LOCUS       sequence-176966-       12273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12273)
  AUTHORS   Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, 
            Li Y, Wang R, Yang S
  TITLE     CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
  JOURNAL   Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
  PUBMED    28469274
REFERENCE   2  (bases 1 to 12273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-4"; volume: "8:15179. doi"; pages: " 
            10.1038/ncomms15179"
COMMENT     Sequence Label: pJYS1Ptac
FEATURES             Location/Qualifiers
     source          1..12273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3671..4465
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3725..3744)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4335..4354
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      complement(8504..8531)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(8623..8708)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     9848..9867
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9887..9964)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(10198..10203,10204..10219,10220..10226)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    10234..10250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10239..10261
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             complement(11256..12206)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      join(12254..12273,1..203)
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
       61 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
      121 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
      181 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
      241 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
      301 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
      361 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
      421 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
      481 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
      541 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
      601 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
      661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      721 aaaggatctt cacctagatc ctttacgctc cgggacgcca acaacaagac ccatcatagt
      781 ttgcccccgc gacattgacc ataaattcat cgcacaaaat atcgaacggg gtttatgccg
      841 cttttagtgg gtgcgaagaa tagtctgctc attacccgcg aacaccgccg cattcagatc
      901 acgcttagta gcgtccccat gagtaggcag aaccgcgtcc aagtccacat catccataac
      961 gatcatgcac ggggtggaat ccacacccag acttgccagc acctcattag cgacacgttg
     1021 cgcagcggcc acgtccttag ccttatccac gcaatcgaga acgtactgcc taaccgcgaa
     1081 atcagactga atcagtttcc aatcatcggg cttcaccaaa gcaacagcaa cgcgggttga
     1141 ttcgacccgt tccggtgctt ccagaccggc gagcttgtac agttcttctt ccatttcacg
     1201 acgtacatca gcgtctatgt aatcaatgcc caaagcacgc ttagccccac gtgaccagga
     1261 cgaacgcagg tttttagaac caacctcata ctcacgccac cgagccacca aaacagcgtc
     1321 catatcctcg ccggcgtcgc tttgatcggc caacatatcc aacatctgaa acggcgtgta
     1381 cgacccctta gacgcggttt tagtagcgga gccagtcagt tcctgagaca tgcccttagc
     1441 gaggtaggtt gccattttcg cagcgtctcc accccaggta gacacctgat caagtttgac
     1501 cccgtgctca cgcagtggcg cgtccatacc ggccttaacc acaccagcag accagcggga
     1561 aaacatggaa tcctcaaacg ccttgagttc atcgtcagac agtggacgat ccaagaacaa
     1621 cagcatgttg cggtgcaagt gccaaccgtt cgcccaagag tctgtgacct catagtcact
     1681 ataggtgtgc tccaccccgt accgtgcacg ttctttcttc cactgagatg ttttcaccat
     1741 cgaagagtac gcagtcttaa tacccgcttc aacctgcgca aatgactgtg agcggttgtg
     1801 tcgaacagtg cccacaaaca tcatgagcgc gccacccgcc gccaagtgat tcttagtagc
     1861 aatagccagc tcaatgcggc gttcgcccat gacttccaat tcagccagag atgaccccca
     1921 gcgagagtga gagttttgca gaccctcaaa ctgcgaagca ccgttagacg accaggacac
     1981 cgcaacagct tcgtccctgc gccacctatg gcaccccgcc agagccttac tattggtgat
     2041 cttgtacatg acgttttgcc tacgccacgc cctagcgcga gtgaccttag aaccctcatt
     2101 gacctgcggt tccttagagg tgttcacttc tatttcagtg ttacctagac ccgatgttgt
     2161 gcggggttgc gcagtgcgag tttgtgcggg tgttgtgccc gttgtcttag ctagtgctat
     2221 ggttgtcaat tgaaacccct tcgggttatg tggcccccgt gcatatgagt tggtagctcg
     2281 cacgggggtt tgtcttgtct agggactatt aatttttagt ggtgtttggt ggccgcctag
     2341 cttggctatg cgtgccagct tacccgtact caatgttaaa gatttgcatc gacatgggag
     2401 ggttacgtgt ccgataccta gggggggtat ccgcgactag gtgccccggt gctcactgtc
     2461 tgtaccggcg gggcaagccc cacaccccgc atggacaggg tggctccgcc ccctgcaccc
     2521 ccagcaatct gcatgtacat gttttacaca ttagcacgac atgactgcat gtgcatgcac
     2581 tgcatgcaga ctaggtaaat atgagtatgt acgactagta acaggagcac tgcacataat
     2641 gaatgagttg caggacaatg tttgctacgc atgcgcatga catatcgcag gaaagctact
     2701 agagtcttaa agcatggcaa ccaaggcaca gctagaacag caactacaag aagctcaaca
     2761 ggcactacag gcgcagcaag cgcaggcaca agccaccatc gaagcactag aagcgcaggc
     2821 aaaggctaag cccgtcgtgg tcaccgcacg cgttcctttg gcactacgtg aggacatgaa
     2881 gcgcgcaggc atgcagaacg gtgaaaacct ccaagagttc atgatcgccg cgtttaccga
     2941 gcggctagaa aagctcacca ccaccgacaa cgaggaaaac aatgtctaac ccactagttc
     3001 tctttgccca ccgtgacccg gtaaatgacg tgacgttcga gtgcattgag cacgccacct
     3061 acgacacact ttcacacgct aaagaccaga tcaccgccca aatgcaagcc ctagacgaag
     3121 aagccgccct actgccctaa tgggtgtttc atgggtgttt ccctagtgtt tcatggtgtt
     3181 ttcacctaag ctagggaatt gcgcgagaag tctcgcaaaa atcagcaacc cccggaacca
     3241 cacagttcac gggggttctt ctatgccaga aatcagaaag gggaaccagt gaacgacccc
     3301 gaatggctgg atgatcctcc ttgggccacg cccacccggc gatcatcgag gcggtcaccg
     3361 gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
     3421 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     3481 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     3541 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
     3601 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg
     3661 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     3721 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     3781 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     3841 gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     3901 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     3961 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4021 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4081 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4141 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     4201 gatgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4261 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4321 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4381 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4441 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcggaatc atgacctgag
     4501 ctgtttacaa ttaatcatcg tgtggtacca tgtgtggaat tggaaaggac ttgaacgatg
     4561 tccatctacc aagagtttgt gaataaatac tccctgtcca agaccctccg ttttgagctg
     4621 atcccccaag gcaagaccct cgaaaacatc aaggcacgcg gcctcatcct ggatgacgaa
     4681 aagcgcgcta aggattacaa gaaggcaaag cagatcatcg acaagtacca ccagttcttc
     4741 atcgaagaga tcctgtcctc cgtgtgcatc tccgaggacc tgctccagaa ctactccgat
     4801 gtctacttca agctcaagaa gtccgatgac gataacctgc agaaggactt caagtccgct
     4861 aaggatacca tcaagaagca gatctccgaa tacatcaagg attccgagaa gttcaagaac
     4921 ctcttcaacc agaacctgat cgacgcaaag aagggccagg aatccgatct catcctgtgg
     4981 ctcaagcagt ccaaggataa cggcatcgag ctcttcaagg ccaactccga catcaccgac
     5041 atcgatgaag ctctggagat catcaagtcc ttcaagggct ggaccaccta cttcaagggc
     5101 ttccacgaaa accgcaagaa cgtgtactcc tccaacgata tcccaacctc tatcatctac
     5161 cgcatcgtcg acgataacct gccaaagttc ctcgaaaaca aggcaaagta cgagtccctg
     5221 aaggataagg ccccagaagc tatcaactac gagcagatca agaaggacct ggccgaagag
     5281 ctcaccttcg acatcgatta caagacctct gaagtgaacc agcgcgtctt ctccctcgat
     5341 gaagtgttcg agatcgccaa cttcaacaac tacctgaacc agtccggcat caccaagttc
     5401 aacaccatca tcggcggcaa gttcgtcaac ggcgaaaaca ccaagcgcaa gggcatcaac
     5461 gagtacatca acctctactc ccagcagatc aacgataaga ccctgaagaa gtacaagatg
     5521 tccgtgctct tcaagcagat cctgtccgac accgaatcca agtccttcgt catcgacaag
     5581 ctggaggacg attccgatgt ggtcaccacc atgcagtcct tctacgaaca gatcgcagcc
     5641 ttcaagaccg tggaagagaa gtccatcaag gagaccctct ccctgctctt cgacgatctg
     5701 aaggctcaga agctggatct ctccaagatc tacttcaaga acgacaagtc cctgaccgat
     5761 ctctcccagc aggtcttcga cgattactcc gtgatcggca ccgcagtcct ggaatacatc
     5821 acccagcaga tcgccccaaa gaacctcgat aacccatcca agaaggaaca ggagctgatc
     5881 gccaagaaga ccgaaaaggc taagtacctg tccctcgaga ccatcaagct ggctctcgaa
     5941 gagttcaaca agcaccgcga catcgataag cagtgccgct tcgaagagat cctcgcaaac
     6001 ttcgctgcaa tcccaatgat cttcgacgaa atcgcacaga acaaggataa cctggcccag
     6061 atctccatca agtaccagaa ccagggcaag aaggatctgc tccaggcctc cgctgaggac
     6121 gatgtgaagg caatcaagga cctgctcgat cagaccaaca acctgctcca caagctgaag
     6181 atcttccaca tctcccagtc cgaagacaag gccaacatcc tcgacaagga tgagcacttc
     6241 tacctggtgt tcgaagagtg ctacttcgaa ctcgctaaca tcgtcccact gtacaacaag
     6301 atccgcaact acatcaccca gaagccatac tccgatgaaa agttcaagct caacttcgag
     6361 aactccaccc tggcaaacgg ctgggacaag aacaaggaac cagataacac cgccatcctc
     6421 ttcatcaagg acgataagta ctacctgggc gtgatgaaca agaagaacaa caagatcttc
     6481 gacgataagg ccatcaagga aaacaagggc gagggctaca agaagatcgt gtacaagctg
     6541 ctcccaggcg ctaacaagat gctcccaaag gtcttcttct ccgcaaagtc catcaagttc
     6601 tacaacccat ccgaagatat cctgcgcatc cgcaaccact ccacccacac caagaacggc
     6661 tccccacaga agggctacga aaagttcgag ttcaacatcg aagactgccg caagttcatc
     6721 gatttctaca agcagtccat ctccaagcac ccagagtgga aggacttcgg cttccgcttc
     6781 tccgataccc agcgctacaa ctccatcgat gaattctacc gcgaagtgga gaaccagggc
     6841 tacaagctga ccttcgaaaa catctccgag tcctacatcg attccgtggt caaccagggc
     6901 aagctgtacc tcttccagat ctacaacaag gacttctccg cttactccaa gggccgccca
     6961 aacctgcaca ccctctactg gaaggcactc ttcgacgaac gcaacctgca ggatgtggtc
     7021 tacaagctca acggcgaagc agagctgttc taccgcaagc agtccatccc aaagaagatc
     7081 acccacccag ccaaggaagc aatcgccaac aagaacaagg ataacccaaa gaaggaatcc
     7141 gtgttcgagt acgacctgat caaggataag cgcttcaccg aggacaagtt cttcttccac
     7201 tgcccaatca ccatcaactt caagtcctcc ggcgccaaca agttcaacga tgaaatcaac
     7261 ctgctcctga aggagaaggc taacgacgtg cacatcctgt ccatcgatcg cggcgaacgc
     7321 cacctcgcct actacaccct ggtcgacggc aagggcaaca tcatcaagca ggacaccttc
     7381 aacatcatcg gcaacgatcg catgaagacc aactaccacg acaagctggc cgctatcgag
     7441 aaggaccgcg attccgctcg caaggattgg aagaagatca acaacatcaa ggaaatgaag
     7501 gaaggctacc tctcccaggt ggtccacgaa atcgctaagc tggtgatcga gtacaacgca
     7561 atcgtggtct tcgaagacct gaacttcggc ttcaagcgcg gccgcttcaa ggtggagaag
     7621 caggtctacc agaagctgga aaagatgctc atcgagaagc tgaactacct cgtgttcaag
     7681 gacaacgaat tcgataagac cggcggcgtc ctccgtgcat accagctgac cgccccattc
     7741 gagaccttca agaagatggg caagcagacc ggcatcatct actacgtgcc agctggcttc
     7801 acctctaaga tctgcccagt gaccggcttc gtcaaccagc tctacccaaa gtacgaatcc
     7861 gtctccaagt cccaggagtt cttctccaag ttcgacaaga tctgctacaa cctggataag
     7921 ggctacttcg aattctcctt cgactacaag aacttcggcg ataaggcagc caagggcaag
     7981 tggaccatcg catccttcgg ctcccgcctc atcaacttcc gcaactccga caagaaccac
     8041 aactgggata cccgcgaagt gtacccaacc aaggaactgg agaagctcct gaaggattac
     8101 tccatcgaat acggccacgg cgagtgcatc aaggctgcaa tctgcggcga atccgacaag
     8161 aagttcttcg caaagctgac ctctgtgctc aacaccatcc tgcagatgcg caactccaag
     8221 accggcaccg agctggatta cctcatctcc ccagtggccg acgtcaacgg caacttcttc
     8281 gattcccgcc aggctccaaa gaacatgcca caggacgctg atgcaaacgg cgcctaccac
     8341 atcggtctga agggtctcat gctcctgggt cgcatcaaga acaaccagga aggcaagaag
     8401 ctgaatctcg tcattaagaa cgaagaatac tttgaatttg tccagaaccg caataactaa
     8461 gtcgacctgc aggcatgcaa gcttaagagt ttgtagaaac gcaaaaaggc catccgtcag
     8521 gatggccttc tgcttaattt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     8581 tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     8641 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     8701 ttttatttaa attctagaaa gcttatcgat gataagctgt caaacatggc ctgtcgcttg
     8761 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     8821 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgcgg ggagaggcgg
     8881 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     8941 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     9001 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
     9061 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
     9121 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     9181 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     9241 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     9301 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     9361 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     9421 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     9481 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     9541 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     9601 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     9661 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     9721 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     9781 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     9841 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     9901 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg
     9961 tgtcaacgta aatgcatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg atccgggctt
    10021 atcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg
    10081 gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg
    10141 ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg
    10201 acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag
    10261 gaaacagaat taaaagatat gaccatgatt acgccaagct tgcatgcctg caggtcgact
    10321 ctagaggatc catgactaag caaccaccaa tcgcaaaagc cgatctgcaa aaaactcagg
    10381 gaaaccgtgc accagcagca gttaaaaata gcgacgtgat tagttttatt aaccagccat
    10441 caatgaaaga gcaactggca gcagctcttc cacgccatat gacggctgaa cgtatgatcc
    10501 gtatcgccac cacagaaatt cgtaaagttc cggcgttagg aaactgtgac actatgagtt
    10561 ttgtcagtgc gatcgtacag tgttcacagc tcggacttga gccaggtagc gccctcggtc
    10621 atgcatattt actgcctttt ggtaataaaa acgaaaagag cggtaaaaag aacgttcagc
    10681 taatcattgg ctatcgcggc atgattgatc tggctcgccg ttctggtcaa atcgccagcc
    10741 tgtcagcccg tgttgtccgt gaaggtgacg agtttagctt cgaatttggc cttgatgaaa
    10801 agttaataca ccgcccggga gaaaacgaag atgccccggt tacccacgtc tatgctgtcg
    10861 caagactgaa agacggaggt actcagtttg aagttatgac gcgcaaacag attgagctgg
    10921 tgcgcagcct gagtaaagct ggtaataacg ggccgtgggt aactcactgg gaagaaatgg
    10981 caaagaaaac ggctattcgt cgcctgttca aatatttgcc cgtatcaatt gagatccagc
    11041 gtgcagtatc aatggatgaa aaggaaccac tgacaatcga tcctgcagat tcctctgtat
    11101 taaccgggga atacagtgta atcgataatt cagaggaata attcagcctg gcggtgtaat
    11161 gcaccgccaa cttgaaagtt aacaggtgaa cagttgttct acttttgttt gttagtcttg
    11221 atgcttcact gatagataca agagccataa gaacctcaga tccttccgta tttagccagt
    11281 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
    11341 tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
    11401 gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg
    11461 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
    11521 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
    11581 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
    11641 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
    11701 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
    11761 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
    11821 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
    11881 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
    11941 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
    12001 acaccataag cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac
    12061 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
    12121 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
    12181 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
    12241 taccaattga gatgggctag tcaatgataa tta
//
