcaaca agcacaggga
     2101 tattgacaaa cagtgccgct ttgaggaaat cctggccaac ttcgcagcca tccccatgat
     2161 ttttgatgag atcgcccaga acaaagacaa tctggctcag atcagtatta agtaccagaa
     2221 ccagggcaag aaagacctgc tgcaggcttc agcagaagat gacgtgaaag ccatcaagga
     2281 tctgctggac cagaccaaca atctgctgca caagctgaaa atcttccata ttagtcagtc
     2341 agaggataag gctaatatcc tggataaaga cgaacacttc tacctggtgt tcgaggaatg
     2401 ttacttcgag ctggcaaaca ttgtccccct gtataacaag attaggaact acatcacaca
     2461 gaagccttac tctgacgaga agtttaaact gaacttcgaa aatagtaccc tggccaacgg
     2521 gtgggataag aacaaggagc ctgacaacac agctatcctg ttcatcaagg atgacaagta
     2581 ctatctggga gtgatgaata agaaaaacaa taagatcttc gatgacaaag ccattaagga
     2641 gaacaaaggg gaaggataca agaaaatcgt gtataagctg ctgcccggcg caaataagat
     2701 gctgcctaag gtgttcttca gcgccaagag tatcaaattc tacaacccat ccgaggacat
     2761 cctgcggatt agaaatcact caacacatac taagaacggg agcccccaga agggatatga
     2821 gaaatttgag ttcaacatcg aggattgcag gaagtttatt gacttctaca agcagagcat
     2881 ctccaaacac cctgaatgga aggattttgg cttccggttt tccgacacac agagatataa
     2941 ctctatcgac gagttctacc gcgaggtgga aaatcagggg tataagctga cttttgagaa
     3001 catttctgaa agttacatcg acagcgtggt caatcaggga aagctgtacc tgttccagat
     3061 ctataacaaa gatttttcag catacagcaa gggcagacca aacctgcata cactgtactg
     3121 gaaggccctg ttcgatgaga ggaatctgca ggacgtggtc tataaactga acggagaggc
     3181 cgaactgttt taccggaagc agtctattcc taagaaaatc actcacccag ctaaggaggc
     3241 catcgctaac aagaacaagg acaatcctaa gaaagagagc gtgttcgaat acgatctgat
     3301 taaggacaag cggttcaccg aagataagtt ctttttccat tgtccaatca ccattaactt
     3361 caagtcaagc ggcgctaaca agttcaacga cgagatcaat ctgctgctga aggaaaaagc
     3421 aaacgatgtg cacatcctga gcattgctcg aggagagcgg catctggcct actataccct
     3481 ggtggatggc aaagggaata tcattaagca ggatacattc aacatcattg gcaatgaccg
     3541 gatgaaaacc aactaccacg ataaactggc tgcaatcgag aaggatagag actcagctag
     3601 gaaggactgg aagaaaatca acaacattaa ggagatgaag gaaggctatc tgagccaggt
     3661 ggtccatgag attgcaaagc tggtcatcga atacaatgcc attgtggtgt tcgaggatct
     3721 gaacttcggc tttaagaggg ggcgctttaa ggtggaaaaa caggtctatc agaagctgga
     3781 gaaaatgctg atcgaaaagc tgaattacct ggtgtttaaa gataacgagt tcgacaagac
     3841 cggaggcgtc ctgagagcct accagctgac agctcccttt gaaactttca agaaaatggg
     3901 aaaacagaca ggcatcatct actatgtgcc agccggattc acttccaaga tctgccccgt
     3961 gaccggcttt gtcaaccagc tgtaccctaa atatgagtca gtgagcaagt cccaggaatt
     4021 tttcagcaag ttcgataaga tctgttataa tctggacaag gggtacttcg agttttcctt
     4081 cgattacaag aacttcggcg acaaggccgc taaggggaaa tggaccattg cctccttcgg
     4141 atctcgcctg atcaactttc gaaattccga taaaaaccac aattgggaca ctagggaggt
     4201 gtacccaacc aaggagctgg aaaagctgct gaaagactac tctatcgagt atggacatgg
     4261 cgaatgcatc aaggcagcca tctgtggcga gagtgataag aaatttttcg ccaagctgac
     4321 ctcagtgctg aatacaatcc tgcagatgcg gaactcaaag accgggacag aactggacta
     4381 tctgattagc cccgtggctg atgtcaacgg aaacttcttc gacagcagac aggcacccaa
     4441 aaatatgcct caggatgcag acgccaacgg ggcctaccac atcgggctga agggactgat
     4501 gctgctgggc cggatcaaga acaatcagga ggggaagaag ctgaacctgg tcattaagaa
     4561 cgaggaatac ttcgagtttg tccagaatag aaataacaaa aggccggcgg ccacgaaaaa
     4621 ggccggccag gcaaaaaaga aaaagggatc ctacccatac gatgttccag attacgctta
     4681 tccctacgac gtgcctgatt atgcataccc atatgatgtc cccgactatg cctaagaatt
     4741 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg
     4801 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4861 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4921 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4981 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5041 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5101 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5161 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5221 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5281 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5341 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5401 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5461 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5521 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcagacag aagtatgcaa
     5581 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5641 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5701 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5761 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5821 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5881 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5941 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     6001 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     6061 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     6121 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6181 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6241 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6301 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     6361 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     6421 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     6481 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     6541 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     6601 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     6661 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     6721 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6781 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6841 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6901 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6961 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7021 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7081 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7141 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7201 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7261 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7321 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7381 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7441 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7501 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7561 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7621 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7681 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7741 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7801 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7861 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7921 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7981 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8041 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8101 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8161 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8221 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8281 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8341 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8401 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8461 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8521 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8581 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8641 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8701 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8761 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8821 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8881 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8941 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9001 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9061 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9121 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9181 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     9241 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     9301 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     9361 gcaaggcttg accgacaatt gcgtacaaaa aaggagacgt tcgaacgtct ccatctacaa
     9421 cagtagaaat tacggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat
     9481 actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa
     9541 actacccaag aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta
     9601 cagtcaaatt aattctaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag
     9661 gaatcatggg aaataggccc tcttcctgcc cgaccttaga tccgaatcca tatcgatatg
     9721 ctagttggcg c
//
