LOCUS       sequence-177335-        6365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Flag-MCPIP1(1-555).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Liang J, Saad Y, Lei T, Wang J, Qi D, Yang Q, Kolattukudy PE, Fu M
  TITLE     MCP-induced protein 1 deubiquitinates TRAF proteins and negatively 
            regulates JNK and NF-kappaB signaling.
  JOURNAL   J Exp Med. 2010 Dec 20;207(13):2959-73. doi: 10.1084/jem.20092641. 
            Epub 2010 Nov 29.
  PUBMED    21115689
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20092641"; journalName: "J Exp Med"; date: "2010-12-20-
            20"; volume: "207"; issue: "13"; pages: "2959-73"
COMMENT     Sequence Label: Flag-MCPIP1(1-555)
FEATURES             Location/Qualifiers
     source          1..6365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(147..167)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        152..481
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      332..467
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(519..538)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(520..538)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(552..568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(552..568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(565..587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    576..592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(600..606,607..624,625..630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    645..666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(783..800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(954..1542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1034..1053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1713..2504,2505..2573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2336..2355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2574..2678)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2705..3160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2792..2811)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3002..3023
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3286..3308
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3300..3317
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3301..3317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        3478..3857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3858..4061
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4012..4032
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4122..4145
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    join(5866..6365,1..123)
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6065..6084)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gcaggaggac gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt
       61 gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga
      121 attgggtacg cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
      181 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
      241 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      301 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
      361 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
      421 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
      481 aaagctcctc gaggaactga aaaaccagaa agttaattcc ctatagtgag tcgtattaaa
      541 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      601 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      661 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      721 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      781 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      841 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      901 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      961 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1021 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1081 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1141 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1201 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1261 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1321 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1381 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1441 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1501 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1561 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1621 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1681 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1741 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1801 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1861 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1921 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1981 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2041 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2101 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2161 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2221 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2281 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2341 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2401 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2461 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2521 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2581 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2641 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2701 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2761 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2821 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2881 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2941 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3001 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3061 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3121 tttaacgcga attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc
     3181 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     3241 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     3301 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
     3361 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     3421 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
     3481 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     3541 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3601 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3661 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3721 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3781 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
     3841 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
     3901 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3961 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
     4021 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     4081 attgatctac catggcccac aaccaccgcc acaagcacgc cgactacaaa gacgatgacg
     4141 acaagcttgc ggccgcgaat tcaatgagtg gcccctgtgg agagaagcct gtcctggaag
     4201 ccagccccac catgagtctg tgggaatttg aggacagcca cagccgtcag ggcaccccaa
     4261 ggccgggtca agagctggcc gctgaggagg cctcggccct ggaactgcag atgaaggtgg
     4321 acttcttccg gaagctgggc tattcatcca cggagatcca cagcgtcctg cagaagctgg
     4381 gcgtccaggc agacaccaac acggtgctgg gtgagctggt gaaacacggg acagccaccg
     4441 agcgggagcg ccagacctca ccggacccct gccctcagct ccctctagtc ccgcggggtg
     4501 gtggcacccc taaggctccc aacctggagc ctccactccc agaagaggaa aaggagggca
     4561 gcgacctgag accagtggtc atcgatggga gcaacgtggc catgagccat gggaacaagg
     4621 aggtcttctc ctgccggggc atcctgctgg cagtgaactg gtttctggag cggggccaca
     4681 cagacatcac agtgtttgtg ccatcctgga ggaaggagca gcctcggccc gacgtgccca
     4741 tcacagacca gcacatcctg cgggaactgg agaagaagaa gatcctggtg ttcacaccat
     4801 cacgacgcgt gggtggcaag cgggtggtgt gctatgacga cagattcatt gtgaagctgg
     4861 cctacgagtc tgacgggatc gtggtttcca acgacacata ccgtgacctc caaggcgagc
     4921 ggcaggagtg gaagcgcttc atcgaggagc ggctgctcat gtactccttc gtcaatgaca
     4981 agtttatgcc ccctgatgac ccactgggcc ggcacgggcc cagcctggac aacttcctgc
     5041 gtaagaagcc actcactttg gagcacagga agcagccgtg tccctatgga aggaaatgca
     5101 cctatgggat caagtgccga ttcttccacc cagagcggcc aagctgcccc cagcgctctg
     5161 tggcagatga gctccgtgcc aatgctctcc tctcaccccc cagagcccca agcaaggaca
     5221 aaaatggccg gcggccttca ccttcatccc agtccagctc tctgctaaca gagagtgagc
     5281 agtgcagcct ggatgggaag aagctggggg cccaggcatc cccagggtcc cgccaagagg
     5341 gtctaacaca gacctatgcc ccatcaggca ggagcctcgc acctagcggg ggcagtggca
     5401 gcagctttgg gcccacagac tggctcccac agacgctgga ctcactcccg tacgtctccc
     5461 aggattgcct ggactcgggc attggctccc tggagagcca gatgtcggaa ctttgggggg
     5521 ttcgaggagg aggccctggt gagccgggcc caccccgagc cccttacacg ggctacagtc
     5581 cctatggatc tgagctccca gccaccgcag ccttctctgc ctttggccgg gccatgggtg
     5641 ctggccactt cagtgtccct gccgactacc cacccgcgcc ccctgccttt ccacctcgag
     5701 agtactggtc tgaaccatac ccactgcccc cacccacatc agtccttcag gagcccccag
     5761 tgcagagccc aggggctggc aggagcccgt ggggcagggc aggcagcctg gccaaggagc
     5821 aggccagcta atctagatat cggtaccgga tccagatctg atcccgggtg gcatccctgt
     5881 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     5941 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     6001 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     6061 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     6121 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     6181 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     6241 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     6301 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     6361 tacca
//
