LOCUS       sequence-176561-       11996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses catalytically-inactive GFP-cGAS after lentiviral 
            transduction. Can be used to monitor nuclear envelope rupture 
            events..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Raab M, Gentili M, de Belly H, Thiam HR, Vargas P, Jimenez AJ, 
            Lautenschlaeger F, Voituriez R, Lennon-Dumenil AM, Manel N, Piel M
  TITLE     ESCRT III repairs nuclear envelope ruptures during cell migration to
            limit DNA damage and cell death.
  JOURNAL   Science. 2016 Apr 15;352(6283):359-62. doi: 10.1126/science.aad7611.
            Epub 2016 Mar 24.
  PUBMED    27013426
REFERENCE   2  (bases 1 to 11996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad7611"; journalName: "Science"; date: 
            "2016-04-15- 15"; volume: "352"; issue: "6283"; pages: "359-62"
COMMENT     Sequence Label: pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A
FEATURES             Location/Qualifiers
     source          1..11996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3254..3557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3558..3761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3712..3732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3807..3816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3813..3815,3816..3818,3819..4529)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3813..3815,3816..3818,3819..4529)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3858..3879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4119..4138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4466..4487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4539..4562
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6400..6580
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8108..8127)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8341..8360)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8460..8482
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8520..8538)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8606..8710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8711..8779,8780..9571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8929..8948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9742..10330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10231..10250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10484..10501
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10571..10591)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10576..10905
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10756..10891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtatagtaat caattacggg gtcattagtt catagcccat
     3241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     3781 tcccgcgggc tagcggatct gtcgacgcca ccatggtgag caagggcgag gagctgttca
     3841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4021 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4261 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4381 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4501 tcactctcgg catggacgag ctgtacaaga ctagtatgga ctacaaagac gatgacgaca
     4561 agatgcagcc ttggcacgga aaggccatgc agagagcttc cgaggccgga gccactgccc
     4621 ccaaggcttc cgcacggaat gccaggggcg ccccgatgga tcccaacgag tctccggctg
     4681 cccccgaggc cgccctgcct aaggcgggaa agttcggccc cgccaggaag tcgggatccc
     4741 ggcagaaaaa gagcgccccg gacacccagg agaggccgcc cgtccgcgca actggggccc
     4801 gcgccaaaaa ggcccctcag cgcgcccagg acacgcagcc gtctgacgcc accagcgccc
     4861 ctggggcaga ggggctggag cctcctgcgg ctcgggagcc ggctctttcc agggctggtt
     4921 cttgccgcca gaggggcgcg cgctgctcca cgaagccaag acccccgccc gggccctggg
     4981 acgtgcccag ccccggcctg ccggtctcgg cccccattct cgtacggagg gatgcggcgc
     5041 ctggggcctc gaagctccgg gcggttttgg agaagttgaa gctcagccgc gatgatatct
     5101 ccacggcggc ggggatggtg aaaggggttg tggaccacct gctgctcaga ctgaagtgcg
     5161 actccgcgtt cagaggcgtc gggctgctga acaccgggag ctactatgag cacgtgaaga
     5221 tttctgcacc taatgcattt gctgtcatgt ttaaactgga agtccccaga attcaactag
     5281 aagaatattc caacactcgt gcatattact ttgtgaaatt taaaagaaat ccgaaagaaa
     5341 atcatctgag tcagttttta gaaggtgaaa tattatcagc ttctaagatg ctgtcaaagt
     5401 ttaggaaaat cattaaggaa gaaattaacg acattaaaga tacagatgtc atcatgaaga
     5461 ggaaaagagg agggagccct gctgtaacac ttcttattag tgaaaaaata tctgtggata
     5521 taaccctggc tttggaatca aaaagtagct ggcctgctag cacccaagaa ggcctgcgca
     5581 ttcaaaactg gctttcagca aaagttagga agcaactacg actaaagcca ttttaccttg
     5641 tacccaagca tgcaaaggaa ggaaatggtt tccaagaaga aacatggcgg ctatccttct
     5701 ctcacatcga aaaggaaatt ttgaacaatc atggaaaatc taaaacgtgc tgtgaaaaca
     5761 aagaagagaa atgttgcagg aaagattgtt taaaactaat gaaatacctt ttagaacagc
     5821 tgaaagaaag gtttaaagac aaaaaacatc tggataaatt ctcttcttat catgtgaaaa
     5881 ctgccttctt tcacgtatgt acccagaacc ctcaagacag tcagtgggac cgcaaagacc
     5941 tgggcctctg ctttgataac tgcgtgacat actttcttca gtgcctcagg acagaaaaac
     6001 ttgagaatta ttttattcct gaattcaatc tattctctag caacttaatt gacaaaagaa
     6061 gtaaagaatt tctgacaaag caaattgaat atgaaagaaa caatgagttt ccagtttttg
     6121 atgaattttg actcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     6181 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     6241 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6301 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aaaatcgtcg
     6361 agagatgctg catataagca gctgcttttt gcttgtactg ggtctctctg gttagaccag
     6421 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6481 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6541 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     6601 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     6661 tataatagat ttagcaggaa ttgaactagg agtggagcac acaggcaaag ctgcagaagt
     6721 acttggaaga agccaccaga gatactcacg attctgcaca tacctggcta atcccagatc
     6781 ctaaggatta cattaagttt actaacattt atataatgat ttatagttta aagtataaac
     6841 ttatctaatt tactattctg acagatatta attaatcctc aaatatcata agagatgatt
     6901 actattatcc ccatttaaca caagaggaaa ctgagaggga aagatgttga agtaattttc
     6961 ccacaattac agcatccgtt agttacgact ctatgatctt ctgacacaaa ttccatttac
     7021 tcctcaccct atgactcagt cgaatatatc aaagttatgg acattatgct aagtaacaaa
     7081 ttaccctttt atatagtaaa tactgagtag attgagagaa gaaattgttt gcaaacctga
     7141 atagcttcaa gaagaagaga agtgaggata agaataacag ttgtcattta acaagtttta
     7201 acaagtaact tggttagaaa gggattcaaa tgcataaagc aagggataaa tttttctggc
     7261 aacaagacta tacaatataa ccttaaatat gacttcaaat aattgttgga acttgataaa
     7321 actaattaaa tattattgaa gattatcaat attataaatg taatttactt ttaaaaaggg
     7381 aacatagaaa tgtgtatcat tagagtagaa aacaatcctt attatcacaa tttgtcaaaa
     7441 caagtttgtt attaacacaa gtagaatact gcattcaatt aagttgactg cagattttgt
     7501 gttttgttaa aattagaaag agataacaac aatttgaatt attgaaagta acatgtaaat
     7561 agttctacat acgttctttt gacatcttgt tcaatcattg atcgaagttc tttatcttgg
     7621 aagaatttgt tccaaagact ctgaaataag gaaaacaatc tattatatag tctcacacct
     7681 ttgttttact tttagtgatt tcaatttaat aatgtaaatg gttaaaattt attcttctct
     7741 gagatcattt cacattgcag atagaaaacc tgagactggg gtaattttta ttaaaatcta
     7801 atttaatctc agaaacacat ctttattcta acatcaattt ttccagtttg atattatcat
     7861 ataaagtcag ccttcctcat ctgcaggttc cacaacaaaa atccaaccaa ctgtggatca
     7921 aaaatattgg gaaaaaatta aaaatagcaa tacaacaata aaaaaataca aatcagaaaa
     7981 acagcacagt ataacaactt tatttagcat ttacaatcta ttaggtatta taagtaatct
     8041 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     8101 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     8161 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     8221 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     8281 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     8341 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     8401 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     8461 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     8521 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     8581 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8641 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8701 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8761 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8821 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8881 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8941 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9001 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9061 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9121 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9181 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9241 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9301 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9361 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9421 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9481 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9541 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9601 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9661 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9721 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9781 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9841 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9901 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9961 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10021 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10081 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10141 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10201 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10261 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10321 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10381 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10441 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10501 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10561 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
    10621 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
    10681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
    10741 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    10801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    10861 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
    10921 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
    10981 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
    11041 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
    11101 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
    11161 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
    11221 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
    11281 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
    11341 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
    11401 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
    11461 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
    11521 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
    11581 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
    11641 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
    11701 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
    11761 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
    11821 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
    11881 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
    11941 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtg
//
