LOCUS       sequence-177103-        9788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to make Fosmids that can be converted into Illumina sequencable 
            libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina.
  JOURNAL   Genome Res. 2012 Nov;22(11):2241-9. doi: 10.1101/gr.138925.112. Epub
            2012 Jul 16.
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.138925.112"; journalName: "Genome Res"; date: "2012-11";
            volume: "22"; issue: "11"; pages: "2241-9"
COMMENT     Sequence Label: pFosill-2
FEATURES             Location/Qualifiers
     source          1..9788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(21..38)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(179..838)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     753..772
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(839..941)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1868..2087
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2178..2933
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    2936..3186
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3512..4687
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4687..5658
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5731..6204
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6464..6862
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     primer_bind     complement(7004..7023)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7123..7145
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7183..7201)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7269..7373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7374..7442,7443..8234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7592..7611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8405..8993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8894..8913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9147..9164
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9264..9662
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9680..9713)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     9729..9748
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9729..9747
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gccgtcttct gcttgagatc ctatagtgtc acctaaatcg tatgcggccg cccgggccgt
       61 cgaccaattc tcatgtttga cagcttatca tcgaatttct gccattcatc cgcttattat
      121 cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct taaaaaaatt
      181 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
      241 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
      301 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
      361 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
      421 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
      481 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
      541 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
      601 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa
      661 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
      721 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
      781 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
      841 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
      901 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa
      961 agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt
     1021 gatcttccgt cacaggtatt tattcgcgat aagctcatgg agcggcgtaa ccgtcgcaca
     1081 ggaaggacag agaaagcgcg gatctgggaa gtgacggaca gaacggtcag gacctggatt
     1141 ggggaggcgg ttgccgccgc tgctgctgac ggtgtgacgt tctctgttcc ggtcacacca
     1201 catacgttcc gccattccta tgcgatgcac atgctgtatg ccggtatacc gctgaaagtt
     1261 ctgcaaagcc tgatgggaca taagtccatc agttcaacgg aagtctacac gaaggttttt
     1321 gcgctggatg tggctgcccg gcaccgggtg cagtttgcga tgccggagtc tgatgcggtt
     1381 gcgatgctga aacaattatc ctgagaataa atgccttggc ctttatatgg aaatgtggaa
     1441 ctgagtggat atgctgtttt tgtctgttaa acagagaagc tggctgttat ccactgagaa
     1501 gcgaacgaaa cagtcgggaa aatctcccat tatcgtagag atccgcatta ttaatctcag
     1561 gagcctgtgt agcgtttata ggaagtagtg ttctgtcatg atgcctgcaa gcggtaacga
     1621 aaacgatttg aatatgcctt caggaacaat agaaatcttc gtgcggtgtt acgttgaagt
     1681 ggagcggatt atgtcagcaa tggacagaac aacctaatga acacagaacc atgatgtggt
     1741 ctgtcctttt acagccagta gtgctcgccg cagtcgagcg acagggcgaa gccctcgagt
     1801 gagcgaggaa gcaccaggga acagcactta tatattctgc ttacacacga tgcctgaaaa
     1861 aacttccctt ggggttatcc acttatccac ggggatattt ttataattat tttttttata
     1921 gtttttagat cttctttttt agagcgcctt gtaggccttt atccatgctg gttctagaga
     1981 aggtgttgtg acaaattgcc ctttcagtgt gacaaatcac cctcaaatga cagtcctgtc
     2041 tgtgacaaat tgcccttaac cctgtgacaa attgccctca gaagaagctg ttttttcaca
     2101 aagttatccc tgcttattga ctctttttta tttagtgtga caatctaaaa acttgtcaca
     2161 cttcacatgg atctgtcatg gcggaaacag cggttatcaa tcacaagaaa cgtaaaaata
     2221 gcccgcgaat cgtccagtca aacgacctca ctgaggcggc atatagtctc tcccgggatc
     2281 aaaaacgtat gctgtatctg ttcgttgacc agatcagaaa atctgatggc accctacagg
     2341 aacatgacgg tatctgcgag atccatgttg ctaaatatgc tgaaatattc ggattgacct
     2401 ctgcggaagc cagtaaggat atacggcagg cattgaagag tttcgcgggg aaggaagtgg
     2461 ttttttatcg ccctgaagag gatgccggcg atgaaaaagg ctatgaatct tttccttggt
     2521 ttatcaaacg tgcgcacagt ccatccagag ggctttacag tgtacatatc aacccatatc
     2581 tcattccctt ctttatcggg ttacagaacc ggtttacgca gtttcggctt agtgaaacaa
     2641 aagaaatcac caatccgtat gccatgcgtt tatacgaatc cctgtgtcag tatcgtaagc
     2701 cggatggctc aggcatcgtc tctctgaaaa tcgactggat catagagcgt taccagctgc
     2761 ctcaaagtta ccagcgtatg cctgacttcc gccgccgctt cctgcaggtc tgtgttaatg
     2821 agatcaacag cagaactcca atgcgcctct catacattga gaaaaagaaa ggccgccaga
     2881 cgactcatat cgtattttcc ttccgcgata tcacttccat gacgacagga tagtctgagg
     2941 gttatctgtc acagatttga gggtggttcg tcacatttgt tctgacctac tgagggtaat
     3001 ttgtcacagt tttgctgttt ccttcagcct gcatggattt tctcatactt tttgaactgt
     3061 aatttttaag gaagccaaat ttgagggcag tttgtcacag ttgatttcct tctctttccc
     3121 ttcgtcatgt gacctgatat cgggggttag ttcgtcatca ttgatgaggg ttgattatca
     3181 cagtttatta ctctgaattg gctatccgcg tgtgtacctc tacctggagt ttttcccacg
     3241 gtggatattt cttcttgcgc tgagcgtaag agctatctga cagaacagtt cttctttgct
     3301 tcctcgccag ttcgctcgct atgctcggtt acacggctgc ggcgagcgct agtgataata
     3361 agtgactgag gtatgtgctc ttcttatctc cttttgtagt gttgctctta ttttaaacaa
     3421 ctttgcggtt ttttgatgac tttgcgattt tgttgttgct ttgcagtaaa ttgcaagatt
     3481 taataaaaaa acgcaaagca atgattaaag gatgttcaga atgaaactca tggaaacact
     3541 taaccagtgc ataaacgctg gtcatgaaat gacgaaggct atcgccattg cacagtttaa
     3601 tgatgacagc ccggaagcga ggaaaataac ccggcgctgg agaataggtg aagcagcgga
     3661 tttagttggg gtttcttctc aggctatcag agatgccgag aaagcagggc gactaccgca
     3721 cccggatatg gaaattcgag gacgggttga gcaacgtgtt ggttatacaa ttgaacaaat
     3781 taatcatatg cgtgatgtgt ttggtacgcg attgcgacgt gctgaagacg tatttccacc
     3841 ggtgatcggg gttgctgccc ataaaggtgg cgtttacaaa acctcagttt ctgttcatct
     3901 tgctcaggat ctggctctga aggggctacg tgttttgctc gtggaaggta acgaccccca
     3961 gggaacagcc tcaatgtatc acggatgggt accagatctt catattcatg cagaagacac
     4021 tctcctgcct ttctatcttg gggaaaagga cgatgtcact tatgcaataa agcccacttg
     4081 ctggccgggg cttgacatta ttccttcctg tctggctctg caccgtattg aaactgagtt
     4141 aatgggcaaa tttgatgaag gtaaactgcc caccgatcca cacctgatgc tccgactggc
     4201 cattgaaact gttgctcatg actatgatgt catagttatt gacagcgcgc ctaacctggg
     4261 tatcggcacg attaatgtcg tatgtgctgc tgatgtgctg attgttccca cgcctgctga
     4321 gttgtttgac tacacctccg cactgcagtt tttcgatatg cttcgtgatc tgctcaagaa
     4381 cgttgatctt aaagggttcg agcctgatgt acgtattttg cttaccaaat acagcaatag
     4441 taatggctct cagtccccgt ggatggagga gcaaattcgg gatgcctggg gaagcatggt
     4501 tctaaaaaat gttgtacgtg aaacggatga agttggtaaa ggtcagatcc ggatgagaac
     4561 tgtttttgaa caggccattg atcaacgctc ttcaactggt gcctggagaa atgctctttc
     4621 tatttgggaa cctgtctgca atgaaatttt cgatcgtctg attaaaccac gctgggagat
     4681 tagataatga agcgtgcgcc tgttattcca aaacatacgc tcaatactca accggttgaa
     4741 gatacttcgt tatcgacacc agctgccccg atggtggatt cgttaattgc gcgcgtagga
     4801 gtaatggctc gcggtaatgc cattactttg cctgtatgtg gtcgggatgt gaagtttact
     4861 cttgaagtgc tccggggtga tagtgttgag aagacctctc gggtatggtc aggtaatgaa
     4921 cgtgaccagg agctgcttac tgaggacgca ctggatgatc tcatcccttc ttttctactg
     4981 actggtcaac agacaccggc gttcggtcga agagtatctg gtgtcataga aattgccgat
     5041 gggagtcgcc gtcgtaaagc tgctgcactt accgaaagtg attatcgtgt tctggttggc
     5101 gagctggatg atgagcagat ggctgcatta tccagattgg gtaacgatta tcgcccaaca
     5161 agtgcttatg aacgtggtca gcgttatgca agccgattgc agaatgaatt tgctggaaat
     5221 atttctgcgc tggctgatgc ggaaaatatt tcacgtaaga ttattacccg ctgtatcaac
     5281 accgccaaat tgcctaaatc agttgttgct cttttttctc accccggtga actatctgcc
     5341 cggtcaggtg atgcacttca aaaagccttt acagataaag aggaattact taagcagcag
     5401 gcatctaacc ttcatgagca gaaaaaagct ggggtgatat ttgaagctga agaagttatc
     5461 actcttttaa cttctgtgct taaaacgtca tctgcatcaa gaactagttt aagctcacga
     5521 catcagtttg ctcctggagc gacagtattg tataagggcg ataaaatggt gcttaacctg
     5581 gacaggtctc gtgttccaac tgagtgtata gagaaaattg aggccattct taaggaactt
     5641 gaaaagccag caccctgatg cgaccacgtt ttagtctacg tttatctgtc tttacttaat
     5701 gtcctttgtt acaggccaga aagcataact ggcctgaata ttctctctgg gcccactgtt
     5761 ccacttgtat cgtcggtctg ataatcagac tgggaccacg gtcccactcg tatcgtcggt
     5821 ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg
     5881 accacggtcc cactcgtatc gtcggtctga taatcagact gggaccacgg tcccactcgt
     5941 atcgtcggtc tgattattag tctgggacca tggtcccact cgtatcgtcg gtctgattat
     6001 tagtctggga ccacggtccc actcgtatcg tcggtctgat tattagtctg gaaccacggt
     6061 cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg
     6121 tctgattatt agtctgggac cacgatccca ctcgtgttgt cggtctgatt atcggtctgg
     6181 gaccacggtc ccacttgtat tgtcgatcag actatcagcg tgagactacg attccatcaa
     6241 tgcctgtcaa gggcaagtat tgacatgtcg tcgtaacctg tagaacggag taacctcggt
     6301 gtgcggttgt atgcctgctg tggattgctg ctgtgtcctg cttatccaca acattttgcg
     6361 cacggttatg tggacaaaat acctggttac ccaggccgtg ccggcacgtt aaccgggctg
     6421 catccgatgc aagtgtgtcg ctgtcgacga gctcgcgagc tcggacatga ggttgccccg
     6481 tattcagtgt cgctgatttg tattgtctga agttgttttt acgttaagtt gatgcagatc
     6541 aattaatacg atacctgcgt cataattgat tatttgacgt ggtttgatgg cctccacgca
     6601 cgttgtgata tgtagatgat aatcattatc actttacggg tcctttccgg tgatccgaca
     6661 ggttacgggg cggcgacctc gcgggttttc gctatttatg aaaattttcc ggtttaaggc
     6721 gtttccgttc ttcttcgtca taacttaatg tttttattta aaataccctc tgaaaagaaa
     6781 ggaaacgaca ggtgctgaaa gcgagctttt tggcctctgt cgtttccttt ctctgttttt
     6841 gtccgtggaa tgaacaatgg aagtcctggc gtaatagcga agaggcccgc accgatcgcc
     6901 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     6961 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     7021 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7081 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7141 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7201 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7261 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7321 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7381 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7441 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7501 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7561 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7621 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7681 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7741 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7801 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7861 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7921 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7981 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8041 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8101 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8161 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8221 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8521 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8761 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8821 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8881 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8941 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9001 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9061 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9121 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9181 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc ttgcgccgtt
     9241 ccacttcgat gcgtcagtga agcgacatga ggttgccccg tattcagtgt cgctgatttg
     9301 tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt
     9361 cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata tgtagatgat
     9421 aatcattatt actttacggg tcctttccgg tgatccgaca ggttacgggg cggcgacctc
     9481 gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca
     9541 taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa
     9601 gcgagctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg
     9661 aagtccgagc tcatcgctaa taacttcgta tagcatacat tatacgaagt tatattcgat
     9721 gcggccgcta atacgactca ctatagggag aagcttaatg atacggcgmc gagcccgtgc
     9781 atgctgga
//
