LOCUS       sequence-177340-        6460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Flag-MCPIP1(delta ZF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Liang J, Saad Y, Lei T, Wang J, Qi D, Yang Q, Kolattukudy PE, Fu M
  TITLE     MCP-induced protein 1 deubiquitinates TRAF proteins and negatively 
            regulates JNK and NF-kappaB signaling.
  JOURNAL   J Exp Med. 2010 Dec 20;207(13):2959-73. doi: 10.1084/jem.20092641. 
            Epub 2010 Nov 29.
  PUBMED    21115689
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20092641"; journalName: "J Exp Med"; date: "2010-12-20-
            20"; volume: "207"; issue: "13"; pages: "2959-73"
COMMENT     Sequence Label: Flag-MCPIP1(delta ZF)
FEATURES             Location/Qualifiers
     source          1..6460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             645..668
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2484..3106
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2683..2702)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3130..3150)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3135..3464
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3315..3450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3502..3521)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3503..3521)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3535..3551)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3535..3551)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3548..3570)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3559..3575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3583..3589,3590..3607,3608..3613))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3628..3649
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3766..3783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3937..4525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4017..4036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4696..5487,5488..5556))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5319..5338
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5557..5661)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5688..6143
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5775..5794)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5985..6006
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6269..6291
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6283..6300
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6284..6300
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattgatc taccatggcc cacaaccacc gccacaagca cgccgactac aaagacgatg
      661 acgacaagct tatgagtggc ccctgtggag agaagcctgt cctggaagcc agccccacca
      721 tgagtctgtg ggaatttgag gacagccaca gccgtcaggg caccccaagg ccgggtcaag
      781 agctggccgc tgaggaggcc tcggccctgg aactgcagat gaaggtggac ttcttccgga
      841 agctgggcta ttcatccacg gagatccaca gcgtcctgca gaagctgggc gtccaggcag
      901 acaccaacac ggtgctgggt gagctggtga aacacgggac agccaccgag cgggagcgcc
      961 agacctcacc ggacccctgc cctcagctcc ctctagtccc gcggggtggt ggcaccccta
     1021 aggctcccaa cctggagcct ccactcccag aagaggaaaa ggagggcagc gacctgagac
     1081 cagtggtcat cgatgggagc aacgtggcca tgagccatgg gaacaaggag gtcttctcct
     1141 gccggggcat cctgctggca gtgaactggt ttctggagcg gggccacaca gacatcacag
     1201 tgtttgtgcc atcctggagg aaggagcagc ctcggcccga cgtgcccatc acagaccagc
     1261 acatcctgcg ggaactggag aagaagaaga tcctggtgtt cacaccatca cgacgcgtgg
     1321 gtggcaagcg ggtggtgtgc tatgacgaca gattcattgt gaagctggcc tacgagtctg
     1381 acgggatcgt ggtttccaac gacacatacc gtgacctcca aggcgagcgg caggagtgga
     1441 agcgcttcat cgaggagcgg ctgctcatgt actccttcgt caatgacaag tttatgcccc
     1501 ctgatgaccc actgggccgg cacgggccca gcctggacaa cttcctgcgt aagaagccac
     1561 tcactttgga gcacaggaag cagccgtgtc cctatggaag gaaatgcacc tatgggatcg
     1621 ggggccgatt cttccaccca gagcggccaa gctgccccca gcgctctgtg gcagatgagc
     1681 tccgtgccaa tgctctcctc tcacccccca gagccccaag caaggacaaa aatggccggc
     1741 ggccttcacc ttcatcccag tccagctctc tgctaacaga gagtgagcag tgcagcctgg
     1801 atgggaagaa gctgggggcc caggcatccc cagggtcccg ccaagagggt ctaacacaga
     1861 cctatgcccc atcaggcagg agcctcgcac ctagcggggg cagtggcagc agctttgggc
     1921 ccacagactg gctcccacag acgctggact cactcccgta cgtctcccag gattgcctgg
     1981 actcgggcat tggctccctg gagagccaga tgtcggaact ttggggggtt cgaggaggag
     2041 gccctggtga gccgggccca ccccgagccc cttacacggg ctacagtccc tatggatctg
     2101 agctcccagc caccgcagcc ttctctgcct ttggccgggc catgggtgct ggccacttca
     2161 gtgtccctgc cgactaccca cccgcgcccc ctgcctttcc acctcgagag tactggtctg
     2221 aaccataccc actgccccca cccacatcag tccttcagga gcccccagtg cagagcccag
     2281 gggctggcag gagcccgtgg ggcagggcag gcagcctggc caaggagcag gccagcgtgt
     2341 atactaagct gtgtggtgtg tttcccccgc acctggtgga ggctgtgatg gggcgcttcc
     2401 cacagctcct ggacccccag cagctggctg ccgagatcct ctcctacaag tcccagcacc
     2461 ccagtgagta aagatctgat cccgggtggc atccctgtga cccctcccca gtgcctctcc
     2521 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca
     2581 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga
     2641 gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct
     2701 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
     2761 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat
     2821 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct
     2881 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca
     2941 ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag gacgtccaga
     3001 cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc ttgcttggca
     3061 ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt acgcggccag
     3121 cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     3181 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     3241 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     3301 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3361 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3421 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ctcgaggaac
     3481 tgaaaaacca gaaagttaat tccctatagt gagtcgtatt aaattcgtaa tcatgtcata
     3541 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3601 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3661 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3721 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3781 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4501 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4561 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4621 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4681 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4741 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4801 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4861 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4921 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4981 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5041 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5101 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5161 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5221 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5281 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5341 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5401 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5461 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5521 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5581 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5641 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
     5701 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5761 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5821 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5881 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5941 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6001 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6061 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6121 caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg
     6181 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     6241 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     6301 gccaagctga tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat
     6361 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
     6421 tacatttata ttggctcatg tccaatatga ccgccatgtt
//
