LOCUS       sequence-176453-        3822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab36 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(128..716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(208..227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(887..1678,1679..1747))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1510..1529
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1748..1852)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1930..2385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2067..2088)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2279..2298
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2511..2533
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2525..2542
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2526..2542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2549..2568
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2549..2567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2598..2607
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2602..2611
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3512..3530)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3513..3530)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3548..3564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3548..3564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3561..3583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3572..3588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3596..3602,3603..3620,3621..3626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3641..3662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3779..3796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
       61 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      121 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      181 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      241 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      301 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      361 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      421 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      481 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      541 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      601 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      661 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      721 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      781 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      841 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      901 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      961 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1021 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1081 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1141 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1201 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1261 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1321 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1381 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1441 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1501 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1561 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1621 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1681 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1741 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1861 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     1921 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     1981 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     2041 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     2101 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     2161 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     2221 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     2281 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     2341 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     2401 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     2461 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     2521 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattgggccc
     2581 gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgattcaat gcagttcagt
     2641 ccacctgtca gcagagacag gatcatatcc gcatttccaa agtgttataa tccacaagct
     2701 tgcctgcaaa tgaaggatga ctggaatgta gaagccaagg tggcttgcca agatcggacg
     2761 actcgtctgc aagggtatga caggtttaag atgtcaaagg cagtagtggt tggagatctc
     2821 aatgtgggaa agacatgctt aataaacagg ttctgtaaag acgtgtttga gagagactat
     2881 aaggctacta taggtgttga ttttgagatc gagagatttg agatgtctgg gctgcctttt
     2941 tctcttcaaa tatgggacac cgctggtcaa gaaaagttta aatgcattgc atcagcatat
     3001 tacagaggag ctcaagttat tatcactgtc tttgacatgg cggatattaa gaccttggaa
     3061 aacacaaaac agtggttgtt agaggcggtg cgggaaaatg aaccagactc ctgttttgtt
     3121 ttcttggttg gcacaaaaag agacctcctg tctacagaag agtgccagag gaccgagagg
     3181 gacgccgtaa agattgcagc agaaatgaat gcagaattct ggtcagtgtc ctcaaaaaca
     3241 ggtgaaaacg tccaagagtt tttctttcgt gtggctgctc tggcttttga agatgctgtc
     3301 atgaaggacc tggagtctgg gaccaccagc agcactcaga tcggagatgg cagtattctc
     3361 aatgataaaa cactggaagt cacgcaggag aagccaaaga agaagagctg ttgctgatgc
     3421 tctatggttc aaatcactag tgaattcgcg gccgcctgca ggtcgaccat atgggagagc
     3481 tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt
     3541 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3601 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3661 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3721 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3781 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg ag
//
