LOCUS       sequence-177121-       12722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12722)
  AUTHORS   Chen LF, Mu Y, Greene WC
  TITLE     Acetylation of RelA at discrete sites regulates distinct nuclear 
            functions of NF-kappaB.
  JOURNAL   EMBO J. 2002 Dec 2. 21(23):6539-48.
  PUBMED    12456660
REFERENCE   2  (bases 1 to 12722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2002 Dec 2. 21(23):6539-48."
COMMENT     Sequence Label: pcDNA3.1-300(HAT-)
FEATURES             Location/Qualifiers
     source          1..12722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        233..612
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        613..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     767..787
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     861..880
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        861..879
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     932..948
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     932..948
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             4159..6009
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYAEILIGYLAAVKKSGATTGAIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      5773..5782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8257..8274
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8311..8328)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8317..8541
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8587..9015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8674..8693)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8884..8905
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9024..9044)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9029..9358
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9209..9344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9271..9290
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9425..10219
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9479..9498)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10089..10108
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10393..10514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10430..10449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10484..10503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10563..10579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10563..10579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10576..10598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10587..10603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10611..10617,10618..10635,10636..10641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10656..10677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10794..10811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10965..11553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11045..11064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11724..12515,12516..12584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12347..12366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12585..12689)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
       61 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
      121 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      181 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      241 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      301 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      361 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      421 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      481 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      541 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      601 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      661 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      721 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      781 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
      841 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt
      901 ttaaacgggc cctctagact cgagcggccg ctctagaact agtggatccc ccgggccgaa
      961 gaagagattt cctgaggatt ctggttttcc tcgcttgtat ctccgaaaga attaaaaatg
     1021 gccgagaatg tggtggaacc ggggccgcct tcagccaagc ggcctaaact ctcatctccg
     1081 gccctctcgg cgtccgccag cgatggcaca gattttggct ctctatttga cttggagcac
     1141 gacttaccag atgaattaat caactctaca gaattgggac taaccaatgg tggtgatatt
     1201 aatcagcttc agacaagtct tggcatggta caagatgcag cttctaaaca taaacagctg
     1261 tcagaattgc tgcgatctgg tagttcccct aacctcaata tgggagttgg tggcccaggt
     1321 caagtcatgg ccagccaggc ccaacagagc agtcctggat taggtttgat aaatagcatg
     1381 gtcaaaagcc caatgacaca ggcaggcttg acttctccca acatggggat gggcactagt
     1441 ggaccaaatc agggtcctac gcagtcaaca ggtatgatga acagtccagt aaatcagcct
     1501 gccatgggaa tgaacacagg gatgaatgcg ggcatgaatc ctggaatgtt ggctgcaggc
     1561 aatggacaag ggataatgcc taatcaagtc atgaacggtt caattggagc aggccgaggg
     1621 cgacagaata tgcagtaccc aaacccaggc atgggaagtg ctggcaactt actgactgag
     1681 cctcttcagc agggctctcc ccagatggga ggacaaacag gattgagagg cccccagcct
     1741 cttaagatgg gaatgatgaa caaccccaat ccttatggtt caccatatac tcagaatcct
     1801 ggacagcaga ttggagccag tggccttggt ctccagattc agacaaaaac tgtactatca
     1861 aataacttat ctccatttgc tatggacaaa aaggcagttc ctggtggagg aatgcccaac
     1921 atgggtcaac agccagcccc gcaggtccag cagccaggtc tggtgactcc agttgcccaa
     1981 gggatgggtt ctggagcaca tacagctgat ccagagaagc gcaagctcat ccagcagcag
     2041 cttgttctcc ttttgcatgc tcacaagtgc cagcgccggg aacaggccaa tggggaagtg
     2101 aggcagtgca accttcccca ctgtcgcaca atgaagaatg tcctaaacca catgacacac
     2161 tgccagtcag gcaagtcttg ccaagtggca cactgtgcat cttctcgaca aatcatttca
     2221 cactggaaga attgtacaag acatgattgt cctgtgtgtc tccccctcaa aaatgctggt
     2281 gataagagaa atcaacagcc aattttgact ggagcacccg ttggacttgg aaatcctagc
     2341 tctctagggg tgggtcaaca gtctgccccc aacctaagca ctgttagtca gattgatccc
     2401 agctccatag aaagagccta tgcagctctt ggactaccct atcaagtaaa tcagatgccg
     2461 acacaacccc aggtgcaagc aaagaaccag cagaatcagc agcctgggca gtctccccaa
     2521 ggcatgcggc ccatgagcaa catgagtgct agtcctatgg gagtaaatgg aggtgtagga
     2581 gttcaaacgc cgagtcttct ttctgactca atgttgcatt cagccataaa ttctcaaaac
     2641 ccaatgatga gtgaaaatgc cagtgtgccc tccctgggtc ctatgccaac agcagctcaa
     2701 ccatccacta ctggaattcg gaaacagtgg cacgaagata ttactcagga tcttcgaaat
     2761 catcttgttc acaaactcgt ccaagccata tttcctacgc cggatcctgc tgctttaaaa
     2821 gacagacgga tggaaaacct agttgcatat gctcggaaag ttgaagggga catgtatgaa
     2881 tctgcaaaca atcgagcgga atactaccac cttctagctg agaaaatcta taagatccag
     2941 aaagaactag aagaaaaacg aaggaccaga ctacagaagc agaacatgct accaaatgct
     3001 gcaggcatgg ttccagtttc catgaatcca gggcctaaca tgggacagcc gcaaccagga
     3061 atgacttcta atggccctct acctgaccca agtatgatcc gtggcagtgt gccaaaccag
     3121 atgatgcctc gaataactcc acaatctggt ttgaatcaat ttggccagat gagcatggcc
     3181 cagcccccta ttgtaccccg gcaaacccct cctcttcagc accatggaca gttggctcaa
     3241 cctggagctc tcaacccgcc tatgggctat gggcctcgta tgcaacagcc ttccaaccag
     3301 ggccagttcc ttcctcagac tcagttccca tcacagggaa tgaatgtaac aaatatccct
     3361 ttggctccgt ccagcggtca agctccagtg tctcaagcac aaatgtctag ttcttcctgc
     3421 ccggtgaact ctcctataat gcctccaggg tctcagggga gccacattca ctgtccccag
     3481 cttcctcaac cagctcttca tcagaattca ccctcgcctg tacctagtcg tacccccacc
     3541 cctcaccata ctcccccaag cataggggct cagcagccac cagcaacaac aattccagcc
     3601 cctgttccta cacctcctgc catgccacct gggccacagt cccaggctct acatccccct
     3661 ccaaggcaga cacctacacc accaacaaca caacttcccc aacaagtgca gccttcactt
     3721 cctgctgcac cttctgctga ccagccccag cagcagcctc gctcacagca gagcacagca
     3781 gcgtctgttc ctaccccaac agcaccgctg cttcctccgc agcctgcaac tccactttcc
     3841 cagccagctg taagcattga aggacaggta tcaaatcctc catctactag tagcacagaa
     3901 gtgaattctc aggccattgc tgagaagcag ccttcccagg aagtgaagat ggaggccaaa
     3961 atggaagtgg atcaaccaga accagcagat actcagccgg aggatatttc agagtctaaa
     4021 gtggaagact gtaaaatgga atctaccgaa acagaagaga gaagcactga gttaaaaact
     4081 gaaataaaag aggaggaaga ccagccaagt acttcagcta cccagtcatc tccggctcca
     4141 ggacagtcaa agaaaaagat tttcaaacca gaagaactac gacaggcact gatgccaact
     4201 ttggaggcac tttaccgtca ggatccagaa tcccttccct ttcgtcaacc tgtggaccct
     4261 cagcttttag gaatccctga ttactttgat attgtgaaga gccccatgga tctttctacc
     4321 attaagagga agttagacac tggacagtat caggagccct ggcagtatgt cgatgatatt
     4381 tggcttatgt tcaataatgc ctggttatat aaccggaaaa catcacgggt atacaaatac
     4441 tgctccaagc tctctgaggt ctttgaacaa gaaattgacc cagtgatgca aagccttgga
     4501 tactgttgtg gcagaaagtt ggagttctct ccacagacac tgtgttgcta cggcaaacag
     4561 ttgtgcacaa tacctcgtga tgccacttat tacagttacc agaacaggta tcatttctgt
     4621 gagaagtgtt tcaatgagat ccaaggggag agcgtttctt tgggggatga cccttcccag
     4681 cctcaaacta caataaataa agaacaattt tccaagagaa aaaatgacac actggatcct
     4741 gaactgtttg ttgaatgtac agagtgcgga agaaagatgc atcagatctg tgtccttcac
     4801 catgagatca tctggcctgc tggattcgtc tgtgatggct gtttaaagaa aagtgcacga
     4861 actaggaaag aaaataagtt ttctgctaaa aggttgccat ctaccagact tggcaccttt
     4921 ctagagaatc gtgtgaatga ctttctgagg cgacagaatc accctgagtc aggagaggtc
     4981 actgttagag tagttcatgc ttctgacaaa accgtggaag taaaaccagg catgaaagca
     5041 aggtttgtgg acagtggaga gatggcagaa tcctttccat accgaaccaa agccctcttt
     5101 gcctttgaag aaattgatgg tgttgacctg tgcttctttg gcatgcatgt tcaagagtat
     5161 ggctctgact gccctccacc caaccagagg agagtataca tatcttacct cgatagtgtt
     5221 catttcttcc gtcctaaatg cttgaggact gcagtctatg ccgaaatcct aattggatat
     5281 ttagcggccg tcaagaaatc cggagccaca acaggggcca tttgggcatg tccaccaagt
     5341 gagggagatg attatatctt ccattgccat cctcctgacc agaagatacc caagcccaag
     5401 cgactgcagg aatggtacaa aaaaatgctt gacaaggctg tatcagagcg tattgtccat
     5461 gactacaagg atatttttaa acaagctact gaagatagat taacaagtgc aaaggaattg
     5521 ccttatttcg agggtgattt ctggcccaat gttctggaag aaagcattaa ggaactggaa
     5581 caggaggaag aagagagaaa acgagaggaa aacaccagca atgaaagcac agatgtgacc
     5641 aagggagaca gcaaaaatgc taaaaagaag aataataaga aaaccagcaa aaataagagc
     5701 agcctgagta ggggcaacaa gaagaaaccc gggatgccca atgtatctaa cgacctctca
     5761 cagaaactat atgccaccat ggagaagcat aaagaggtct tctttgtgat ccgcctcatt
     5821 gctggccctg ctgccaactc cctgcctccc attgttgatc ctgatcctct catcccctgc
     5881 gatctgatgg atggtcggga tgcgtttctc acgctggcaa gggacaagca cctggagttc
     5941 tcttcactcc gaagagccca gtggtccacc atgtgcatgc tggtggagct gcacacgcag
     6001 agccaggacc gctttgtcta cacctgcaat gaatgcaagc accatgtgga gacacgctgg
     6061 cactgtactg tctgtgagga ttatgacttg tgtatcacct gctataacac taaaaaccat
     6121 gaccacaaaa tggagaaact aggccttggc ttagatgatg agagcaacaa ccagcaggct
     6181 gcagccaccc agagcccagg cgattctcgc cgcctgagta tccagcgctg catccagtct
     6241 ctggtccatg cttgccagtg tcggaatgcc aattgctcac tgccatcctg ccagaagatg
     6301 aagcgggttg tgcagcatac caagggttgc aaacggaaaa ccaatggcgg gtgccccatc
     6361 tgcaagcagc tcattgccct ctgctgctac catgccaagc actgccagga gaacaaatgc
     6421 ccggtgccgt tctgcctaaa catcaagcag aagctccggc agcaacagct gcagcaccga
     6481 ctacagcagg cccaaatgct tcgcaggagg atggccagca tgcagcggac tggtgtggtt
     6541 gggcagcaac agggcctccc ttcccccact cctgccactc caacgacacc aactggccaa
     6601 cagccaacca ccccgcagac gccccagccc acttctcagc ctcagcctac ccctcccaat
     6661 agcatgccac cctacttgcc caggactcaa gctgctggcc ctgtgtccca gggtaaggca
     6721 gcaggccagg tgacccctcc aacccctcct cagactgctc agccacccct tccagggccc
     6781 ccacctgcag cagtggaaat ggcaatgcag attcagagag cagcggagac gcagcgccag
     6841 atggcccacg tgcaaatttt tcaaaggcca atccaacacc agatgccccc gatgactccc
     6901 atggccccca tgggtatgaa cccacctccc atgaccagag gtcccagtgg gcatttggag
     6961 ccagggatgg gaccgacagg gatgcagcaa cagccaccct ggagccaagg aggattgcct
     7021 cagccccagc aactacagtc tgggatgcca aggccagcca tgatgtcagt ggcccagcat
     7081 ggtcaacctt tgaacatggc tccacaacca ggattgggcc aggtaggtat cagcccactc
     7141 aaaccaggca ctgtgtctca acaagcctta caaaaccttt tgcggactct caggtctccc
     7201 agctctcccc tgcagcagca acaggtgctt agtatccttc acgccaaccc ccagctgttg
     7261 gctgcattca tcaagcagcg ggctgccaag tatgccaact ctaatccaca acccatccct
     7321 gggcagcctg gcatgcccca ggggcagcca gggctacagc cacctaccat gccaggtcag
     7381 cagggggtcc actccaatcc agccatgcag aacatgaatc caatgcaggc gggcgttcag
     7441 agggctggcc tgccccagca gcaaccacag cagcaactcc agccacccat gggagggatg
     7501 agcccccagg ctcagcagat gaacatgaac cacaacacca tgccttcaca attccgagac
     7561 atcttgagac gacagcaaat gatgcaacag cagcagcaac agggagcagg gccaggaata
     7621 ggccctggaa tggccaacca taaccagttc cagcaacccc aaggagttgg ctacccacca
     7681 cagcagcagc agcggatgca gcatcacatg caacagatgc aacaaggaaa tatgggacag
     7741 ataggccagc ttccccaggc cttgggagca gaggcaggtg ccagtctaca ggcctatcag
     7801 cagcgactcc ttcagcaaca gatggggtcc cctgttcagc ccaaccccat gagcccccag
     7861 cagcatatgc tcccaaatca ggcccagtcc ccacacctac aaggccagca gatccctaat
     7921 tctctctcca atcaagtgcg ctctccccag cctgtccctt ctccacggcc acagtcccag
     7981 cccccccact ccagtccttc cccaaggatg cagcctcagc cttctccaca ccacgtttcc
     8041 ccacagacaa gttccccaca tcctggactg gtagctgccc aggccaaccc catggaacaa
     8101 gggcattttg ccagcccgga ccagaattca atgctttctc agcttgctag caatccaggc
     8161 atggcaaacc tccatggtgc aagcgccacg gacctgggac tcagcaccga taactcagac
     8221 ttgaattcaa acctctcaca gagtacacta gacatacacc accatcacca ccattagtga
     8281 tactaagctt aagtttaaac cgctgatcag cctcgactgt gccttctagt tgccagccat
     8341 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8401 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8461 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     8521 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     8581 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8641 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8701 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8761 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8821 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8881 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8941 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     9001 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     9061 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     9121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     9301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9361 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     9421 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     9481 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     9541 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     9601 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     9661 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     9721 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     9781 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     9841 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     9901 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     9961 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
    10021 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
    10081 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
    10141 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
    10201 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
    10261 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    10321 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    10381 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10441 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10501 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
    10561 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    10621 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    10681 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10741 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    10801 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10861 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10921 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10981 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11041 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11101 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    11161 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    11221 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11281 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    11341 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11401 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11461 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    11521 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    11581 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    11641 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    11701 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11761 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11821 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11881 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11941 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12001 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    12061 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    12121 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    12181 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    12241 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    12301 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    12361 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12421 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12481 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    12541 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    12601 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12661 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    12721 ga
//
