LOCUS       sequence-176724-        3678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab39bb cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      275..284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      279..288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(318..337)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(319..337)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(344..361)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(344..360)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(353..375)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      501..956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(588..607)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     798..819
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1034..1138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1139..1207,1208..1999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1357..1376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2170..2758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2659..2678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2912..2929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3046..3067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3082..3087,3088..3105,3106..3112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3120..3136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3125..3147
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3144..3160
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3144..3160
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3178..3196
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3178..3195
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cccacagagt tacggtagta tgctcgagta atagacctga aacgttcctg gcctgcagtg
       61 tcccagatct gcagtttaat gcgttttccg ggttcaatct ccaccaggcg ggagaaaaaa
      121 tcgaccccga ctgtcgggtc cgacacctgc gcgaaacggc cttctgtaaa tcgcctaatt
      181 aaacacgact ttccaacagt tgagtcccca atcacaatca gccgaaactg gtaaagccaa
      241 atcgcctcca tgtctgttca atcgaattcc cgcggccgcc atggcggccg ggagcatgcg
      301 acgtcgggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
      361 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      421 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      481 gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      541 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      601 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      661 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      721 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
      781 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
      841 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
      901 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg
      961 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt
     1021 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1081 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1141 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1201 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1261 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1321 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1381 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1441 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1501 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1561 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1621 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1681 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1741 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1801 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1861 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1921 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1981 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2041 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2101 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2281 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2341 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2401 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2461 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2521 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2581 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2641 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2701 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2761 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2821 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2881 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2941 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3001 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     3061 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3121 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctatt
     3181 taggtgacac tatagaatac tcaagctatg catccaacgc gttgggagct ctcccatatg
     3241 gtcgacctgc aggcggccgc gaattcacta gtgatttgat tgcggattca acagaagcac
     3301 cggcgatcac cctttgtcac ttcttctgac gaatgcacca cgtttggtac gaacccgctc
     3361 ttcactccct cccagccttc ctggatgcta atctccccat tcttgaccag ctcaaatata
     3421 tcccgcgtta gctctgtaaa agccctctcc acgtttatgg cgtcacgtgc cgaagtctcc
     3481 acgtagcgca taccgtatgc ggctgcaagc ttctcggcct cttgctggct cacctggcgc
     3541 tgtggttcca gatcacattt gtgcccgact agcaggaaga cgatgctgtg gggctgcacg
     3601 tgactgcgcg cctcctctag ccactcgtga acgttctgga aagagcggcg gttggtgatg
     3661 tcgaagagca gcaggccg
//
