LOCUS       sequence-177100-        9835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to make Fosmids that can be converted into Illumina sequencable 
            libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina.
  JOURNAL   Genome Res. 2012 Nov;22(11):2241-9. doi: 10.1101/gr.138925.112. Epub
            2012 Jul 16.
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.138925.112"; journalName: "Genome Res"; date: "2012-11";
            volume: "22"; issue: "11"; pages: "2241-9"
COMMENT     Sequence Label: pFosill-1
FEATURES             Location/Qualifiers
     source          1..9835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(128..145)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(286..945)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     860..879
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(946..1048)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1975..2194
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2285..3040
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    3043..3293
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3619..4794
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4794..5765
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5838..6311
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6571..6969
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     primer_bind     complement(7111..7130)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7230..7252
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7290..7308)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7376..7480
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7481..7549,7550..8341)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7699..7718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8512..9100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9001..9020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9254..9271
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9371..9769
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9787..9820)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taatacgact cactataggg agaagcttaa tgatacggmt ctyhtachmm wckmcgagct
       61 tccgatctcc acgtgcatgc tggatccagc atgcacgtgg agattatgcc gtcttctgct
      121 tgagatccta tagtgtcacc taaatcgtat gcggccgccc gggccgtcga ccaattctca
      181 tgtttgacag cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc
      241 gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg
      301 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
      361 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
      421 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
      481 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
      541 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
      601 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
      661 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
      721 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
      781 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
      841 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
      901 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
      961 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1021 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     1081 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     1141 aggtatttat tcgcgataag ctcatggagc ggcgtaaccg tcgcacagga aggacagaga
     1201 aagcgcggat ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg
     1261 ccgccgctgc tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc
     1321 attcctatgc gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga
     1381 tgggacataa gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg
     1441 ctgcccggca ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac
     1501 aattatcctg agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg
     1561 ctgtttttgt ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag
     1621 tcgggaaaat ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc
     1681 gtttatagga agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat
     1741 atgccttcag gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg
     1801 tcagcaatgg acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca
     1861 gccagtagtg ctcgccgcag tcgagcgaca gggcgaagcc ctcgagtgag cgaggaagca
     1921 ccagggaaca gcacttatat attctgctta cacacgatgc ctgaaaaaac ttcccttggg
     1981 gttatccact tatccacggg gatattttta taattatttt ttttatagtt tttagatctt
     2041 cttttttaga gcgccttgta ggcctttatc catgctggtt ctagagaagg tgttgtgaca
     2101 aattgccctt tcagtgtgac aaatcaccct caaatgacag tcctgtctgt gacaaattgc
     2161 ccttaaccct gtgacaaatt gccctcagaa gaagctgttt tttcacaaag ttatccctgc
     2221 ttattgactc ttttttattt agtgtgacaa tctaaaaact tgtcacactt cacatggatc
     2281 tgtcatggcg gaaacagcgg ttatcaatca caagaaacgt aaaaatagcc cgcgaatcgt
     2341 ccagtcaaac gacctcactg aggcggcata tagtctctcc cgggatcaaa aacgtatgct
     2401 gtatctgttc gttgaccaga tcagaaaatc tgatggcacc ctacaggaac atgacggtat
     2461 ctgcgagatc catgttgcta aatatgctga aatattcgga ttgacctctg cggaagccag
     2521 taaggatata cggcaggcat tgaagagttt cgcggggaag gaagtggttt tttatcgccc
     2581 tgaagaggat gccggcgatg aaaaaggcta tgaatctttt ccttggttta tcaaacgtgc
     2641 gcacagtcca tccagagggc tttacagtgt acatatcaac ccatatctca ttcccttctt
     2701 tatcgggtta cagaaccggt ttacgcagtt tcggcttagt gaaacaaaag aaatcaccaa
     2761 tccgtatgcc atgcgtttat acgaatccct gtgtcagtat cgtaagccgg atggctcagg
     2821 catcgtctct ctgaaaatcg actggatcat agagcgttac cagctgcctc aaagttacca
     2881 gcgtatgcct gacttccgcc gccgcttcct gcaggtctgt gttaatgaga tcaacagcag
     2941 aactccaatg cgcctctcat acattgagaa aaagaaaggc cgccagacga ctcatatcgt
     3001 attttccttc cgcgatatca cttccatgac gacaggatag tctgagggtt atctgtcaca
     3061 gatttgaggg tggttcgtca catttgttct gacctactga gggtaatttg tcacagtttt
     3121 gctgtttcct tcagcctgca tggattttct catacttttt gaactgtaat ttttaaggaa
     3181 gccaaatttg agggcagttt gtcacagttg atttccttct ctttcccttc gtcatgtgac
     3241 ctgatatcgg gggttagttc gtcatcattg atgagggttg attatcacag tttattactc
     3301 tgaattggct atccgcgtgt gtacctctac ctggagtttt tcccacggtg gatatttctt
     3361 cttgcgctga gcgtaagagc tatctgacag aacagttctt ctttgcttcc tcgccagttc
     3421 gctcgctatg ctcggttaca cggctgcggc gagcgctagt gataataagt gactgaggta
     3481 tgtgctcttc ttatctcctt ttgtagtgtt gctcttattt taaacaactt tgcggttttt
     3541 tgatgacttt gcgattttgt tgttgctttg cagtaaattg caagatttaa taaaaaaacg
     3601 caaagcaatg attaaaggat gttcagaatg aaactcatgg aaacacttaa ccagtgcata
     3661 aacgctggtc atgaaatgac gaaggctatc gccattgcac agtttaatga tgacagcccg
     3721 gaagcgagga aaataacccg gcgctggaga ataggtgaag cagcggattt agttggggtt
     3781 tcttctcagg ctatcagaga tgccgagaaa gcagggcgac taccgcaccc ggatatggaa
     3841 attcgaggac gggttgagca acgtgttggt tatacaattg aacaaattaa tcatatgcgt
     3901 gatgtgtttg gtacgcgatt gcgacgtgct gaagacgtat ttccaccggt gatcggggtt
     3961 gctgcccata aaggtggcgt ttacaaaacc tcagtttctg ttcatcttgc tcaggatctg
     4021 gctctgaagg ggctacgtgt tttgctcgtg gaaggtaacg acccccaggg aacagcctca
     4081 atgtatcacg gatgggtacc agatcttcat attcatgcag aagacactct cctgcctttc
     4141 tatcttgggg aaaaggacga tgtcacttat gcaataaagc ccacttgctg gccggggctt
     4201 gacattattc cttcctgtct ggctctgcac cgtattgaaa ctgagttaat gggcaaattt
     4261 gatgaaggta aactgcccac cgatccacac ctgatgctcc gactggccat tgaaactgtt
     4321 gctcatgact atgatgtcat agttattgac agcgcgccta acctgggtat cggcacgatt
     4381 aatgtcgtat gtgctgctga tgtgctgatt gttcccacgc ctgctgagtt gtttgactac
     4441 acctccgcac tgcagttttt cgatatgctt cgtgatctgc tcaagaacgt tgatcttaaa
     4501 gggttcgagc ctgatgtacg tattttgctt accaaataca gcaatagtaa tggctctcag
     4561 tccccgtgga tggaggagca aattcgggat gcctggggaa gcatggttct aaaaaatgtt
     4621 gtacgtgaaa cggatgaagt tggtaaaggt cagatccgga tgagaactgt ttttgaacag
     4681 gccattgatc aacgctcttc aactggtgcc tggagaaatg ctctttctat ttgggaacct
     4741 gtctgcaatg aaattttcga tcgtctgatt aaaccacgct gggagattag ataatgaagc
     4801 gtgcgcctgt tattccaaaa catacgctca atactcaacc ggttgaagat acttcgttat
     4861 cgacaccagc tgccccgatg gtggattcgt taattgcgcg cgtaggagta atggctcgcg
     4921 gtaatgccat tactttgcct gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc
     4981 ggggtgatag tgttgagaag acctctcggg tatggtcagg taatgaacgt gaccaggagc
     5041 tgcttactga ggacgcactg gatgatctca tcccttcttt tctactgact ggtcaacaga
     5101 caccggcgtt cggtcgaaga gtatctggtg tcatagaaat tgccgatggg agtcgccgtc
     5161 gtaaagctgc tgcacttacc gaaagtgatt atcgtgttct ggttggcgag ctggatgatg
     5221 agcagatggc tgcattatcc agattgggta acgattatcg cccaacaagt gcttatgaac
     5281 gtggtcagcg ttatgcaagc cgattgcaga atgaatttgc tggaaatatt tctgcgctgg
     5341 ctgatgcgga aaatatttca cgtaagatta ttacccgctg tatcaacacc gccaaattgc
     5401 ctaaatcagt tgttgctctt ttttctcacc ccggtgaact atctgcccgg tcaggtgatg
     5461 cacttcaaaa agcctttaca gataaagagg aattacttaa gcagcaggca tctaaccttc
     5521 atgagcagaa aaaagctggg gtgatatttg aagctgaaga agttatcact cttttaactt
     5581 ctgtgcttaa aacgtcatct gcatcaagaa ctagtttaag ctcacgacat cagtttgctc
     5641 ctggagcgac agtattgtat aagggcgata aaatggtgct taacctggac aggtctcgtg
     5701 ttccaactga gtgtatagag aaaattgagg ccattcttaa ggaacttgaa aagccagcac
     5761 cctgatgcga ccacgtttta gtctacgttt atctgtcttt acttaatgtc ctttgttaca
     5821 ggccagaaag cataactggc ctgaatattc tctctgggcc cactgttcca cttgtatcgt
     5881 cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg attattagtc
     5941 tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc acggtcccac
     6001 tcgtatcgtc ggtctgataa tcagactggg accacggtcc cactcgtatc gtcggtctga
     6061 ttattagtct gggaccatgg tcccactcgt atcgtcggtc tgattattag tctgggacca
     6121 cggtcccact cgtatcgtcg gtctgattat tagtctggaa ccacggtccc actcgtatcg
     6181 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt
     6241 ctgggaccac gatcccactc gtgttgtcgg tctgattatc ggtctgggac cacggtccca
     6301 cttgtattgt cgatcagact atcagcgtga gactacgatt ccatcaatgc ctgtcaaggg
     6361 caagtattga catgtcgtcg taacctgtag aacggagtaa cctcggtgtg cggttgtatg
     6421 cctgctgtgg attgctgctg tgtcctgctt atccacaaca ttttgcgcac ggttatgtgg
     6481 acaaaatacc tggttaccca ggccgtgccg gcacgttaac cgggctgcat ccgatgcaag
     6541 tgtgtcgctg tcgacgagct cgcgagctcg gacatgaggt tgccccgtat tcagtgtcgc
     6601 tgatttgtat tgtctgaagt tgtttttacg ttaagttgat gcagatcaat taatacgata
     6661 cctgcgtcat aattgattat ttgacgtggt ttgatggcct ccacgcacgt tgtgatatgt
     6721 agatgataat cattatcact ttacgggtcc tttccggtga tccgacaggt tacggggcgg
     6781 cgacctcgcg ggttttcgct atttatgaaa attttccggt ttaaggcgtt tccgttcttc
     6841 ttcgtcataa cttaatgttt ttatttaaaa taccctctga aaagaaagga aacgacaggt
     6901 gctgaaagcg agctttttgg cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga
     6961 acaatggaag tcctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7021 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7081 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7141 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7201 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7261 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7321 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7381 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7441 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7501 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7561 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7621 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7681 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7741 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7801 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7861 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7921 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7981 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8041 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8101 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8161 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8221 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8281 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8341 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8401 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8461 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8521 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8581 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8641 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8701 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8761 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8821 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8881 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8941 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9001 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9061 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9121 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9181 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9241 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9301 gcctctcccc gcgcgttggc cgattcatta atgcagcttg cgccgttcca cttcgatgcg
     9361 tcagtgaagc gacatgaggt tgccccgtat tcagtgtcgc tgatttgtat tgtctgaagt
     9421 tgtttttacg ttaagttgat gcagatcaat taatacgata cctgcgtcat aattgattat
     9481 ttgacgtggt ttgatggcct ccacgcacgt tgtgatatgt agatgataat cattattact
     9541 ttacgggtcc tttccggtga tccgacaggt tacggggcgg cgacctcgcg ggttttcgct
     9601 atttatgaaa attttccggt ttaaggcgtt tccgttcttc ttcgtcataa cttaatgttt
     9661 ttatttaaaa taccctctga aaagaaagga aacgacaggt gctgaaagcg agctttttgg
     9721 cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga acaatggaag tccgagctca
     9781 tcgctaataa cttcgtatag catacattat acgaagttat attcgatgcg gccgc
//
